Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model

Shuai AN; Rongchuang BAI; Gege LI; Long ZHU; Yingxin YANG

doi:10.16305/j.1007-1334.2021.2101075

您当前的位置：

首页 >

文章列表页 >

Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model

更新时间：2022-08-03

- Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 7, Pages: 64-67(2021)
- 作者机构：
  
  1.辽宁中医药大学（辽宁　沈阳　110847）
  2.沈阳维康医院（辽宁　沈阳　110021）
  3.中国人民解放军北部战区总医院（辽宁　沈阳　110034）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2101075
  CLC：
扫描看全文
Shuai AN, Rongchuang BAI, Gege LI, et al. Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model. [J]. Shanghai Journal of Traditional Chinese Medicine 55(7):64-67(2021)
DOI：

Shuai AN, Rongchuang BAI, Gege LI, et al. Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model. [J]. Shanghai Journal of Traditional Chinese Medicine 55(7):64-67(2021) DOI： 10.16305/j.1007-1334.2021.2101075.

摘要

目的,2,观察茯苓丸加减方对兔膝骨关节炎（KOA）模型MMP-3、MMP-13及TIMP-1表达的影响。,方法,2,将日本大耳白兔随机分为正常组、模型组、双氯芬酸钠组和茯苓丸加减方组，每组6只；除正常组外，各组均采用改良Hulth法对其左后膝关节进行手术造模；造模成功后对各组大耳白兔进行4周的干预治疗，治疗结束后进行病理观察，同时采用ELISA 法对各组大耳白兔膝关节液内MMP-3、MMP-13和TIMP-1水平进行测定。,结果,2,模型组MMP-3、MMP-13和TIMP-1含量较正常组均明显升高（,P,<,0.01）；与模型组比较，双氯芬酸钠组和茯苓丸加减方组均明显下降（,P,<,0.01）。,结论,2,MMP-3、MMP-13和TIMP-1与KOA发病关系密切，茯苓丸加减方可能是通过抑制其病理性升高对KOA的发病起到治疗作用。

Abstract

Objective,2,To observe the effect of Modified Fulingwan Decoction on the expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model.,Methods,2,Twenty-four Japan big ear white rabbits were randomly divided into normal group, model group, diclofenac sodium group and Modified Fulingwan Decoction group, 6 rabbits in each group. Except for the normal group, the modified Hulth method was used on the left posterior knee joint in other groups. After successful modeling, each group was intervened for four weeks. After the treatment cycle, and pathological observation was performed after the treatment. At the same time, ELISA was used to detect the levels of MMP-3, MMP-13 and TIMP-1 in the knee joint fluid of each groups’ subjects.,Results,2,The contents of MMP-3, MMP-13 and TIMP-1 in model group were significantly increased than those in the normal group (,P,<,0.01) . The contents in diclofenac sodium group and modified Fulingwan group were significantly decreased than those in model group (,P,<,0.01) .,Conclusions,2,MMP-3, MMP-13 and TIMP-1 are closely related to the pathogenesis of KOA. Modified Fuling Decoction may have a therapeutic effect on the pathogenesis of KOA by inhibiting their pathological increase.

关键词

茯苓丸加减方膝骨性关节炎MMP-3MMP-13TIMP-1

Keywords

Modified Fulingwan Decoctionknee osteoarthritisMMP-3MMP-13TIMP-1

references

JANSEN M P, MASTBERGEN S C, VAN HEERWAARDEN R J, et al. Knee joint distraction in regular care for treatment of knee osteoarthritis: A comparison with clinical trial data[J/OL]. PLoS One, 2020[2020-11-18]. https://pubmed.ncbi.nlm.nih.gov/31968005/https://pubmed.ncbi.nlm.nih.gov/31968005/.

KAY A D，DIXON J，BLIGH L D，et al. The external validity of a novel contract-relax stretching technique on knee flexor range of motion[J]. Scand J Med Sci Sports, 2020, 30(1): 74-82.

李万军，马新焕，王建良. 半夏的药理作用[J]. 西部中医药，2012, 25(9): 129-131.

林竹霞，谢晋，张俊婷，等. 牛膝“下行”功效考辨[J]. 中药材，2018, 41(11): 2709-2711.

朱小芳，管咏梅，刘莉，等. 乳香、没药药对的研究进展[J]. 江西中医药，2016, 47(12): 72-74.

杨军，娄德全，周振东，等. 基质金属蛋白酶和胶原在创伤关节软骨组织中的表达[J]. 中国组织工程研究，2011, 15(20): 3636-3640.

PELLETIER J P, MARTEL-PELLETIER J, ABRAMSON S B. Osteoarthritis, an inflammatory disease: potential implication for the selection of new therapeutic targets[J]. Arthritis Rheum, 2001, 44(6): 1237-1247.

艾倩，杨会明. 骨性关节炎关节液中白细胞介素6和基质金属蛋白酶3的表达[J]. 中国组织工程研究，2015, 19(33): 5259-5263.

WOESSNER J F J. Matrix metalloproteinases and their inhibitors in connective tissue remodeling[J]. FASEB J,1991, 5(8): 2145-2154.

KAYANI B，KONAN S，AHMED S S，et al. The effect of anterior cruciate ligament resection on knee biomechanics[J]. Bone Joint J, 2020, 102-B(4): 442-448.

DÖRR S, LECHTENBÖHMER N, RAU R, et al. Association of a specific haplotype across the genes MMP-1 and MMP-3 with radiographic joint destruction in rheumatoid arthritis[J]. Arthritis Res Ther, 2004, 6(3): R199-R207.

Views

284

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Synergistic effects of Chaihu (Bupleuri Radix) on TIMP-1 and TGF-β1 in rats with pancreatic fibrosis

Effects of modified kinesio taping of integrated Chinese and western medicine on knee osteoarthritis

Related Author

No data

Related Institution

Department of General Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Institute of Chinese Medical Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Clinical Medical College,Shanghai First People’s Hospital Affiliated to Nanjing Medical University

Department of Rehabilitation,Shanghai First People’s Hospital

Sanda University

⁰