Objective:To study the skincare effect of collagen peptide from Sika antlerbase and to optimize the process of extracting the collagen peptide by enzymatic hydrolysis. MethodsThe collagen extracted by hot water method as the raw material was digested with trypsin. The degree of collagen hydrolysis was used as an index. The single-factor test and Box-Behnken design test were used to optimize the preparation process of collagen peptide from the antlerbase. The antioxidation and reducing ability of the collagen peptide were investigated in the concentration range of 10,20,30,40 and 50 g/mL. Forty Kunming mice were randomly divided into blank group,control group,low,middle and high dose collagen peptide solution groups (0.1 g/L,0.2 g/L,0.4 g/L).The skin of mice was smeared with the peptide twice a day. At the end of 6 weeks,the activity of SOD,the content of hydroxyproline (Hyp) and the level of MDA were measured.  Results:The antlerbase collagen comtent is 22.46%. The optimal preparation process of the antlerbase collagen peptide was enzymatic hydrolysis time 2.2 h,pH 8.0,temperature 50 ℃,enzyme amount 1 200 μ/g. Under this condition,the degree of protein hydrolysis is 15.97%.The collagen peptide of the antlerbase had the strongest antioxidation and reducing ability when the concentration reached 50 mg/mL. Compared with the blank group,the low,middle and high dose groups of collagen peptide had significant skincare effect as SOD and Hyp significantly increased (P<0.01) and MDA significantly decreased (P<0.01).  Conclusion:The optimized enzymatic extraction conditions are stable and feasible,and the antlerbase collagen peptide has a good skincare effect.