FANG Hong,CHEN Ximei,GU Lihua,et al.Study on improvement of TLC identification of Zhuling Jianpi Capsule[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(7):102-106.
FANG Hong,CHEN Ximei,GU Lihua,et al.Study on improvement of TLC identification of Zhuling Jianpi Capsule[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(7):102-106. DOI: 10.16305/j.1007-1334.2022.2008052.
Study on improvement of TLC identification of Zhuling Jianpi Capsule
Objective,2,To improve the TLC identification of Zhuling Jianpi Capsule.,Methods,2,The supernatants of the inner content of Zhuling Jianpi Capsule via successive ultrasonication with ,n,-hexane following with mehanol were separately used for the identification of Atractylodis Macrocephalae Rhizoma and for the identifications of Paeoniae Radix Alba, Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium in Zhuling Jianpi Capsule. The residual methanol filtrate was evaporated to dryness and dissolved with water, applied to D101 macroporous resin column (inner diameter 2 cm, column length 15 cm), eluted successively with 10% methanol, 50% methanol, 80% methanol and 90% methanol. The 50% methanol eluate was used for identification of Linderae Radix and Saposhnikoviae Radix, 80% methanol eluate was used for identification of Glycyrrhizae Radix et Rhizoma, and 90% methanol eluate for identification of Magnoliae Officinalis Cortex.,Results,2,Eight TLC identification methods were accomplished, and three TLC identification methods for Saposhnikoviae Radix, Linderae Radix and Glycyrrhizae Radix et Rhizomawere were newly established and three TLC identification methods for Magnoliae Officinalis Cortex, Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium were improved. Paeoniflorin was used as the marker of Paeoniae Radix Alba, norisoboldine as the marker of Linderae Radix, prim-O-glucosylcimifugin and 5-O-methylvisammioside as the markers of Saposhnikoviae Radix, and the mixture of chloroform and methanol (4∶1) was used as the same mobile phase for three of them; berberine hydrochloride was used as the marker of Phellodendri Chinensis Cortex and hesperidin as the marker of Citri Reticulatae Pericarpium, and the low-layer solution of the mixture of chloroform, methanol and water (37∶17∶5) was used as the same mobile phase for both of them; Magnolol and honokiol were used as the markers of Magnoliae Officinalis Cortex and the mixture of toluene and methanol (10∶1) as the mobile phase; liquiritin and monoammonium glycyrrhizinate were used as the markers of Glycyrrhizae Radix et Rhizoma, and the mixture of ethyl acetate, formic acid, glacial acetic acid, and water (15∶1∶1∶2) as the mobile phase.,Conclusion,2,A systematic sample test solution preparation strategy is implemented for concurrently preparing 4 test solutions for eight TLC identifications, the modified TLC identification methods are more suitable to control the quality of Zhuling Jianpi Capsule.
关键词
肠易激综合征术苓健脾胶囊薄层色谱质量标准中药研究
Keywords
irritable bowel syndromeZhuling Jianpi CapsuleTLC identificationquality standardtraditional Chinese herbal medicine research
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Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines, The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai R&D Center for Standardization of Chinese Medicines
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