Intervention of Guanxinkang in cholesterol metabolism of HepG2 cells through miRNA

CHEN Ran; JIN Qipeng; MA Guiping; ZHOU Zhongyan; ZHAO Wairong; LIU Ping; ZHANG Yiyi

doi:10.16305/j.1007-1334.2020.2006012

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Intervention of Guanxinkang in cholesterol metabolism of HepG2 cells through miRNA

更新时间：2022-08-31

- Intervention of Guanxinkang in cholesterol metabolism of HepG2 cells through miRNA
- Shanghai Journal of Traditional Chinese Medicine Vol. 54, Issue 11, Pages: 71-77(2020)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院重症监护室,上海,200032
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2020.2006012
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CHEN Ran, JIN Qipeng, MA Guiping, et al. Intervention of Guanxinkang in cholesterol metabolism of HepG2 cells through miRNA. [J]. Shanghai Journal of Traditional Chinese Medicine 54(11):71-77(2020)
DOI：

CHEN Ran, JIN Qipeng, MA Guiping, et al. Intervention of Guanxinkang in cholesterol metabolism of HepG2 cells through miRNA. [J]. Shanghai Journal of Traditional Chinese Medicine 54(11):71-77(2020) DOI： 10.16305/j.1007-1334.2020.2006012.

摘要

目的：研究冠心康对不同浓度氧化低密度脂蛋白(ox-LDL)诱导HepG2细胞后胆固醇水平及胆固醇代谢相关miR-33a、miR-122以及低密度脂蛋白受体(LDLR)的影响。方法：制备冠心康和辛伐他汀含药血清,将HepG2细胞随机分为空白对照组、模型组、辛伐他汀组和冠心康组,分别用20、40、80、160 mg/L浓度的ox-LDL诱导HepG2细胞24 h后,再用各组含药血清孵育48 h。采用油红O染色法观察HepG2胆固醇代谢模型的细胞形态,实时荧光定量PCR法检测miR-33a、miR-122、LDLR基因表达水平,Western blot法检测LDLR蛋白表达。结果：与模型组比较,冠心康可显著减少不同浓度ox-LDL诱导后HepG2细胞中橙色脂滴含量,上调ox-LDL诱导后细胞中LDLR基因和蛋白的表达,并抑制miR-33a和miR-122的表达(P<0.05,P<0.01)。结论：冠心康可通过上调LDLR基因和蛋白的表达,抑制miR-33a和miR-122的表达,从而降低细胞中胆固醇含量,缓解胆固醇代谢失衡进程。

Abstract

Objective:To research the effect of Guanxinkang on the cholesterol level of HepG2 cells induced by ox-LDL at different concentrations,as well as the cholesterol metabolism-related miR-33a,miR-122 and LDLR. MethodsSerum containing Guanxinkang and simvastatin were prepared respectively,and HepG2 cells were randomly divided into blank control group,model group,simvastatin group and Guanxinkang group. HepG2 cells were induced by ox-LDL with concentrations of 20,40,80 and 160 mg/L respectively for 24 hours,and then incubated with serum containing drugs for 48 hours respectively. The cell morphology of HepG2 cholesterol metabolism model was observed by oil red O staining. The expression levels of mir-33a,mir-122 and LDLR genes were detected by real-time PCR,and the protein expression of LDLR was detected by Western blot. Results:Compared with the model group, Guanxinkang can significantly reduce the content of orange lipid droplets in HepG2 cells after ox-LDL induction at different concentrations,up-regulate the expression of LDLR gene and protein in the cells after ox-LDL induction,and inhibit the expression of miR-33a and miR-122 gene(P<0.05, P<0.01). Conclusion:Guanxinkang can up-regulate the expression of LDLR gene and protein,inhibit the expression of miR-33a and miR-122 gene,thereby reducing the content of cholesterol in cells and alleviating the process of cholesterol metabolism imbalance.

关键词

冠心康胆固醇代谢LDLRmiR-33amiR-122HepG2细胞大鼠

Keywords

Guanxinkangcholesterol metabolismLDLRmiR-33 amiR-122HepG2 cellrats

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