Simultaneous determination of five constituents in Yinban Prescription by HPLC wavelength switching method

Jinghua QIAO; Yuanyuan LI; Mingda GU

doi:10.16305/j.1007-1334.2021.2103018

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Simultaneous determination of five constituents in Yinban Prescription by HPLC wavelength switching method

更新时间：2022-08-03

- Simultaneous determination of five constituents in Yinban Prescription by HPLC wavelength switching method
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 8, Pages: 75-79(2021)
- 作者机构：
  
  1.上海市儿童医院，上海交通大学附属儿童医院中医科（上海　200062）
  2.杭州医学院药学院（浙江　杭州　310053）
  3.上海中医药大学附属曙光医院儿科（上海　200032）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2103018
  CLC：
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Jinghua QIAO, Yuanyuan LI, Mingda GU. Simultaneous determination of five constituents in Yinban Prescription by HPLC wavelength switching method. [J]. Shanghai Journal of Traditional Chinese Medicine 55(8):75-79(2021)
DOI：

Jinghua QIAO, Yuanyuan LI, Mingda GU. Simultaneous determination of five constituents in Yinban Prescription by HPLC wavelength switching method. [J]. Shanghai Journal of Traditional Chinese Medicine 55(8):75-79(2021) DOI： 10.16305/j.1007-1334.2021.2103018.

摘要

目的,2,建立高效液相色谱（HPLC）波长切换法同时测定“银板方”中（R,S）-告依春、绿原酸、芍药内酯苷、连翘酯苷A和次野鸢尾黄素含量的方法。,方法,2,采用Agilent InfinityLab Poroshell 120 SB-C18 (3.0 mm×150 mm, 2.7 μm)色谱柱，0.1%磷酸乙腈-0.1%磷酸水为流动相，梯度洗脱，流速为0.8 mL/min，柱温 35 ℃；波长切换方式0～4 min为240 nm，4～6.5 min为325 nm，6.5～9 min为230 nm，9～21 min为328 nm，21～24 min为265 nm。,结果,2,（R,S）-告依春、绿原酸、芍药内酯苷、连翘酯苷A、次野鸢尾黄素分别在0.145～18.560（,r,＝1）、3.566～228.200（,r,＝1）、2.281～292.000（,r,＝1）、6.312～808.000（,r,＝1）、0.238～30.480（,r,＝1）g范围内与峰面积线性关系良好，平均回收率分别为97.86%、102.09%、99.06%、98.63%、101.51%，,RSD,分别为1.90%、1.43%、1.62%、1.40%、1.94%（,n,=9）。,结论,2,HPLC波长切换法准确、可靠、重复性好，可用于“银板方”的质量评价。

Abstract

Objective,2,To develop an HPLC wavelength switching method for determining the contents of (R,S) -goitrin, chlorogenic acid, albiflorin, forsythoside A, irisflorentin in Yinban Prescription.,Methods,2,The separation was performed on an Agilent InfinityLab Poroshell 120 SB-C18 (3.0 mm×150 mm, 2.7 μm) at 35 ℃ using a gradient elution with 0.1% phosphoric acid acetonitrile and 0.1% phosphoric acid water. The flow rate was 0.8 mL/min and the detection wavelength was set at 240 nm within 0-4 min, 325 nm within 4-6.5 min, 230 nm within 6.5-9 min, 328 nm within 9-21 min, 265 nm within 21-24 min.,Results,2,(R,S)-goitrin, chlorogenic acid, albiflorin, forsythoside A, risflorentin had a good linear relationship with the peak area in the range of 0.145-18.560 (,r,=1) , 3.566-228.200 (,r,=1) , 2.281-292.000 (,r,=1) , 6.312-808.000 (,r,=1) , 0.238-30.480 (,r,=1) g, respectively. The average recoveries were 97.86%, 102.09%, 99.06%, 98.63%, 101.51%, with ,RSD, 1.90%, 1.43%, 1.62%, 1.40%, 1.94% (,n,=9), respectively.,Conclusion,2,The HPLC wavelength switching method is accurate, reliable and reproducible for quality evaluation of Yinban Prescription.

关键词

银板方（RS）-告依春绿原酸芍药内酯苷连翘酯苷A次野鸢尾黄素高效液相色谱法含量测定

Keywords

Yinban Prescription(RS)-goitrinchlorogenic acidalbiflorinforsythoside AirisflorentinHPLCcontent determination

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