Effect of Xifeng Zhidong Decoction on inflammatory reaction of microglia induced by lipopolysaccharide in mice

Shasha LIU; Jing CHEN; Sina LIU; Tingwei LONG; Xinqiang NI; Rong MA

doi:10.16305/j.1007-1334.2022.2010113

您当前的位置：

首页 >

文章列表页 >

Effect of Xifeng Zhidong Decoction on inflammatory reaction of microglia induced by lipopolysaccharide in mice

更新时间：2022-10-09

- Effect of Xifeng Zhidong Decoction on inflammatory reaction of microglia induced by lipopolysaccharide in mice
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 7, Pages: 89-96(2022)
- 作者机构：
  
  1.深圳市中医院儿科（广东深圳 518000）
  2.杭州市儿童医院中医科（浙江杭州 310000）
  3.天津中医药大学第一附属医院儿科（天津 300192）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2010113
  CLC：
扫描看全文
刘莎莎,陈景,刘斯娜,等.熄风制动汤对脂多糖诱导小鼠小胶质细胞炎症反应的作用研究[J].上海中医药杂志,2022,56(7):89-96.

LIU Shasha,CHEN Jing,LIU Sina,et al.Effect of Xifeng Zhidong Decoction on inflammatory reaction of microglia induced by lipopolysaccharide in mice[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(7):89-96.
刘莎莎,陈景,刘斯娜,等.熄风制动汤对脂多糖诱导小鼠小胶质细胞炎症反应的作用研究[J].上海中医药杂志,2022,56(7):89-96. DOI： 10.16305/j.1007-1334.2022.2010113.

LIU Shasha,CHEN Jing,LIU Sina,et al.Effect of Xifeng Zhidong Decoction on inflammatory reaction of microglia induced by lipopolysaccharide in mice[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(7):89-96. DOI： 10.16305/j.1007-1334.2022.2010113.

摘要

目的,2,探究熄风制动汤对脂多糖（LPS）诱导的小鼠小胶质细胞（BV-2细胞）炎症反应的作用。,方法,2,将BV-2细胞设置为Control组、LPS组及熄风制动汤组。Control组加入达尔伯克改良伊格尔（DMEM）培养基，LPS组以0.1 mg/L浓度对BV-2细胞进行诱导炎症反应，熄风制动汤组分别以浓度为12.5、25、50、100、200、400 mg·L,-1,的熄风制动汤进行相应干预。采用CCK-8法观察熄风制动汤对BV-2细胞活力的影响；ELISA法检测细胞上清中一氧化氮（NO）水平；real-time PCR检测BV-2细胞炎症因子水平的表达；Western blot法及免疫荧光染色法检测一氧化氮诱导合酶（iNOS）、精氨酸-1（Arg-1）蛋白的表达。,结果,2,熄风制动汤不同剂量组或LPS组培养BV-2细胞后，对其活力无明显影响（,P>,0.05）。与Control组比较，LPS组NO的释放显著增加（,P,<,0.001）； M1型小胶质细胞产物肿瘤坏死因子-α（,TNF,-,α,）、白介素-6（,IL,-,6,）、白介素-1β（,IL,-,1β,）mRNA表达增加（,P,<,0.001），M2型小胶质细胞产物转化生长因子-β（,TGF,-,β,）mRNA表达降低（,P<,0.05）， M1型标志物iNOS的蛋白表达增加（,P,<,0.01），M2型标志物Arg-1蛋白的表达降低（,P<,0.05）。与LPS组比较，熄风制动汤组（100、200、400 mg·L,-1,）NO的释放（,P,<,0.001）抑制，M1型小胶质细胞产物,TNF,-,α,（,P,<,0.001）、,IL,-,6, mRNA（,P<,0.05）表达降低；熄风制动汤组（400 mg·L,-1,）M2型小胶质细胞产物,IL,-,10,、,TGF,-,β, mRNA表达增加（,P,<,0.05）；熄风制动汤组（200 mg·L,-1,）M1型标志物iNOS蛋白表达降低（,P,<,0.01），熄风制动汤组（100 mg·L,-1,）M2型标志物Arg-1蛋白表达增加（,P,<,0.05）。,结论,2,熄风制动汤可以抑制LPS诱导的BV-2细胞向M1损伤型转化，促进向M2修复型转化。

