Study on plasma protein binding rates of bufalin,cinobufagin and resibufogenin in different kinds of toad skin extracts

Jingjing SUN; Shan LING; Zhongfang HU; Wei YIN; Jiaxin WU; Qingchun NI

doi:10.16305/j.1007-1334.2021.2103056

您当前的位置：

首页 >

文章列表页 >

Study on plasma protein binding rates of bufalin,cinobufagin and resibufogenin in different kinds of toad skin extracts

更新时间：2022-08-03

- Study on plasma protein binding rates of bufalin,cinobufagin and resibufogenin in different kinds of toad skin extracts
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 7, Pages: 81-84(2021)
- 作者机构：
  
  1.广州医药研究总院有限公司（广东　广州　510220）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2103056
  CLC：
扫描看全文
Jingjing SUN, Shan LING, Zhongfang HU, et al. Study on plasma protein binding rates of bufalin,cinobufagin and resibufogenin in different kinds of toad skin extracts. [J]. Shanghai Journal of Traditional Chinese Medicine 55(7):81-84(2021)
DOI：

Jingjing SUN, Shan LING, Zhongfang HU, et al. Study on plasma protein binding rates of bufalin,cinobufagin and resibufogenin in different kinds of toad skin extracts. [J]. Shanghai Journal of Traditional Chinese Medicine 55(7):81-84(2021) DOI： 10.16305/j.1007-1334.2021.2103056.

摘要

目的,2,研究不同蟾皮提取物中蟾毒灵、华蟾酥毒基和脂蟾毒配基的血浆蛋白结合率。,方法,2,采用平衡透析法，以高效液相色谱-串联质谱（LC-MS/MS）法测定，比较两种蟾皮提取物及不同浓度的蟾毒灵、华蟾酥毒基和脂蟾毒配基对照品溶液的血浆蛋白结合率。,结果,2,建立的LC-MS/MS法线性良好，精密度、准确度、回收率以及稳定性均符合生物样品分析要求。3种不同浓度蟾毒灵的血浆蛋白结合率分别为（75.03±1.97）%、（72.33±2.51）%、（71.80±1.53）%；蟾皮药材提取物1中的为（-16.67±1.58）%，蟾皮药材提取物2中的为（71.93±1.48）%。3种不同浓度的华蟾酥毒基血浆蛋白结合率分别为（52.33±4.13）%、（52.47±3.84）%、（47.60±2.46）%；蟾皮药材提取物1中的为（－51.23±3.53）%，蟾皮药材提取物2中的为（40.93±1.29）%。3种不同浓度的脂蟾毒配基血浆蛋白结合率分别为（91.27±1.09）%、（93.60±0.85）%、（88.13±0.76）%；蟾皮药材提取物1中的为（52.67±3.58）%，蟾皮药材提取物2中的为（80.13±2.38）%。,结论,2,蟾毒灵、华蟾酥毒基和脂蟾毒配基的血浆蛋白结合率跟药物浓度不呈依赖性，蟾皮药材提取物1中蟾毒灵、华蟾酥毒基和脂蟾毒配基的血浆蛋白结合率显著低于对照品蟾毒灵、华蟾酥毒基和脂蟾毒配基，而蟾皮药材提取物2的血浆蛋白结合率基本与对照品一致；提示蟾皮药材提取物1中混合物体系导致蟾毒灵、华蟾酥毒基和脂蟾毒配基的血浆蛋白结合率的改变。

