Mechanism of Xiayuxue Decoction inhibiting acute liver injury induced by lipopolysaccharide through regulating neutrophil infiltration

YANG Guangyue; TAO Le; SHEN Dongxiao; MA Wenting; ZHANG Wei; WU Liu; LIU Cheng

doi:10.16305/j.1007-1334.2020.1910136

您当前的位置：

首页 >

文章列表页 >

Mechanism of Xiayuxue Decoction inhibiting acute liver injury induced by lipopolysaccharide through regulating neutrophil infiltration

更新时间：2022-08-31

- Mechanism of Xiayuxue Decoction inhibiting acute liver injury induced by lipopolysaccharide through regulating neutrophil infiltration
- Shanghai Journal of Traditional Chinese Medicine Vol. 54, Issue 10, Pages: 87-92(2020)
- 作者机构：
  
  1. 上海中医药大学附属普陀医院中心实验室,上海,200062
  2. 上海中医药大学附属普陀医院感染科肝病实验室,上海,200062
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2020.1910136
  CLC：
扫描看全文
YANG Guangyue, TAO Le, SHEN Dongxiao, et al. Mechanism of Xiayuxue Decoction inhibiting acute liver injury induced by lipopolysaccharide through regulating neutrophil infiltration. [J]. Shanghai Journal of Traditional Chinese Medicine 54(10):87-92(2020)
DOI：

YANG Guangyue, TAO Le, SHEN Dongxiao, et al. Mechanism of Xiayuxue Decoction inhibiting acute liver injury induced by lipopolysaccharide through regulating neutrophil infiltration. [J]. Shanghai Journal of Traditional Chinese Medicine 54(10):87-92(2020) DOI： 10.16305/j.1007-1334.2020.1910136.

摘要

目的：以脂多糖(LPS)诱导的急性肝损伤为载体,探讨下瘀血汤对急性肝损伤的干预作用。方法：将24只雄性C57BL/6小鼠随机分为正常组、模型组、下瘀血汤组,每组8只；下瘀血汤组按照0.467 8 g/kg剂量一次性灌胃下瘀血汤药液(相当于75 kg成人体质量的10倍量),正常组和模型组给予等量的双蒸水,4 h后下瘀血汤组和模型组小鼠腹腔注射LPS(10 mg/kg),18 h后取材检测肝功能、病理学,免疫组化检测中性粒细胞标记物髓过氧化物酶(MPO)及CXC趋化因子配体15(CXCL15)表达,实时定量PCR检测肿瘤坏死因子(TNF-α)、单核细胞趋化蛋白-1(MCP-1)、CXC趋化因子配体2(CXCL2)、CC趋化因子配体3(CCL3)、CXC趋化因子受体1(CXCR1)、白介素6(IL-6)基因表达。结果：与正常组比较,模型组肝功能ALT和AST水平显著升高(P<0.001),下瘀血汤对LPS诱导的肝功能损伤有显著改善作用(P<0.05)；组织病理学显示模型组肝组织炎细胞浸润明显,免疫组化染色显示LPS处理后MPO及CXCL15阳性表达显著升高(P<0.001),主要分布在小叶间和肝窦,下瘀血汤显著抑制中性细胞浸润。实时定量PCR结果显示,模型组TNF-α、IL-6炎症因子及趋化因子MCP-1、CXCL2、CCL3、CXCR1的mRNA表达显著升高(P<0.001)；下瘀血汤对LPS引起的炎症/趋化因子水平升高有显著抑制作用(P<0.01)。结论：LPS诱导肝脏中性粒细胞浸润,上调炎症及趋化因子表达；下瘀血汤对LPS诱导的肝损伤有显著的抑制作用。

Abstract

Objective:To investigate the intervention effect of Xiayuxue Decoction(XYXD) on acute liver injury induced by lipopolysaccharide (LPS). MethodsTwenty-four male C57BL/6 mice were randomly divided into normal group(n=8),model group(n=8) and XYXD group (n=8). The mice in the XYXD group were given intragastric administration of XYXD at a dose of 0.4 678 g/kg (equivalent to 10 times that of the adult body mass of 75 kg), and the mice in the normal group and the model group were given the same amount of double distilled water. Four hours later, the mice in the XYXD group and the model group were injected intraperitoneally with LPS (10 mg/kg). After 18 hours, the liver function and histopathology were detected. The expressions of myeloperoxidase (MPO) and CXC chemokine ligand 15 (CXCL15) were detected by immunohistochemistry, and the gene expressions of tumor necrosis factor (TNF- α), monocyte chemotactic protein-1 (MCP-1), CXC chemokine ligand 2 (CXCL2), CC chemokine ligand 3 (CCL3), CXC chemokine receptor 1 (CXCR1) and interleukin 6 (IL-6) were detected by real-time PCR. Results:Compared with the normal group, the levels of ALT and AST in the model group were significantly increased (P<0.001), and XYXD could significantly improve the liver function injury induced by LPS (P<0.05). Histopathology showed that the infiltration of inflammatory cells in liver tissue was obvious in the model group, and the positive expressions of MPO and CXCL15 were significantly increased after LPS treatment (P<0.001), mainly distributed in interlobule and hepatic sinusoid, and XYXD significantly inhibited neutrophil infiltration. The results of real-time quantitative PCR showed that the mRNA expressions of TNF- α, IL-6 inflammatory cytokines and chemokines including MCP-1, CXCL2, CCL3 and CXCR1 in the model group increased significantly (P<0.001), and XYXS could significantly inhibit the increase of inflammatory cytokines / chemokines induced by LPS (P<0.01). Conclusion:LPS induces neutrophil infiltration and up-regulates the expressions of inflammation cytokines and chemokines. XYXD has a significant inhibitory effect on liver injury induced by LPS.

关键词

急性肝损伤脂多糖小鼠中性粒细胞下瘀血汤

Keywords

acute liver injuryLPSmiceneutrophilXYXDXiayuxue Decoction

references

Views

263

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Common molecular mechanism of aristolochic acid I inducing hepatorenal toxicity

Xiaoxuming Decoction regulates NLRP3 ubiquitination level by down⁃regulating USP9X in treatment of acute lung injury

Effects of Qinfei Oral Liquid on airway inflammation and gut microbiota in asthmatic mice

Optimization of active components compatibility of Jiangzhi Granule to improve NAFLD based on weighed modification method

Effect of Bushen Huoxue Formula on expression of VEGF/PI3K/Akt pathway in decidua of mice with recurrent abortion

Related Author

No data

Related Institution

Second Department of Hepatology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Institute of Liver Diseases， Shanghai University of Traditional Chinese Medicine

Shanghai Key Laboratory of Traditional Chinese Clinical Medicine

MOE Key Laboratory of Liver and Kidney Diseases， Shanghai University of Traditional Chinese Medicine

Institutes of Integrative Medicine， Zhongshan Hospital， Fudan University

⁰