Objective:To observe the effect of electroacupuncture at Zusanli and Changqiang points on the physiological function of anal sphincter in rats with neurogenic fecal incontinence and to explore its mechanism. MethodsNinety healthy male SD rats were selected and randomly divided into the model group, electrical stimulation group and electroacupuncture group, with 30 rats in each group, and another 6 healthy male SD rats were set as blank group. Except the blank group, the fecal incontinence models were established in other three groups of rats by cutting off bilateral pudendal nerves. After successful modeling, the electroacupuncture group and the electrical stimulation group were continuously intervened for 14 days, while the model group and the blank group were not intervened. The stool condition of rats was observed, and related indexes of the physiological function of anal sphincter (anorectal manometry and myoelectric activity of external anal sphincter) were detected. HE staining was used to observe the pathological changes of anal sphincter tissue of rats in each group.  Results:①=1\*GB3In the blank group, the padding in the box was dry and the feces were granular. In the model group, the padding was wet and the feces were thin and mushy. The feces of rats in the electrical stimulation group and the electroacupuncture group gradually formed from mush to soft spherical shape. The feces in the electroacupuncture group improved more significantly and their texture was harder. ②=2\*GB3Compared with the blank group, the anorectal manometry in the model group decreased (P<0.05), and the myoelectric activity of the external anal sphincter decreased (P<0.05). ③=3\*GB3Compared with the model group, there was no significant difference in anorectal manometry and myoelectric activity of external anal sphincter in the electrical stimulation group at each observation time point (P>0.05). In the electroacupuncture group, the anorectal manometry increased on day 3, day 7 and day 14 after treatment (P<0.05), and the myoelectric activity of the external anal sphincter increased on day 7 and day 14 after treatment (P<0.05). ④Compared with the electrical stimulation group, the anorectal manometry in the electroacupuncture group was higher than that in the electrical stimulation group on day 3 and day 14 (P<0.05), and the myoelectric activity of the external anal sphincter was stronger than that in the electrical stimulation group on day 7 and day 14 (P<0.05). ⑤The muscle fibers of the external anal sphincter of rats in the blank group were arranged densely and orderly. In the model group, the muscle fiber structure was disordered, the muscle fiber space was widened, and the external anal sphincter atrophy occurred to varying degrees. The structure and arrangement of muscle fibers in electrical stimulation group and electroacupuncture group were significantly improved, and the improvement in electroacupuncture group was more significant.  Conclusion:Electroacupuncture at Zusanli and Changqiang points can effectively improve the fecal incontinence of neurogenic fecal incontinence rats, and its mechanism may be related to increasing anorectal manometry and enhancing myoelectrical activity of external anal sphincter.