Abstract

Objective,2,To study the plasma protein binding rates of bufalin, cinobufagin and resibufogenin in different kinds of toad skin extracts.,Methods,2,The plasma protein binding rates of bufalin, cinobufagin and resibufogenin in two extracts of toad skin and control solution with different concentrations of bufogenin, cinobufagin and cinobufagin were determined by equilibrium dialysis and LC-MS/MS method.,Results,2,The LC-MS/MS method showed good linearity, the accuracy, precision, recovery and stability of the analytes all met the methodology requirements. The plasma protein binding rates of bufalin in three different concentrations of control solution and in two kinds of toad skin extracts were (75.03±1.97) %, (72.33±2.51) %, (71.80±1.53) %, (-16.67±1.58) % and (71.93±1.48) %, respectively. The plasma protein binding rates of cinobufagin in three different concentrations of control solutionand in two kinds of toad skin extracts were (52.33±4.13) %, (52.47±3.84) %, (47.60±2.46) %, (-51.23±3.53) % and (40.93±1.29) %, respectively. The plasma protein binding rates of resibufogenin in three concentrations of control solution and in two kinds of toad skin extracts were (91.27±1.09) %, (93.60±0.85) %, (88.13±0.76) %, (52.67±3.58) % and (80.13±2.38) %, respectively.,Conclusions,2,The results show that the plasma protein binding rates of bufalin, cinobufagin and resibufogenin are not dependent on the drug concentration. The plasma protein binding rates of them in extract 1 are significantly lower than those of control solution, while the plasma protein binding rates of them in toad skin extract 2 are basically the same as those of the control solution. It is suggested the mixture system of toad skin extract 1 leads to the change of plasma protein binding rates of bufalin, cinobufagin and resibufogenin.

关键词

蟾皮提取物蟾毒灵华蟾酥毒基脂蟾毒配基血浆蛋白结合率平衡透析法中药研究

Keywords

toad skin extractsbufalincinobufaginresibufogeninprotein binding rateequilibrium dialysisresearch of traditional Chinese medicine

references

张英，邱鹰昆，刘珂，等. 中华大蟾蜍的研究进展[J]. 中草药，2006, 37(12): 1905-1908.

吴喜燕，高慧敏，王智民. 蟾蜍类药材化学成分研究进展[J]. 中国实验方剂学杂志，2010, 16(14): 207-214.

辛少鲲，司南，王宏洁，等. 蟾皮中亲水性成分的化学研究[J]. 中国中药杂志，2016, 41(20): 3767-3772.

刘颖，陈志强，陶蓓蕾，等. 蟾毒灵血浆蛋白结合率的测定[J]. 中国中药杂志，2009, 34(21): 2817-2820.

曹伟，陈彦，袁菱，等. 华蟾酥毒基和酯蟾毒配基与不同血浆蛋白结合率的测定[J]. 药学学报，2012, 47(9): 1200-1204.

李瑞光，陈历俊，姜铁民，等. 应用平衡透析法分析药物小分子与蛋白质相互作用[J]. 生物技术通报，2010(6): 80-84.

张弘，张晖芬，常会超，等. 齐墩果酸在人血浆蛋白和血清白蛋白中结合率的测定[J]. 药学学报，2011, 46(2): 243-246.

曾洋，张爱军，文筱. 干蟾皮的研究进展[J]. 中国医药科学，2011, 1(15): 29-31.

黄玉叶，宋霄宏. 蟾皮的提取和含量测定及临床应用研究进展[J]. 中华中医药学刊，2011, 29(7): 1636-1638.

ZHAO Q, LUAN X, ZHENG M, et al. Synergistic mechanisms of constituents in herbal extracts during intestinal absorption: focus on natural occurring nanoparticles[J]. Pharmaceutics, 2020, 12(2): 128.

Views

367

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Transcriptomics⁃based exploration on mechanism of action of cinobufagin in inhibiting the proliferation of hepatocellular carcinoma cells

Research progress on anti⁃hepatic cancer mechanism of Scutellariae Barbatae Herba

Research progress on antitumor mechanism of bufalin

Mechanism of cinobufagin inhibiting migration and invasion in human osteosarcoma cells

Effects of Sijunzi Decoction and Siwu Decoction on expression of EGF, EGF mRNA, VEGFA and VEGF mRNA in cultured human skin fibroblasts in vitro

Related Author

No data

Related Institution

Institute of Interdisciplinary Integrative Medicine Research， Shanghai University of Traditional Chinese Medicine

Yueyang Hospital of Integrated Traditional Chinese and Western Medicine， Shanghai University of Traditional Chinese Medicine

Department of Oncology， Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Shanghai Municipal Hospital of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine

Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University

⁰