Study on molecular mechanism of Qidongning Formula(QDF)suppressing lung cancer cells proliferation by regulating autophagy

WANG Chengyan; JIAO Lijing; ZHAO Bei; YANG Wenxiao; BI Ling; XU Ling

doi:10.16305/j.1007-1334.2020.2002148

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Study on molecular mechanism of Qidongning Formula(QDF)suppressing lung cancer cells proliferation by regulating autophagy

更新时间：2022-08-31

- Study on molecular mechanism of Qidongning Formula(QDF)suppressing lung cancer cells proliferation by regulating autophagy
- Shanghai Journal of Traditional Chinese Medicine Vol. 54, Issue 11, Pages: 60-63(2020)
- 作者机构：
  
  1. 上海中医药大学附属岳阳中西医结合医院肿瘤科,上海,200437
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2020.2002148
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WANG Chengyan, JIAO Lijing, ZHAO Bei, et al. Study on molecular mechanism of Qidongning Formula(QDF)suppressing lung cancer cells proliferation by regulating autophagy. [J]. Shanghai Journal of Traditional Chinese Medicine 54(11):60-63(2020)
DOI：

WANG Chengyan, JIAO Lijing, ZHAO Bei, et al. Study on molecular mechanism of Qidongning Formula(QDF)suppressing lung cancer cells proliferation by regulating autophagy. [J]. Shanghai Journal of Traditional Chinese Medicine 54(11):60-63(2020) DOI： 10.16305/j.1007-1334.2020.2002148.

摘要

目的：研究芪冬宁方对自噬的影响及其对肺癌细胞生长的作用。方法：①采用CCK8法检测不同浓度的芪冬宁方对A549、NCI-H460存活率的影响；克隆形成实验检测不同浓度的芪冬宁方对A549、NCI-H460克隆形成能力的影响；Western blot法检测不同浓度及时间点,芪冬宁方对A549、NCI-H460细胞自噬相关蛋白LC3B、p62表达的影响；免疫荧光检测芪冬宁方对LC3B表达的影响；采用CCK8法及克隆形成实验检测芪冬宁方对自噬抑制状态下A549、NCI-H460细胞存活率及克隆形成能力的影响。②以裸鼠 NCI-H460皮下移植瘤为模型,观察芪冬宁方对瘤体的抑制作用,并应用Western blot法检测各组瘤体自噬相关蛋白LC3B、p62表达的差异。结果：体外实验表明,芪冬宁方可以抑制A549、NCI-H460细胞增殖。自噬抑制状态下,芪冬宁方的抗A549、NCI-H460细胞增殖能力下降。Western blot结果显示,芪冬宁方可上调A549、NCI-H460细胞LC3B水平的表达,下调p62的表达。免疫荧光结果显示,芪冬宁方可以使弥散的LC3B聚集,呈斑点状。动物实验显示,芪冬宁方可以抑制裸小鼠NCI-H460皮下移植瘤瘤体的生长,可以上调LC3B的表达,下调p62的表达。结论：芪冬宁方可通过调控自噬抑制肺癌的生长。

Abstract

Objective:To explore the effects of Qidongning Formula(QDF) on lung cancer cells proliferation by regulating autophagy. Methods①The effects of different concentrations of QDF on the survival rate of A549 and NCI-H460 were detected by CCK8 assay, and the effects on the clone formation ability of A549 and NCI-H460 by clone formation assay. The effects of QDF on the expression of autophagy-associated proteins LC3B and p62 in A549 and NCI-H460 cells were detected by Western blot method, and the effect of QDF on the expression of LC3B was detected by immunofluorescence. The survival rates and colony formation ability of A549 and NCI-H460 cells under the inhibition of autophagy were detected by CCK8 assay and clone formation assay.②The subcutaneous transplanted tumor of NCI-H460 in nude mice was used as a model to observe the inhibitory effect of QDF on the tumor, and the expression of autophagy-related proteins LC3B and p62 in each group was detected by Western blot method. Results: The experiment in vitro showed that QDF could inhibit the proliferation of A549 and NCI-H460 cells. Under the condition of autophagy inhibition, the ability decreased of QDF against A549 and NCI-H460 cells proliferation. Western blot results showed that QDF could up-regulate the expression of LC3B and down-regulate the expression of p62 in A549 and NCI-H460 cells. The immunofluorescence results showed that QDF could concentrate the dispersed LC3B in the form of speckled. Animal experiments showed that QDF could inhibit the growth of NCI-H460 subcutaneously transplanted tumor in nude mice, up-regulate the expression of LC3B and down-regulate the expression of p62. Conclusion:sQDF could suppress lung cancer cells proliferation by regulating autophagy.

关键词

芪冬宁方肺癌自噬抑制增殖肿瘤细胞

Keywords

QDFlung cancerautophagysuppressproliferationcancer cell

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