Objective,2,To explore the effect of Mingmu Jiedu Prescription (MJP) fumigation on herpes simplex keratitis(HSK)by means of heat and drug separation.,Methods,2,Ninety healthy New Zealand white rabbits were randomly divided into five groups: model group (,n,=18) , traditional Chinese medicine thermal fumigation group (,n,=18) , thermal fumigation group (,n,=18) , fumigant group (,n,=18) and normal group (,n,=18) .Except for the normal group, all the other groups were inoculated with HSV-1 virus to establish HSK mode by ocular surface scratching method. After modeling, the model group and normal group were not treated with drugs, while the traditional Chinese medicine thermal fumigation group was fumigated with MJP every day, the thermal fumigation group was fumigated with distilled water, and the fumigant group was treated with MJP fumigation liquid eye drops.The degree of keratopathy in each group was evaluated at 1 day before modeling and 1 day, 3 days and 7 days after modeling.On the 1st, 3rd and 7th days after treatment, 6 eyes in each group were randomly selected for detecting the expression of HSV-1 in corneal tissue by PCR method, the contents of IL-2 and IFN-γ in aqueous humor by ELISA method, and the protein expressions of CD4, CD8 in peripheral blood by Western blot method.,Results,2,On the 3rd and 7th days after treatment, the corneal HSV-1 contents in the traditional Chinese medicine thermal fumigation group, the fumigant group and the thermal fumigation group were lower than those in the model group (,P,<,0.05) .But there was no significant difference in the content of IFN-γ in aqueous humor among the three groups (,P,>,0.05) .On the 7th day after treatment, there was interaction between traditional Chinese medicine thermal fumigation group and thermal fumigation group. On the 1st, 3rd and 7th days after treatment, the content of IL-2 in aqueous humor of the traditional Chinese medicine thermal fumigation group was significantly different from that of the model group, and there was significant difference between the fumigant group and the model group on the 1st and 7th days after treatment (,P,<,0.05) .The expression of CD4 protein in peripheral blood in the traditional Chinese medicine thermal fumigation group was significantly higher than that in the model group after 3 and 7 days of treatment (,P,<,0.05) .,Conclusion,2,MJP fumigation treatment can eliminate HSV-1 virus in HSK corneal tissue through thermal and drug effects, affect the level of local immune factor IL-2, and up-regulate the expression of CD4 in peripheral blood.