Analysis of intestinal microbiota characteristics in ulcerative colitis patients with thin white fur and yellow greasy fur

LI Dan; YAO Yingying; DAI Yancheng; ZHANG Yali; TANG Yingjue; ZHU Ruya; SHI Yu; TANG Zhipeng

doi:10.16305/j.1007-1334.2020.2003314

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Analysis of intestinal microbiota characteristics in ulcerative colitis patients with thin white fur and yellow greasy fur

更新时间：2022-08-31

- Analysis of intestinal microbiota characteristics in ulcerative colitis patients with thin white fur and yellow greasy fur
- Shanghai Journal of Traditional Chinese Medicine Vol. 54, Issue 12, Pages: 15-20(2020)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院,上海中医药大学脾胃病研究所,上海,200032
  2. 上海交通大学医学院附属同仁医院中医科,上海,200336
  3. 上海中医药大学附属上海市中西医结合医院脾胃病科,上海,200082
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2020.2003314
  CLC：
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LI Dan, YAO Yingying, DAI Yancheng, et al. Analysis of intestinal microbiota characteristics in ulcerative colitis patients with thin white fur and yellow greasy fur. [J]. Shanghai Journal of Traditional Chinese Medicine 54(12):15-20(2020)
DOI：

LI Dan, YAO Yingying, DAI Yancheng, et al. Analysis of intestinal microbiota characteristics in ulcerative colitis patients with thin white fur and yellow greasy fur. [J]. Shanghai Journal of Traditional Chinese Medicine 54(12):15-20(2020) DOI： 10.16305/j.1007-1334.2020.2003314.

摘要

目的：通过分析溃疡性结肠炎(UC)薄白苔、黄腻苔患者的肠道菌群特征,探讨UC患者舌苔与肠道菌群之间的关系。方法：选取UC薄白苔患者、UC黄腻苔患者、健康对照者各35例,分别设为UC薄白苔组、UC黄腻苔组、健康对照组。收集粪便标本,采用16S rDNA基因测序分析技术和生物信息学方法分析比较各组肠道菌群的多样性和丰度。结果：①与健康对照组比较,UC薄白苔、黄腻苔组患者肠道菌群物种的丰富度和多样性降低(P＜0.001),肠道菌群物种数量减少；与UC薄白苔组比较,UC黄腻苔组患者肠道菌群丰富度差异无统计学意义(P＞0.05),但肠道菌群物种数量减少。②门水平差异分析:与健康对照组比较,UC薄白苔、黄腻苔组患者肠道菌群中厚壁菌门(Firmicutes)减少(P＜0.05)；与UC薄白苔组比较,UC黄腻苔组患者肠道菌群中蓝细菌门(Cyanobacteria)增多(P＜0.05)。③属水平差异分析:与健康对照组比较,UC薄白苔组患者肠道菌群中埃希氏-志贺氏菌属(Escherichia-Shigella)、链球菌属(Streptococcus)等增多(P＜0.05),罕见小球菌属(Subdoligranulum)、多尔氏菌属(Dorea)、瘤胃球菌属(Ruminococcus)、罗氏菌属(Roseburia)等减少(P＜0.05)；UC黄腻苔组患者肠道菌群中埃希氏-志贺氏菌属(Escherichia-Shigella)、乳杆菌属(Lactobacillus)等增多(P＜0.05),多尔氏菌属(Dorea)、罕见小球菌属(Subdoligranulum)、瘤胃球菌属(Ruminococcus)、考拉杆菌属(Phascolarctobacterium)、罗氏菌属(Roseburia)等减少(P＜0.05)。与UC薄白苔组比较,UC黄腻苔组患者肠道菌群链球菌(Streptococcus)增多(P＜0.05),巨球型菌属(Megasphaera)、拟普氏菌属(Alloprevotella)、普氏菌属(Prevotella)、奈瑟氏菌属(Neisseria)等减少(P＜0.05)。④LDA判定(纲、目、科水平):UC薄白苔组患者肠道菌群中普氏菌科(f_Prevotellaceae)、奈瑟氏球菌目(o_Neisseriales)、奈瑟氏球菌科(f_Neisseriaceae)丰度显著多于UC黄腻苔组(LDA＞2.0),UC黄腻苔组患者肠道菌群中杆菌纲(c_Bacilli)、乳杆菌目(o_Lactobacillales)、链球菌科(f_Streptococcaceae)、消化链球菌科(f_Peptostreptococcaceae)丰度显著高于UC薄白苔组(LDA＞2.0)。结论：UC薄白苔、黄腻苔患者肠道菌群丰富度和多样性均较健康者明显下降；UC薄白苔患者和UC黄腻苔患者在肠道菌群组成和细菌丰度上均存在差异,黄腻苔患者肠道致病性细菌增多而具有潜在保护作用的细菌减少；肠道菌群检测可以为舌诊现代化提供一定的信息。

