Identification and fingerprint of Qinsu Capsule based on high resolution mass spectrometer

Guangwei HUANG; Rendi SUN; Sude YANG; Yue WEI; Yunfei MA

doi:10.16305/j.1007-1334.2021.2005177

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Identification and fingerprint of Qinsu Capsule based on high resolution mass spectrometer

更新时间：2022-08-03

- Identification and fingerprint of Qinsu Capsule based on high resolution mass spectrometer
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 10, Pages: 58-65(2021)
- 作者机构：
  
  1.上海绿谷生命园医药有限公司（上海　201707）
  2.上海复方中药（绿谷）工程技术研究中心（上海　201707）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2005177
  CLC：
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Guangwei HUANG, Rendi SUN, Sude YANG, et al. Identification and fingerprint of Qinsu Capsule based on high resolution mass spectrometer. [J]. Shanghai Journal of Traditional Chinese Medicine 55(10):58-65(2021)
DOI：

Guangwei HUANG, Rendi SUN, Sude YANG, et al. Identification and fingerprint of Qinsu Capsule based on high resolution mass spectrometer. [J]. Shanghai Journal of Traditional Chinese Medicine 55(10):58-65(2021) DOI： 10.16305/j.1007-1334.2021.2005177.

摘要

目的,2,采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF/MS）和质谱数据库对芩苏胶囊（QSC）化学成分进行定性分析，并建立HPLC指纹图谱。,方法,2,采用Agilent ZORBAX RRHD SB-C,18,（2.1 mm×100 mm，1.8 μm）色谱柱，以乙腈-0.1%甲酸水溶液梯度洗脱，体积流量为0.3 mL/min，柱温为25 ℃，检测波长为270 nm。UPLC-Q-TOF/MS正、负2种离子模式扫描对QSC化学成分定性分析；采用Waters Xbridge Shield RP,18,（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-0.05%磷酸水溶液梯度洗脱，体积流量为0.8 mL/min，柱温为30 ℃，检测波长为270 nm，对10批QSC进行指纹图谱分析。,结果,2,从QSC中共检测到68个化学成分，鉴定出22个化学成分，指纹图谱确定了33个共有峰，10批QSC的指纹谱图相似度均大于0.990。,结论,2,定性分析了QSC的化学成分，建立了其指纹图谱，可为深入研究其物质基础和完善提升质量标准提供支持。

Abstract

Objective,2,To qualitatively analyze the chemical composition of Qinsu Capsule (QSC) by Ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) and mass spectrometry database, and establish HPLC fingerprint for QSC.,Methods,2,The Agilent ZORBAX RRHD SB-C,18,(2.1 mm×100 mm, 1.8 μm) was used with a mobile phase of acetonitrile-0.1% methanoic acid in gradient elution. The flow rate was 0.3 mL/min, the column temperature 25 ℃, and the detection wavelength 270 nm. UPLC-Q-TOF/MS was used for qualitative analysis in the positive and negative ion modes. The chromatographic fingerprints analysis of 10 batches of QSC were carried out in the Waters Xbridge Shield RP,18,(4.6 mm×250 mm, 5 μm) column with a mobile phase of acetonitrile-0.05% phosphoric acid in gradient elution, the flow rate 0.8 mL/min, the column temperature 30 ℃, and the detection wavelength 270 nm.,Results,2,Totally 68 chemical components were detected from QSC, and 22 of them were identified. The HPLC fingerprint for 10 batches of QSC was established and 33 common peaks were obtained. The similarity of 10 batches of QSC was over 0.990.,Conclusion,2,The chemical composition of QSC was qualitatively analyzed and HPLC fingerprint was established, which can provide support for the further study of substance basis and quality standard promotion of QSC.

关键词

芩苏胶囊超高效液相色谱-四极杆飞行时间质谱指纹图谱高分辨质谱数据库中药研究

Keywords

Qinsu CapsuleUPLC-Q-TOF/MSfingerprinthigh resolution mass spectrum database;traditional Chinese herbal medicine

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