Transcriptome study on hippocampus of rats with kidney-not-storing-will insomnia

REN Xiaojuan; WANG Qingquan; ZHANG Xingping; WANG Guanying

doi:10.16305/j.1007-1334.2020.2006054

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Transcriptome study on hippocampus of rats with kidney-not-storing-will insomnia

更新时间：2022-08-31

- Transcriptome study on hippocampus of rats with kidney-not-storing-will insomnia
- Shanghai Journal of Traditional Chinese Medicine Vol. 54, Issue 12, Pages: 71-78(2020)
- 作者机构：
  
  1. 新疆医科大学附属中医医院内科,新疆,乌鲁木齐,830000
  2. 新疆维吾尔自治区乌鲁木齐市中医医院内科,新疆,乌鲁木齐,830002
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2020.2006054
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REN Xiaojuan, WANG Qingquan, ZHANG Xingping, et al. Transcriptome study on hippocampus of rats with kidney-not-storing-will insomnia. [J]. Shanghai Journal of Traditional Chinese Medicine 54(12):71-78(2020)
DOI：

REN Xiaojuan, WANG Qingquan, ZHANG Xingping, et al. Transcriptome study on hippocampus of rats with kidney-not-storing-will insomnia. [J]. Shanghai Journal of Traditional Chinese Medicine 54(12):71-78(2020) DOI： 10.16305/j.1007-1334.2020.2006054.

摘要

目的：探究肾不藏志不寐模型大鼠与正常大鼠海马差异基因(DEGs)的表达。方法：20只SD大鼠随机分为空白组和肾不藏志不寐组,每组10只。肾不藏志不寐组采用D-半乳糖(D-gal)皮下注射联合对氯苯丙氨酸(PCPA)腹腔注射的方法建立疾病模型,空白组予以等体积的0.9%NaCl溶液注射。模型建立后对两组大鼠海马进行转录组测序,筛选两组DEGs,并对DEGs 进行GO功能富集与KEGG通路分析,用实时荧光定量PCR(RT-qPCR)对显著DEGs的表达量进行验证。结果：与空白组比较,肾不藏志不寐组大鼠海马的差异基因有1 628个,其中上调 887个,下调 741 个；差异基因的功能涉及神经发育、细胞发育、三酰甘油代谢、核糖体、氧化应激、炎性因子；并与帕金森病、阿尔茨海默病、逆行内源性大麻素信号传导通路、慢性进行性舞蹈病有密切联系。与空白组比较,肾不藏志不寐组大鼠海马脂肪酸酰胺水解酶(Faah)mRNA表达明显下调(P<0.05),DNA拓扑异构酶Ⅱ(Top2a)mRNA的表达下调,但差异无统计学意义(P＞0.05)；鸟嘌呤核肽结合蛋白编码基因β3亚基(Gnb3)和基质金属蛋白酶-9(MMP-9)mRNA表达均明显上调(P<0.05)。结论：肾不藏志不寐与神经、代谢和炎症通路有关；Faah、Gnb3、MMP-9为肾不藏志不寐模型大鼠海马的差异基因。

Abstract

Objective:To explore the expression of differentially expressed genes (DEGs) in the hippocampus of insomnia rat model with kidney-not-storing-will type. MethodsTwenty SD rats were randomly divided into two groups:the blank group and the kidney-not-storing-will insomnia group (model group),with 10 rats in each group. The disease model was induced by subcutaneous injection of D-galactose (D-gal) combined with intraperitoneal injection of para-chlorophenylalanine (PCPA)in the kidney-not-storing- will insomnia group and the rats in the blank group were given the same volume of 0.9% NaCl solution. The transcriptome sequencing was performed on the hippocampus of the rats in the two groups,and DEGs were screened after the establishment of the rat model. Moreover,GO enrichment and KEGG pathway analysis of DEGs were carried out,and the expression of significant DEGs was verified by real-time fluorescence quantitative PCR (RT-qPCR). Results:Compared with the blank group,there were 1 628 DEGs in the model group,of which 887 DEGs were up-regulated and 741 DEGs were down-regulated. The DEGs were mainly involved in neuro development,cell development,triglyceride metabolism,ribosomes,oxidative stress and inflammatory factors. The DEGs were closely associated with Parkinson’s disease,Alzheimer’s disease,retrograde endocannabinoid signaling transduction pathway and Huntington’s disease. Compared with the blank group,the expression of fatty acid amide hydrolase (Faah) mRNA on hippocampus was significantly down-regulated (P<0.05),and the expression of DNA topoisomerase II (Top2a) mRNA was down-regulated,but there was no statistically significant difference (P＞0.05). The expression of guanine nucleotide binding protein coding gene 3 subunit (Gnb3) and matrix metalloproteinase (MMP-9) were both significantly up-regulated (P<0.05). Conclusion:The kidney-not-storing-will insomnia was related to nerve,metabolism and inflammation pathways. Faah,Gnb3 and MMP-9 were the DEGs in the hippocampus of rats with kidney-not-storing-will insomnia.

关键词

肾不藏志不寐模型转录组学大鼠

Keywords

failure ofkidney storing willinsomnia modeltranscriptomicsrats

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