Abstract

Objective:To analyze the intestinal microbiota characteristics in ulcerative colitis (UC) patients with thin white fur (TWF) and yellow greasy fur (YGF), and to explore the relationship between tongue fur and intestinal microbiota in UC patients. MethodsThirty-five UC patients with TWF, 35 UC patients with YGF and 35 healthy persons were included to form UCTWF group, UCYGF group and healthy control (HC) group respectively. Fecal specimens were collected, and 16S rDNA gene sequencing and bioinformatics methods were used to analyze and compare the diversity and abundance of intestinal microbiota in each group. Results:①Compared with HC group, the abundance and diversity of intestinal microbiota decreased significantly (P<0.001) in UCTWF group and UCYGF group, and the number of intestinal microbiota species decreased. Compared with UCTWF group, there was no significant difference in intestinal microbiota abundance in UCYGF group (P>0.05), but the number of intestinal microbiota species decreased. ②At the phylum level: compared with HC group, the intestinal microbiota of UCTWF group and UCYGF group decreased in the content of Firmicutes (P<0.05). Compared with UCTWF group, the intestinal microbiota of UCYGF group increased in the content of Cyanobacteria (P<0.05). ③At the genus level: Compared with HC group, the contents of Escherichia-Shigella and Streptococcus increased (P<0.05), while the contents of Subdoligranulum, Dorea, Ruminococcus and Roseburia decreased (P<0.05) in the intestinal microbiota of UCTWF group; Compared with HC group, the contents of Escherichia-Shigella and Lactobacillus increased (P<0.05), while the contents of Dorea, Subdoligranulum, Ruminococcus, Phascolarctobacterium and Roseburia decreased (P<0.05) in the intestinal microbiota of UCYGF group. Compared with UCTWF group, the content of Streptococcus increased (P<0.05) while the contents of Megasphaera, Alloprevotella, Prevotella and Neisseria decreased (P<0.05) in intestinal microbiota of UCYGF group. ④At the levels of class, order and family, the abundance of f_Prevotellaceae, o_Neisseriales and f_Neisseriaceae in the intestinal microbiota of UCTWF group was significantly higher than that of UCYGF group (LDA>2.0). The abundance of c_Bacilli, o_Lactobacillales, f_Streptococccaceae and f_Peptostreptococccaceae in UCYGF group was significantly higher than that in UCTWF group (LDA>2.0). Conclusion:sThe abundance and diversity of intestinal microbiota in UC patients with thin white fur and yellow greasy fur were significantly lower than those in healthy patients. There are differences in intestinal microbiota composition and abundance between UC patients with thin white fur and UC patients with yellow greasy fur. Pathogenic bacteria increase while bacteria with potential protective effect decrease in the intestinal tract of UC patients with yellow greasy fur. Intestinal microbiota detection can provide valuable information for the modernization of tongue diagnosis.

关键词

溃疡性结肠炎舌苔肠道菌群舌诊16S rDNA基因测序技术

Keywords

ulcerative colitistongue furintestinal microbiotatangue diagnosis16S rDNA gene sequencing

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