Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion

Mingzhe HU; Xiangling LI; Tao YANG; Minyan WANG; Xiaolong WANG; Junjie GAO

doi:10.16305/j.1007-1334.2021.2101068

您当前的位置：

首页 >

文章列表页 >

Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion

更新时间：2022-08-03

- Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 6, Pages: 61-66(2021)
- 作者机构：
  
  1.上海中医药大学附属曙光医院心内科（上海　201203）
  2.上海中医药大学附属曙光医院心血管病研究所（上海　201203）
  3.上海中医药大学附属曙光医院病理科（上海　201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2101068
  CLC：
扫描看全文
Mingzhe HU, Xiangling LI, Tao YANG, et al. Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion. [J]. Shanghai Journal of Traditional Chinese Medicine 55(6):61-66(2021)
DOI：

Mingzhe HU, Xiangling LI, Tao YANG, et al. Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion. [J]. Shanghai Journal of Traditional Chinese Medicine 55(6):61-66(2021) DOI： 10.16305/j.1007-1334.2021.2101068.

摘要

目的,2,观察丹参饮预处理对心肌缺血/再灌注损伤（MI/RI）大鼠心肌梗死面积、心功能和炎症因子的影响。,方法,2,超高效液相色谱-高分辨质谱联用分析法分析丹参饮的化学成分；将Wistar大鼠随机分为假手术组、模型组、丹参饮低剂量预处理I/R组、丹参饮中剂量预处理I/R组、丹参饮高剂量预处理I/R组；给药1周后，采用大鼠冠状动脉前降支结扎30 min/复流24 h的方法建立心肌缺血/再灌注模型；2,3,5-氯化三苯基四氮唑染色观察心肌梗死面积，ImageJ软件计算心肌梗死面积；使用心脏彩色多普勒仪观察各组大鼠左室射血分数（LVEF）和左室缩短率（LVFS）；采用ELISA法测定各组大鼠血清白介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达。,结果,2,①丹参饮的主要化学成分为丹酚酸B、丹参酮ⅡA、槲皮素、槲皮苷。②与模型组比较，丹参饮各剂量组大鼠心肌梗死面积均有下降（,P,<,0.05）。③与假手术组比较，模型组大鼠的LVEF和LVFS均明显降低（,P,<,0.01）；与模型组比较，丹参饮各剂量预处理I/R组大鼠的LVEF和LVFS均有不同程度改善（,P,<,0.05，,P,<,0.01）。④与假手术组比较，模型组大鼠血清中IL-6和TNF-α含量均有增加（,P,<,0.01）；与模型组比较，丹参饮各剂量预处理I/R组大鼠血清IL-6水平均明显下降（,P,<,0.01），丹参饮低、中剂量预处理I/R组TNF-α水平亦均有下降（,P,<,0.05）。,结论,2,丹参饮预处理具有减少MI/RI大鼠心肌梗死面积、改善心功能的作用，其机制可能与减少缺血再灌注时血清炎症因子的表达相关。

Abstract

Objective,2,To observe the effect of Danshen Decoction (DSY) pretreatment on infarct size, cardiac function and inflammatory cytokines in serum in rats with myocardial ischemia/reperfusion injury (MI/RI).,Methods,2,Chemical components of DSY were analyzed by ultra-high performance liquid chromatography/ultra-resolution mass spectrometry. Thirty Wistar rats were randomly divided into five groups: sham operation group, ischemia/reperfusion (I/R) model group, DSY (low-dose, medium-dose and high-dose) pretreatment I/R groups, with 6 rats in each group. After 1 week of continuous gavage, the rat model of myocardial ischemia/reperfusion (I/R) was established by ligation of the anterior descending coronary artery for 30 min/reflow for 24h. TTC staining was used to observe the infarct size, which was measured by ImageJ software. Left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) were observed by Cardiac Color Ultrasound Doppler. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in serum of each rat group were determined by ELISA.,Results,2,①The main chemical components of DSY were salvianolic acid B, tanshinone IIA, quercetin and quercitrin. ②Compared with the model group, the size of myocardial infarction was decreased in DSY (low-dose, medium-dose and high dose) preconditioning groups (,P,<,0.05). ③Compared with sham operation group, LVEF and LVFS in I/R model group were significantly decreased (,P,<,0.05,P,<,0.01). Compared with I/R model group, the LVEF and LVFS of rats in DSY (low-dose, medium-dose and high dose) preconditioning groups were improved (,P,<,0.05). ④Compared with the sham operation group, the levels of IL-6 and TNF-α in serum in I/R model group were increased (,P,<,0.01). Compared with I/R model group, the levels of IL-6 in serum in DSY (low-dose, medium-dose and high dose) pretreatment groups were decreased (,P,<,0.01). The levels of TNF-α in serum in DSY (low-dose and medium-dose) pretreatment groups were also decreased (,P,<,0.05). There was no significant difference of TNF-α expression between the high-dose group and the model group (,P,>,0.05).,Conclusion,2,DSY pretreatment has the effect of reducing infarct size, improving cardiac function of MI/RI rats, whose mechanism may be related to the reduction of the expression of inflammatory cytokines in serum of MI/RI rats.