Abstract

Objective,2,To explore the effect of Xifeng Zhidong Decoction on inflammatory reaction of mouse microglia （BV-2 cells） induced by lipopolysaccharide （LPS）.,Methods,2,The microglia of mice were divided into three groups： control group， LPS group and Xifeng Zhidong Decoction group. The DMEM medium was added to the control group. LPS was used to induce inflammation on microglia at a concentration of 0.1 mg·L,-1, in the LPS group， and intervention was performed by Xifeng Zhidong Decoction with a concentration of 12.5， 25， 50， 100， 200 and 400 mg·L,-1 ,respectively in the Xifeng Zhidong Decoction group. The effect of Xifeng Zhidong Decoction on the viability of microglia was observed by cell proliferative toxicity test （CCK-8）. The level of nitric oxide （NO） in the supernatant was detected by enzyme linked immunosorbent assay （ELISA）， and the expression of inflammatory factors in microglia was detected by fluorescence quantitative polymerase chain reaction （real-time PCR）. Western blot and immunofluorescence staining were used to detect the expression of nitric oxide-induced synthase （iNOS） and arginine-1 （Arg-1） proteins.,Results,2,There was no significant effect on the activity of microglia in different doses of Xifeng Zhidong group or LPS group （,P,>,0.05）. Compared with the control group， the NO content in microglia supernatant of the LPS group increased significantly（,P,<,0.001）； the mRNA expression of ,TNF,-,α,， ,IL,-,6,， ,IL,-,1β, of M1 type microglia increased significantly（,P,<,0.001）， but the mRNA expression of ,TGF,-,β, of M2 type microglia decreased （,P,<,0.05）； the protein expression of M1 marker iNOS increased （,P,<,0.01）， but the expression of M2 marker Arg-1 protein decreased （,P,<,0.05）. Compared with the LPS group， Xifeng Zhidong Decoction at 100， 200， and 400 mg·L,-1, could significantly inhibit the release of NO（,P,<,0.001）， decrease the expression of ,TNF,-,α， IL,-,6, mRNA of M1 type microglia（,P,<,0.001， ,P,<,0.05）. Xifeng Zhidong Decoction increased the expression of ,IL,-,10, and ,TGF,-,β ,mRNA of M2 type microglia at 400 mg·L,-1,（,P,<,0.05）， reduced the expression of M1 type marker iNOS protein at 200 mg·L,-1, （,P,<,0.01）， and increased the expression of M2 type marker Arg-1 protein at 100 mg·L,-1, （,P,<,0.05）.,Conclusion,2,Xifeng Zhidong Decoction can inhibit the transformation of microglia to M1 damaged type induced by LPS， and promote the transformation to M2 repair type.

关键词

抽动障碍熄风制动汤小胶质细胞脂多糖炎症反应中药研究

Keywords

tic disorderXifeng Zhidong Decoctonmicrogliallipopolysaccharideinflammatory reactiontraditional Chinese herbal medicine research

references

BACHILLER S， JIMÉNEZ-FERRER I， PAULUS A， et al. Microglia in neurological diseases： A road map to brain-disease dependent-inflammatory response［J］. Front Cell Neurosci， 2018， 12： 488.

MARTINO D， ZIS P， BUTTIGLIONE M. The role of immune mechanisms in Tourette syndrome［J］. Brain Res， 2015， 1617（18）： 126-143.

LENNINGTON J B， COPPOLA G， KATAO-SASAKI Y， et al. Transcriptome analysis of the human striatum in tourette syndrome［J］. Biol Psychiatry， 2016， 79（5）： 372-382.

KUMAR A， WILLIAMS M T， CHUGANI H T. Evaluation of basal ganglia and thalam inflammation in children with pediatric autoimmune neuropsychiatric disorders associated with streptococcal infection and tourette syndromes：a positron emission tomographic （PET） study using 11C-［R］-PK11195［J］. J Child Neurol， 2015， 30（6）： 749-756.

马碧涛. 基于Micro-PET/CT平台的抽动障碍发病机制及祛风止动方干预的机理研究［D］.上海：上海交通大学，2011.

