Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats

Sisi NING; Shaozheng YANG; Jialin WANG; Jiahui JIN; Lei LUO; Lin LI; Yuxia SUN; Jiayi MA; Chunling ZHANG; Yi LI

doi:10.16305/j.1007-1334.2021.2004151

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Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats

更新时间：2022-08-03

- Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats
- Shanghai Journal of Traditional Chinese Medicine Vol. 55, Issue 4, Pages: 73-78(2021)
- 作者机构：
  
  1.上海中医药大学附属曙光医院（上海　200021）
  2.上海市奉贤区南桥镇社区卫生服务中心（上海　201408）
  3.上海市光华中西医结合医院（上海　200052）
  4.上海中医药大学附属第七人民医院（上海　200137）
  5.上海市黄浦区香山中医医院（上海　200020）；
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2021.2004151
  CLC：
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Sisi NING, Shaozheng YANG, Jialin WANG, et al. Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats. [J]. Shanghai Journal of Traditional Chinese Medicine 55(4):73-78(2021)
DOI：

Sisi NING, Shaozheng YANG, Jialin WANG, et al. Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats. [J]. Shanghai Journal of Traditional Chinese Medicine 55(4):73-78(2021) DOI： 10.16305/j.1007-1334.2021.2004151.

摘要

目的,2,观察针刺对自发性高血压模型大鼠（SHR）蛋白尿及病理的影响。,方法,2,将SHR大鼠随机分为模型组、氯沙坦组、肾俞组、膈俞组、肾俞+膈俞组，每组10只，另以10只WKY大鼠作为正常组。氯沙坦组予氯沙坦钾片溶于0.9%NaCl溶液，按30 mg·kg,-1,·d,-1,灌胃，每日1次；肾俞组、膈俞组、肾俞+膈俞组在相应腧穴予电针干预，隔日1次；模型组做与针刺组同等固定，不予针刺。各组均连续干预12周。分别于干预前、干预第4、8、12周末以生化法检测大鼠24 h尿蛋白定量，12周末取大鼠肾脏标本以光镜观察HE染色、电镜观察肾脏超微结构的病理变化，免疫组化技术检测肾组织Klotho蛋白的阳性表达面积。,结果,2,干预结束后，氯沙坦组及针刺各组24 h尿蛋白均低于模型组（,P,<,0.05）；氯沙坦组及针刺各组的肾脏病理损伤均有不同程度减轻；与模型组比较，氯沙坦组、针刺各组Klotho阳性面积表达均有所升高（,P,<,0.05）。,结论,2,针刺可通过调节肾脏Klotho的表达，进而减少SHR大鼠的蛋白尿、减轻肾脏病理损伤，延缓高血压肾损害进展。

Abstract

Objective,2,To observe the effect of acupuncture on proteinuria and renal pathology in spontaneous hypertension rats (SHR).,Methods,2,SHR rats were randomly divided into model group, losartan group, Shenshu acupuncture group, Geshu acupuncture group, and Shenshu + Geshu group, with 10 rats in each group, and 10 WKY rats were set as normal group. The losartan group was intragastrically administered with losartan tablets which were dissolved in normal saline, with 30 mg/kg once a day. The acupuncture groups were given with electro-acupuncture intervention according to the corresponding acupoints every other day. The model group was fixed like the acupuncture groups done and without acupuncture. All groups were intervened continuously for 12 weeks. 24-hour urinary protein was detected by biochemical method before intervention and at the end of 4th, 8th and 12th week of intervention. At the end of 12th week, kidney samples were taken to observe the pathological changes of renal ultrastructure by light microscope and electron microscope, and the positive expression area of Klotho protein in renal tissue was detected by immunohistochemistry.,Results,2,After intervention, the 24 h urinary protein in the losartan group and the acupuncture groups were lower than that in the model group(,P,<, 0.05). The renal pathological injury in the losartan group and the acupuncture groups were alleviated in varying degrees, and the expression of Klotho positive area in the losartan group and the acupuncture groups were higher than that in the model group (,P,<,0.05).,Conclusion,2,Acupuncture can regulate the expression of Klotho in kidney, reduce proteinuria and renal pathological damage in SHR rats, and delay the progression of hypertensive renal damage.

关键词

自发性高血压大鼠针刺Klotho蛋白尿肾脏病理

Keywords

spontaneous hypertensionratsacupunctureKlothoproteinuriarenal pathology

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