Objective,2,To establish a method for qualitative identification and quality control of Alismatis Rhizoma, Salviae miltiorrhizae Radix et Rhizoma, Poria and Cynomorii Herba in Shenkangning Capsule by thin layer chromatography (TLC) .,Methods,2,The single-factor test method was used to systematically investigate the factors of TLC conditions, including different developing solvents, chromogenic reagents, spot volume, temperature, humidity, and to determine the optimal TLC conditions for the identification of Alismatis Rhizoma, Salviae miltiorrhizae Radix et Rhizoma, Poria and Cynomorii Herba.,Results,2,The TLC conditions for Alismatis Rhizoma and Salviae miltiorrhizae Radix et Rhizoma were to develop on silica gel G thin-layer plate with n-hexane-ethyl acetate-formic acid (7∶4∶0.6) as the developing solvent. After the plate was taken out and dried, the fluorescent spots were observed under ultraviolet lamp (365 nm) , then sprayed with 2% phosphomolybdate ethanol solution, heated at 105℃ until the spots became clear. The TLC conditions for Poria were to develop on silica gel G thin-layer plate with n-hexane-ethyl acetate-formic acid (7∶4∶0.4) as the developing solvent. After the plate was taken out and dried, the fluorescent spots were observed under ultraviolet lamp (365 nm) . The TLC conditions for Cynomorii Herba were to develop on silica gel G thin-layer plate with cyclohexane-ethyl acetate-methanol-formic acid (8∶6∶1∶1) as the developing solvent After the plate was taken out and dried, it was soaked in 0.04% DPPH absolute ethanol solution. The spots were clear under daylight after the plate was dried again.,Conclusions,2,Thin layer chromatography features clear identification spots, strong specificity, negative control without interference. The method is also simple, convenient and has good repeatability, which can be adopted for the quality control of Shenkangning capsule.