张梦娇，王春丽，黄亚若，等. 玉屏风散对抽动障碍模型大鼠的影响［J］. 药学与临床研究，2019， 27（3）： 166-170.

刘莎莎，马融，张喜莲，等. 佐金平木法在儿童肝系疾病治疗中的应用［J］. 中医杂志，2015， 56（7）： 618-619.

刘莎莎，陈汉江，马融. 运用熄风定摇汤加味治疗颈性抽动症体会［J］. 中医杂志，2013， 54（23）： 2054-2055.

曹静，王萌芽. 栀子苷对睡眠剥夺大鼠学习记忆的影响［J］. 安徽医学，2020， 5（5）： 497-500.

任嘉彦. 栀子苷对实验性自身免疫性脑脊髓炎小鼠星型胶质细胞的影响［D］. 长春：长春中医药大学，2019.

赵铭，李冬，朱迅，等. 小胶质细胞在中枢神经系统疾病中作用的研究进展［J］. 现代免疫学，2018， 38（5）： 425-428.

YANG Q Q， ZHOU J W. Neuroinflammation in the central nervous system： Symphony of glial cells［J］. Glia， 2019， 67（6）： 1017-1035.

范菲，韩斐，汪琼. 抽动障碍儿童血清TNF-α和IL-6的表达［J］. 江苏医药，2017， 43（14）： 1005-1007.

张紫萱，田绍文，游咏. 神经炎症在神经退行性疾病和精神疾病中的病理生理作用［J］.中南医学科学杂志，2017， 45（3）： 312-314.

吴杰. 黄芩苷、栀子苷及其配伍调控缺血性脑卒中后小胶质细胞M1/M2分化发挥神经元保护作用［D］. 咸阳：陕西中医药大学，2020.

张桂莲，吴海琴，张茹，等. 肿瘤坏死因子、内皮素在脑卒中血及脑脊液中的表达及关系探讨［J］. 陕西医学杂志，2001， 30（3）： 151-153．

狄政莉，万琪，王洪典，等．IL-1β和IL-6在脑缺血再灌注微血管内皮细胞炎症反应中的作用［J］. 西安交通大学学报（医学版），2001， 22（5）： 432-434．

王珺. 加味泻黄散对多发性抽动症模型小鼠行为学及TNF-α、IL-6的影响［D］. 成都：成都中医药大学，2016.

康东坤，高外毛，张红珍，等. 黄芪总皂苷对脂多糖诱导的BV2小胶质细胞的抗炎机制［J］. 中国病理生理杂志，2022， 38（2）： 276-283.

张伟，孙伟明，徐家淳，等. 中药调控小胶质细胞功能治疗阿尔茨海默病的机制研究进展［J］. 环球中医药，2021， 14（11）： 2091-2095.

贺春香，于文静，杨苗，等. 黄芩苷通过TREM2/TLR4/NF-κB信号通路抑制脂多糖/干扰素γ诱导的BV2细胞炎症反应［J］.中国中药杂志，2022， 47（6）： 1603-1610.

陈璐，朱丽蓉，蒋珊，等. 葛根素通过抑制小胶质细胞TLR4/NF-κB介导的炎症反应治疗神经根性疼痛［J］. 中国病理生理杂志，2021， 37（4）： 667-672.

Views

479

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Xinqian Ganjie Decoction on lncRNA SNHG16， EGFR and MUC5AC mRNA expression in chronic rhinosinusitis mice

Mechanism of Yucan Granules improving podocyte damage and treating diabetic nephropathy

Mechanism of Huangqi Decoction against liver fibrosis by network pharmacological analysis combined with related cell experiment

Baicalin modulates gut microbiota and inhibits liver metastasis of colorectal cancer induced by high⁃fat diet

Quantitative analysis of notoginsenoside R1， ginsenoside Rg1 and ginsenoside Rb1 by HPLC and their intestinal mucosal permeability

Related Author

No data

Related Institution

Department of Otolaryngology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Vascular Surgery， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Nephrology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Institute of Interdisciplinary Integrative Medicine Research， Shanghai University of Traditional Chinese Medicine

School of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine

⁰