Objective:To investigate the effect of Wenyang Sanyu Pingchuan recipe on the levels of nuclear factor-κB (NF-κB) p65, signal transducers and activators of transcription 6 (STAT6) and related inflammatory factors in asthmatic mice. MethodsForty male BALB/c mice were randomly divided into blank control group, asthma model group, dexamethasone group and Wenyang Sanyu Pingchuan recipe group, with 10 mice in each group. Except the blank control group, mice in other groups established the asthma model, and were given corresponding drug intervention. HE staining was used to observe the histopathological changes of the lung, the levels of Interleukin 4 (IL-4), Interleukin 13 (IL-13), interferon-γ (IFN-γ) and Immunoglobulin E (IgE) were determined by ELISA, and the protein levels of p65 and STAT6 were detected by Western blot after 28 days.  Results:Compared with the blank control group, the levels of IL-4, IL-13 and p65, STAT6 in the model group increased significantly (P<0.01), and the level of IFN-γ significantly decreased (P<0.01). Compared with model group, the levels of IL-4 and IL-13 in dexamethasone group and Wenyang Sanyu Pingchuan recipe group, the levels of p65 and STAT6 in dexamethasone group and the level of STAT6 in Wenyang Sanyu Pingchuan recipe group significantly reduced (P<0.01). Compared with model group, the levels of IFN-γ in two drug delivery groups significantly elevated (P<0.01), and the level of p65 in Wenyang Sanyu Pingchuan recipe group reduced (P<0.05). Compared with the dexamethasone group, the level of IL-4 in Wenyang Sanyu Pingchuan recipe group increased (P<0.05), the level of IL-13 significantly increased (P<0.01), and the level of IFN-γ significantly decreased (P<0.01 ), but there was no significant difference in the levels of p65 and STAT6 between dexamethasone group and Wenyang Sanyu Pingchuan recipe group (P>0.05).  Conclusion:Wenyang Sanyu Pingchuan Recipe could reduce the levels of p65 and STAT6 in lung tissue, thus affecting the secretion of inflammatory factors IL-4, IL-13 and IFN-γ in lung. Wenyang Sanyu Pingchuan recipe may relieve airway inflammation in asthma model mice by regulating NF-κB and Janus kinase/signal transduction and transcription activator (JAK/STAT) signaling pathways.