Network pharmacological study on mechanism of Tenghuang Jiangu Tablet in the treatment of knee osteoarthritis

Dezhong KONG; Liang OU; Liyue GUO; Weichen HUANG; Xin CAI; Xingqian FU

doi:10.16305/j.1007-1334.2021.2008031

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Network pharmacological study on mechanism of Tenghuang Jiangu Tablet in the treatment of knee osteoarthritis

更新时间：2022-08-03

- Network pharmacological study on mechanism of Tenghuang Jiangu Tablet in the treatment of knee osteoarthritis
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 8, Pages: 4-12(2021)
- 作者机构：
  
  1.贵州中医药大学骨伤学院（贵州　贵阳　550025）
  2.贵州中医药大学第二附属医院骨科（贵州　贵阳　550003）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2008031
  CLC：
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Dezhong KONG, Liang OU, Liyue GUO, et al. Network pharmacological study on mechanism of Tenghuang Jiangu Tablet in the treatment of knee osteoarthritis. [J]. Shanghai Journal of Traditional Chinese Medicine 55(8):4-12(2021)
DOI：

Dezhong KONG, Liang OU, Liyue GUO, et al. Network pharmacological study on mechanism of Tenghuang Jiangu Tablet in the treatment of knee osteoarthritis. [J]. Shanghai Journal of Traditional Chinese Medicine 55(8):4-12(2021) DOI： 10.16305/j.1007-1334.2021.2008031.

摘要

目的,2,寻找藤黄健骨片作用于膝骨关节炎的主要作用靶点和主要有效成分。,方法,2,通过中药系统药理学数据库与分析平台（TCMSP）检索藤黄健骨片中所包含的化学成分和作用靶点，通过蛋白质序列数据库（UniProt KB）网络平台等8个数据库挖掘藤黄健骨片与膝骨关节炎相关的作用靶点，采用基因本体论（GO）分析、京都基因与基因组百科全书（KEGG）富集通路分析藤黄健骨片治疗膝骨关节炎的作用机制。,结果,2,从藤黄健骨片中筛选出柚皮素、甘草查尔酮A、芒柄花黄素、芦荟大黄素、脱水淫羊藿素、毛蕊异黄酮、8-甲雷杜辛等85种有效活性成分，作用于丝氨酸/苏氨酸蛋白激酶（AKT1）、丝裂原活化蛋白激酶3（MAPK3）、胱天蛋白酶3（CASP3）、信号传导与转录激活因子3（STAT3）、表皮生长因子受体（EGFR）、血管内皮生长因子（VEGFA）、表皮细胞生长因子（EGF）等121个膝骨关节炎相关的靶蛋白，参与脂多糖反应、细菌源性分子细胞反应、调控凋亡信号通路、调控细胞因子产生、应答氧化应激反应等生物学过程，调控晚期糖基化终产物及其受体（AGE-RAGE）信号通路、C型凝集素受体信号通路、肿瘤坏死因子（TNF）信号通路、白介素-17（IL-17）等信号通路。分子对接结果显示，甘草查尔酮A、柚皮素分别与蛋白靶点AKT1、白介素-6（IL-6）高效结合。,结论,2,藤黄健骨片通过多成分、多靶点、多信号通路，对膝骨关节炎发挥治疗作用。

Abstract

Objective,2,To explore the targets and components of Tenghuang Jiangu Tablet (THJGT) in treating knee osteoarthritis.,Methods,2,The active ingredients and targets of THJGT were collected and identified from TCMSP database. The targets of THJGT in treating knee osteoarthritis were mined by 8 databases including UniProt KB network platform. The enrichment analysis of gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) were used to analyze the mechanism of THJGT in the treatment of knee osteoarthritis.,Results,2,A total of 85 effective ingredients, such as naringin, licorice chalcone A, mangiferin, aloe emodin, dehydrated icariin, stamen isoflavone, 8-methaduraxine, etc., were obtained from THJGT, which acted on a total of 121 target proteins including AKT1, MAPK3, CASP3, STAT3, EGFR, VEGFA, EGF, etc. THJGT affected the lipopolysaccharide response, bacterial molecular cell response, the regulation of apoptosis signal pathway, the regulation of cytokine production, the response to oxidative stress and other biological processes, and regulated the AGE-RAGE signal pathway, C-type lectin receptor signal pathway, TNF signal pathway and IL-17 signal pathway. The results of molecular docking showed that the two active components, glycyrrhizin-A and naringin, could effectively bind to the protein target AKT1 and IL-6 respectively.,Conclusion,2,THJGT could play a role in treating knee osteoarthritis through multiple active ingredients, multiple targets and multiple pathways.

关键词

膝骨关节炎藤黄健骨片网络药理学信号通路分子对接

Keywords

knee osteoarthritisTenghuang Jiangu Tabletsnetwork pharmacologysignal pathwaymolecular docking

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