Objective,2,To establish a new method for determination of seven components (allantoin, hesperidin, narirutin, isosinensetin, nobiletin, 3, 5, 6, 7, 8, 3’, 4’-heptamethoxyflavone, tangeretin) in Jianpi Prescription by ultra performance liquid chromatography (UPLC) with diode array detector (DAD) and to carry out its application research.,Methods,2,Agilent 1290 series ultra high performance liquid chromatography was used to detect the seven components by UV multi-wavelength detection. The detection wavelength of allantoin was set at 224 nm, with narirutin and hesperidin at 284 nm, isosinensetin at 342 nm, and nobiletin, 3, 5, 6, 7, 8, 3’, 4’-heptamethoxyflavone and tangeretin at 333 nm. The method was used to analyze the contents of seven components in Jianpi Prescription extracts prepared by different extraction methods.,Results,2,In the appropriate concentration range for all above seven components, the concentrations showed quite good linear relationship with the peak area (,r,>,0.999 3).All indexes of the methodological investigations including precision, repeatability, stability and accuracy met the requirements. The contents of seven components in Jianpi Prescription extracts prepared by various of extraction methods were significantly different. The concentration of allantoin in water decoction of Jianpi prescription was the highest, while the concentrations of hesperidin, narirutin, isosinensetin, nobiletin, 3, 5, 6, 7, 8, 3’, 4’-heptamethoxyflavone and tangeretin in ethanol reflux liquid were higher than those in water decoction of Jianpi Prescription. It was suggested that in the extraction and purification process of Jianpi Prescription, the rationality of the method should be evaluated from the point of view of multi-component content analysis.,Conclusion,2,The established method based on UPLC-DAD in this study is stable and reliable, and can provide reference for its process research and quality control.