Effect and mechanism of lycium barbarum polysaccharide in the treatment of hypoandrogenic male sterile rats

QU Lijuan; SONG Yanqiu; ZHANG Yan; ZHONG Ruihua; YANG Wenjie; LI Guoting; XIE Shuwu

doi:10.16305/j.1007-1334.2020.1911117

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Effect and mechanism of lycium barbarum polysaccharide in the treatment of hypoandrogenic male sterile rats

更新时间：2022-08-31

- Effect and mechanism of lycium barbarum polysaccharide in the treatment of hypoandrogenic male sterile rats
- Shanghai Journal of Traditional Chinese Medicine Vol. 54, Issue 9, Pages: 88-92(2020)
- 作者机构：
  
  1. 江苏大学医学院附属上海市第八人民医院检验科,上海,200235
  2. 江苏大学医学院附属上海市第八人民医院心内科,上海,200235
  3. 江苏大学医学院附属上海市第八人民医院科教科,上海,200235
  4. 复旦大学生殖与发育研究院上海市计划生育科学研究所生殖药理组,国家卫生健康委员会计划生育药具重点实验室,上海,200032
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2020.1911117
  CLC：
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QU Lijuan, SONG Yanqiu, ZHANG Yan, et al. Effect and mechanism of lycium barbarum polysaccharide in the treatment of hypoandrogenic male sterile rats. [J]. Shanghai Journal of Traditional Chinese Medicine 54(9):88-92(2020)
DOI：

QU Lijuan, SONG Yanqiu, ZHANG Yan, et al. Effect and mechanism of lycium barbarum polysaccharide in the treatment of hypoandrogenic male sterile rats. [J]. Shanghai Journal of Traditional Chinese Medicine 54(9):88-92(2020) DOI： 10.16305/j.1007-1334.2020.1911117.

摘要

目的：通过观察枸杞多糖对低雄激素不育大鼠生殖损伤的改善作用,评价枸杞多糖治疗低雄激素雄性不育的药效和可能的作用机制。方法：将雄性成年SD大鼠随机分为空白对照组、模型对照组、枸杞多糖低剂量和高剂量组,其中造模给药方式为灌胃给予度他雄胺40 mg/kg,1次/d,连续28 d；在造模的第15天开始枸杞多糖干预灌胃给药,高低剂量组分别给予枸杞多糖80 mg/kg和40 mg/kg,模型对照组给予等量溶媒,1次/d,连续14 d。给药结束时检测睾丸附睾组织学、超微结构、精子活力、交配雌鼠受孕率、血清雄激素水平、附睾雄激素受体(AR)和核转录因子(NF-κB)蛋白表达。结果：与模型对照组比较,枸杞多糖高剂量组精子活率和前向运动率显著升高(P＜0.05)；枸杞多糖低剂量组和高剂量组交配雌鼠受孕率分别为37.5%和62.5%,较模型对照组分别上升25%和50%；模型对照组附睾可见附睾上皮连接出现断裂,管内精子聚集；枸杞多糖低剂量组大鼠附睾上皮结构和精子排列有一定的恢复,高剂量组附睾上皮组织和精子基本恢复正常；枸杞多糖给药组血清双氢睾酮(DHT)水平较模型对照组显著升高(P＜0.05,P＜0.01)；枸杞多糖给药组AR和NF-κB蛋白表达较模型对照组显著升高(P＜0.05,P＜0.01)。结论：枸杞多糖可以改善低雄激素附睾不育大鼠的生育能力,其作用机制可能与升高血清DHT水平、促进AR和NF-κB表达、发挥雄激素样活性有关。

Abstract

Objective:To observe the effect of lycium barbarum polysaccharide (LBP)on the reproductive damage of hypoandrogen male sterile rats,and to evaluate the efficacy and possible mechanism of LBP in the treatment of hypoandrogen male sterility. MethodsMale adult SD rats were randomly divided into blank control group,model control group,low-dose and high-dose LBP group. The model was made by intragastric administration of dutasteride 40 mg / kg once a day for 28 days,and LBP was given by gavage at the 15th day of modeling. LBP 80 mg / kg and 40 mg / kg were given in the high-dose and the low-dose LBP groups respectively and the model control group was given the same amount of vehicle for 14 days. At the end of administration,testicular and epididymal histology, ultrastructure, sperm activity,pregnancy rate,serum androgen level,epididymal androgen receptor (AR)and nuclear factor kappa-B (NF-κB)protein were detected. Results:compared with the model control group,the sperm viability and forward movement rate in the high-dose LBP group were significantly higher (P＜0.05). The pregnancy rate was 37.5% and 62.5% in the low-dose and the high-dose LBP group,respectively,which was 25% and 50% higher than that in the model control group. The epididymal epithelial junction was broken and sperms gathered in the epididymis in the model control group. The epididymal epithelial structure and sperm arrangement were recovered to a certain extent in the low-dose LBP group and the epididymal epithelium and sperms in the high-dose LBP group basically recovered to normal. The serum dihydrotestosterone(DHT) in the both LBP groups was significantly higher than that in model control group (P＜0.05,P＜0.01); the expression of AR and NF-κB protein in the both LBP groups was significantly higher than that in model control group(P＜0.05,P＜0.01). Conclusion:LBP can improve the fertility of hypoandrogenic epididymal infertility rats,which may be related to the increase of serum DHT level,the promotion of AR and NF-κB expression and the exertion of androgen like activity.

关键词

不育低雄激素大鼠枸杞多糖双氢睾酮附睾雄激素受体核转录因子

Keywords

infertilityhypoandrogenratslycium barbarum polysaccharidedihydrotestosterone(DHT)epididymal androgen receptornuclear factor kappa-B(NF-κB)

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