Mechanism of reversal of adriamycin-resistant hepatocellular carcinoma with ethanol extracts of Oldenlandia diffusa roxb and Scutellariae barbatae herba

Yuncui PAN; Qian WANG; Aiyan HU; Yi XU; Xiaoyu NIE; Xin ZHANG; Shoumei WANG; Shuhui ZHANG

doi:10.16305/j.1007-1334.2021.2101014

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Mechanism of reversal of adriamycin-resistant hepatocellular carcinoma with ethanol extracts of Oldenlandia diffusa roxb and Scutellariae barbatae herba

更新时间：2022-08-03

- Mechanism of reversal of adriamycin-resistant hepatocellular carcinoma with ethanol extracts of Oldenlandia diffusa roxb and Scutellariae barbatae herba
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 10, Pages: 52-57(2021)
- 作者机构：
  
  1.上海中医药大学附属岳阳中西医结合医院病理科（上海　200437）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2101014
  CLC：
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Yuncui PAN, Qian WANG, Aiyan HU, et al. Mechanism of reversal of adriamycin-resistant hepatocellular carcinoma with ethanol extracts of Oldenlandia diffusa roxb and Scutellariae barbatae herba. [J]. Shanghai Journal of Traditional Chinese Medicine 55(10):52-57(2021)
DOI：

Yuncui PAN, Qian WANG, Aiyan HU, et al. Mechanism of reversal of adriamycin-resistant hepatocellular carcinoma with ethanol extracts of Oldenlandia diffusa roxb and Scutellariae barbatae herba. [J]. Shanghai Journal of Traditional Chinese Medicine 55(10):52-57(2021) DOI： 10.16305/j.1007-1334.2021.2101014.

摘要

目的,2,研究白花蛇舌草（Od）和半枝莲（Sb）乙醇提取物对耐阿霉素肝细胞癌（HepG2/ADM）增殖和凋亡的作用。,方法,2,①体外实验：采用CCK-8法检测不同浓度的Od-Sb对HepG2/ADM的影响；克隆形成实验检测Od-Sb对HepG2/ADM克隆形成能力的影响；流式细胞术检测Od-Sb对HepG2/ADM凋亡的影响；Western blot检测Od-Sb对HepG2/ADM中Ki-67、Bcl-2蛋白表达的影响。②体内实验：以裸鼠HepG2/ADM皮下移植瘤为模型，观察Od-Sb对瘤体的作用，并采用免疫组化法检测Ki-67、Bcl-2蛋白的表达。,结果,2,①体外实验显示，与对照组比较，Od-Sb可以抑制HepG2/ADM细胞的增殖，抑制HepG2/ADM细胞的克隆形成，促进其凋亡，并下调Ki-67、Bcl-2蛋白的表达（,P,<,0.05）。②体内实验显示，与对照组比较，Od-Sb可以抑制裸鼠HepG2/ADM皮下移植瘤瘤体的生长，下调Ki-67、Bcl-2蛋白的表达（,P,<,0.05，,P,<,0.001）。,结论,2,Od-Sb可下调HepG2/ADM 中Ki-67、Bcl-2蛋白的表达，抑制HepG2/ADM的生长，并促进其凋亡；Od-Sb通过增殖-凋亡调控机制部分逆转HepG2/ADM的耐药。

Abstract

Objective,2,To study the effects of Oldenlandia diffusa roxb and Scutellariae barbatae herba (Od-Sb) on the proliferation and apoptosis of HepG2/ADM.,Methods,2,①In vitro experiment: the effect of different concentrations of Od-Sb on the survival rate of HepG2/ADM was detected by CCK8 assay; the effect of Od-Sb on HepG2/ADM clonal formation ability was detected by cloning formation experiment; the effect of Od-Sb on the apoptosis of HepG2/ADM was detected by flow cytometry; the effect of Od-Sb on the protein expression of Ki-67 and Bcl-2 was detected by Western blot. ②In vivo experiment: the subcutaneous transplanted tumor of HepG2/ADM in nude mice was used as a model to observe the inhibitory effect of Od-Sb on the tumor, and the proteins expression of Ki-67 and Bcl-2 was detected by immunohistochemistry.,Results,2,①In vitro experiments, it was showed that compared with the control group, Od-Sb could inhibit the proliferation of HepG2/ADM cells, inhibit the formation of HepG2/ADM cells, promote its apoptosis, and down-regulated the expression of Ki-67 and Bcl-2 proteins (,P,<,0.05) . ② In vivo experiments showed that compared with the control group, Od-Sb inhibited the growth of HepG2/ADM subcutaneous transplanted tumor body, and down-regulated the expression of Ki-67 and Bcl-2 proteins (,P,<,0.05,P,<,0.001) .,Conclusion,2,Od-Sb can down-regulate the expression of Ki-67 and Bcl-2 proteins, inhibit the growth of HepG2/ADM and promote its apoptosis. Od-Sb can partially reverse the resistance of HepG2/ADM through the mechanism of proliferation-apoptosis regulation.

关键词

白花蛇舌草半枝莲肝细胞癌阿霉素耐药模型小鼠中药研究

Keywords

Oldenlandia diffusa roxbScutellariae barbata herbahepatocellular carcinomaadrimycindrug resistancemodel micetraditional Chinese herbal medicine research

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