关键词

丹参饮心肌缺血/再灌注损伤心功能炎症因子心肌保护

Keywords

Danshen Decoctionmyocardial ischemia/reperfusion injurycardiac functioninflammatory cytokinescardioprotection

references

TIAN L, CAO W J, YUE R J, et al. Pretreatment with Tilianin improves mitochondrial energy metabolism and oxidative stress in rats with myocardial ischemia/reperfusion injury via AMPK/SIRT1/PGC-1 alpha signaling pathway[J]. J Pharmacol Sci, 2019, 139(4): 352-360.

YELLON D M, HAUSENLOY D J. Myocardial reperfusion injury[J]. N Engl J Med, 2007, 357(11): 1121-1135.

KELLER M, MIRAKAJ V, KOEPPEN M, et al. Neuronal guidance proteins in cardiovascular inflammation[J]. Basic Res Cardiol, 2021, 116(1): 6.

李平，金哈布日，张青山，等. 蒙药黄连-4汤对心肌缺血再灌注损伤SD大鼠炎症因子TNF-α、IL-6的影响[J]. 中西医结合心血管病电子杂志，2017, 5(33): 124-125.

胡益森，熊劲节，徐戈. 替米沙坦对大鼠心肌缺血再灌注诱导的心肌炎症反应的影响[J]. 内科，2019, 14(3): 253-256.

朱文叶，王肖龙，高俊杰，等. 中医药在防治急性心肌缺血/再灌注损伤中的研究进展[J]. 中国医药导报，2015, 12(29): 31-34.

高俊杰，朱文叶，李益萍，等. 丹参饮预处理对心肌缺血/再灌注损伤大鼠的心肌保护作用研究[J]. 中西医结合心脑血管病杂志，2019, 17(1): 50-54.

JIN P, LI L H, SHI Y, et al. Salidroside inhibits apoptosis and autophagy of cardiomyocyte by regulation of circular RNA hsa_circ_0000064 in cardiac ischemia-reperfusion injury[J/OL]. Gene, 2021[2021-01-11]. https://pubmed.ncbi.nlm.nih.gov/32858179/https://pubmed.ncbi.nlm.nih.gov/32858179/.

ROSANO G M, FINI M, CAMINITI G, et al. Cardiac metabolism in myocardial ischemia[J]. Curr Pharm Des, 2008,14(25): 2551-2562.

KRENNING B J, DER HEIDEN K V, DUNCKER D J, et al. Nuclear imaging post-infarction inflammation in ischemic cardiac diseases-new radiotracers for potential clinical applications[J/OL]. Curr Radiopharm, 2020[2020-12-11]. https://pubmed.ncbi.nlm.nih.gov/33045975/https://pubmed.ncbi.nlm.nih.gov/33045975/.

XUE Y, FU W W, LIU Y Z, et al. Ginsenoside Rb2 alleviates myocardial ischemia/reperfusion injury in rats through SIRT1 activation[J]. J Food Sci, 2020, 85(11): 4039-4049.

NING K, JIANG L, HU T, et al. ATP-sensitive potassium channels mediate the cardioprotective effect of Panax notoginseng saponins against myocardial ischaemia-reperfusion injury and inflammatory reaction[J/OL]. Biomed Res Int, 2020[2020-12-14]. https://pubmed.ncbi.nlm.nih.gov/33134375/https://pubmed.ncbi.nlm.nih.gov/33134375/.

MIAO Y, DING Z C, ZOU Z M, et al. Inhibition of MyD88 by a novel inhibitor reverses two-thirds of the infarct area in myocardial ischemia and reperfusion injury[J]. Am J Transl Res, 2020, 12(9): 5151-5169.

PENG K, LIU H, YAN B, et al. Inhibition of cathepsin S attenuates myocardial ischemia/reperfusion injury by suppressing inflammation and apoptosis[J]. J Cell Physiol, 2021, 236(2): 1309-1320.

YANG W W, LAI Q, ZHANG L, et al. Mechanisms dissection of the combination GRS derived from ShengMai preparations for the treatment of myocardial ischemia/reperfusion injury[J/OL]. J Ethnopharmacol, 2021[2021-01-11]. https://pubmed.ncbi.nlm.nih.gov/32946961/https://pubmed.ncbi.nlm.nih.gov/32946961/.

孙正骥，吴露，张伟. 基于炎症反应探讨活血化瘀法防治动脉粥样硬化的策略[J]. 中西医结合心脑血管病杂志，2020, 18(18): 3012-3016.

耿彬，苗华为，王思洲，等. 活血化瘀养心通络方联合替格瑞洛治疗冠心病经皮冠状动脉介入术术后心绞痛的临床疗效及对血管内皮功能及炎症因子的影响[J]. 中华中医药学刊，2020, 38(11): 33-37.

肖新怀，徐米清，方燕龄，等. 丹红注射剂在冠状动脉粥样硬化症患者中的作用研究[J]. 中国药物与临床，2019, 19(2): 245-246.

Views

539

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical effect of acupuncture combined with Chinese medicine in treatment of polycystic ovary syndrome and its influence on serum IL⁃6

Experimental study on effect of Luqi Granule on cardiac function and serum endotoxin level in rats with acute myocardial infarction

Effects of artemisinin on cardiac function and fibrosis in diabetic cardiomyopathy rats

The study of “Quyu Jiedu Yiqi Decoction” on levels of plasma calcitonin proinsulin and diamine oxidase in patients with sepsis

Related Author

No data

Related Institution

Department of Gynecology， Shanghai Municipal Hospital of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine

Department of Acupuncture and Moxibustion， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Gynecology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Cardiology of Internal Medicine, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine

Institute of Cardiology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine

⁰