Effect of Bushen Huoxue Formula on expression of VEGF/PI3K/Akt pathway in decidua of mice with recurrent abortion

Jie ZHANG; Shuang NI; Ji LI

doi:10.16305/j.1007-1334.2021.2008069

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Effect of Bushen Huoxue Formula on expression of VEGF/PI3K/Akt pathway in decidua of mice with recurrent abortion

更新时间：2022-08-03

- Effect of Bushen Huoxue Formula on expression of VEGF/PI3K/Akt pathway in decidua of mice with recurrent abortion
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 2, Pages: 82-89(2021)
- 作者机构：
  
  1.上海中医药大学附属龙华医院临床研究中心（上海　200032）
  2.上海中医药大学附属龙华医院妇科（上海　200032）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2008069
  CLC：
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Jie ZHANG, Shuang NI, Ji LI. Effect of Bushen Huoxue Formula on expression of VEGF/PI3K/Akt pathway in decidua of mice with recurrent abortion. [J]. Shanghai Journal of Traditional Chinese Medicine 55(2):82-89(2021)
DOI：

Jie ZHANG, Shuang NI, Ji LI. Effect of Bushen Huoxue Formula on expression of VEGF/PI3K/Akt pathway in decidua of mice with recurrent abortion. [J]. Shanghai Journal of Traditional Chinese Medicine 55(2):82-89(2021) DOI： 10.16305/j.1007-1334.2021.2008069.

摘要

目的,2,观察补肾活血方对复发性流产小鼠蜕膜组织VEGFA、VEGFR2及对PI3k/Akt信号转导通路的影响，探讨其治疗复发性流产的可能作用机制。,方法,2,建立BALB/c×CBA/J 正常妊娠小鼠模型及DBA/2×CBA/J复发性流产小鼠模型，实验随机分为正常组、模型组、黄体酮组（3.33 mg/kg）、补肾活血方低剂量组（10.47 g/kg）、补肾活血方中剂量组（20.93 g/kg）、补肾活血方高剂量组（41.87 g/kg），各组给予相应药物灌胃2周。观察各组不同浓度药物干预后CBA/J小鼠的胚胎丢失率；采用免疫组化检测小鼠蜕膜组织VEGFA、VEGFR2蛋白的表达；采用Western blot检测小鼠蜕膜组织VEGFA、VEGFR2、PI3K、Akt、p-Akt蛋白的表达。,结果,2,①与模型组比较，黄体酮组和补肾活血方低、中、高剂量组小鼠胚胎丢失率均明显下降（,P,<,0.05）。②免疫组化结果表明，与模型组比较，黄体酮组和补肾活血方中、高剂量组VEGFA蛋白阳性表达增强，黄体酮组和补肾活血方低、中、高剂量组VEGFR2蛋白阳性表达增强（,P,<,0.05）；与补肾活血方低剂量组比较，补肾活血方中、高剂量组VEGFA蛋白阳性表达增强（,P,<,0.05）。③Western blot结果表明，与模型组比较，黄体酮组和补肾活血方低、中、高剂量组VEGFA、VEGFR2、PI3K、p-Akt蛋白表达水平明显升高（,P,<,0.05）；与补肾活血方低、中剂量组比较，补肾活血方高剂量组VEGFA、VEGFR2、PI3K、p-Akt蛋白表达水平明显升高（,P,<,0.05）。,结论,2,补肾活血方能上调复发性流产模型小鼠蜕膜组织VEGFA及其受体VEGFR2的表达，其机制可能与激活PI3K/Akt信号通路相关。

Abstract

Objective,2,To observe the effect of Bushen Huoxue Formula on VEGFA, VEGFR2 and PI3K/Akt signal transduction pathway in decidua of recurrent abortion mice, and to explore the possible mechanism of Bushen Huoxue Formula in treating recurrent abortion.,Methods,2,BALB/c × CBA/J normal pregnant mice model and DBA/2 × CBA/J recurrent abortion mice model were established. The mice were randomly divided into normal group, model group, progesterone group (3.33 mg/kg), and Bushen Huoxue Formula low-dose (10.47 g/kg), medium-dose (20.93 g/kg) and high-dose (41.87 g/kg) groups. Each group was given corresponding drugs by gavage for 2 weeks. The embryo loss rate of CBA/J mice after different concentrations of drug intervention was observed; the expressions of VEGFA and VEGFR2 proteins in decidua were detected by immunohistochemistry; the expressions of VEGFA, VEGFR2, PI3K, Akt, p-Akt proteins in decidua were detected by Western blot.,Results,2,①Compared with the model group, the embryo loss rates of the progesterone group and the low-dose, medium-dose and high-dose groups of Bushen Huoxue Formula were significantly decreased (,P,<,0.05). ②The results of immunohistochemistry showed that: compared with the model group, the positive expression of VEGFA protein was increased in the progesterone group, and the medium-dose and high-dose groups of Bushen Huoxue Formula; the positive expression of VEGFR2 protein was increased in the progesterone group and the low-dose, medium-dose and high-dose groups of Bushen Huoxue Formula (,P,<,0.05); compared with the low-dose group of Bushen Huoxue Formula, the positive expression of VEGFA protein in the medium-dose and high-dose groups of Bushen Huoxue Formula was increased (,P,<,0.05). ③Western blot results showed that: compared with the model group, the expressions of VEGFA, VEGFR2, PI3K, p-Akt proteins in the progesterone group, and the low-dose, medium-dose and high-dose groups of Bushen Huoxue Formula were significantly increased (,P,<,0.05); compared with low-dose and medium-dose groups of Bushen Huoxue Formula, the protein expressions of VEGFA, VEGFR2, PI3K and p-Akt in the high-dose group of Bushen Huoxue Formula were significantly increased (,P,<,0.05).,Conclusion,2,Bushen Huoxue Formula can up-regulate the expression of VEGFA and VEGFR2 in decidua of recurrent abortion model mice, and the mechanism may be related to the activation of PI3K/Akt signaling pathway.

关键词

补肾活血方复发性流产小鼠蜕膜组织VEGFPI3K/Akt

Keywords

Bushen Huoxue Formularecurrent abortionmicedecidual tissueVEGFPI3K/Akt

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