Quality control of Erkeling syrup

FU Dongning; SHAO Jie

doi:10.16305/j.1007-1334.2020.04.015

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Quality control of Erkeling syrup

更新时间：2022-08-31

- Quality control of Erkeling syrup
- Shanghai Journal of Traditional Chinese Medicine Vol. 54, Issue 4, Pages: 97-101(2020)
- 作者机构：
  
  1. 南京中医药大学附属连云港市中医院药学部,江苏,连云港,222004
  2. 江苏康缘药业股份有限公司,江苏,连云港,222001
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2020.04.015
  CLC：
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FU Dongning, SHAO Jie. Quality control of Erkeling syrup. [J]. Shanghai Journal of Traditional Chinese Medicine 54(4):97-101(2020)
DOI：

FU Dongning, SHAO Jie. Quality control of Erkeling syrup. [J]. Shanghai Journal of Traditional Chinese Medicine 54(4):97-101(2020) DOI： 10.16305/j.1007-1334.2020.04.015.

摘要

目的：修订完善儿咳灵糖浆的质量标准。方法：采用TLC鉴别浙贝母；采用HPLC测定盐酸麻黄碱、苦杏仁苷和黄芩苷的含量,色谱柱为Kromasil C,18,柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1% 磷酸溶液,梯度洗脱,流速为1.0 mL/min,检测波长为210 nm(盐酸麻黄碱、苦杏仁苷)、280 nm(黄芩苷),柱温30 ℃。结果：浙贝母的TLC鉴别法专属性强,色谱斑点清晰,阴性对照无干扰。盐酸麻黄碱在1.050～10.500 mg/L范围内与峰面积线性关系良好(r=0.999 4),平均加样回收率为 99.1%(RSD=1.50%,n=9)；苦杏仁苷在5.220～52.200 mg/L范围内与峰面积线性关系良好(r=0.999 0),平均加样回收率为 98.6%(RSD=0.81%,n=9)；黄芩苷在20.050～200.500 mg/L范围内与峰面积线性关系良好(r=0.999 5),平均加样回收率为 98.4%(RSD=0.94%,n=9)。结论：该方法准确、重复性好,可作为儿咳灵糖浆的质量控制标准。

Abstract

Objective:To improve the quality standard of Erkeling syrup. MethodsBulbus Fritillariae Thunbergii was identified by TLC.The contents of ephedrine hydrochloride,amygdalin and baicalin were analyzed by HPLC on Kromasil C,18, column (250 mm × 4.6 mm,5 μm) with the mobile phase of acetonitrile-0.1% phosphate by gradient elution.The flow rate was 1.0 mL/min and the detection wavelength was set at 210 nm for ephedrine hydrochloride and amygdalin,280 nm for baicalin. The column temperature was 30 ℃. RseultsTLC identification method of Bulbus Fritillaria thunbergii had strong specificity. The spots in TLC were clear, and the negative samples showed no interference. Ephedrine hydrochloride had a good linear relationship with peak area in the range of 1.050 to 10.500 mg/L (r=0.999 4), and the average recovery rate was 99.1%(RSD=1.50%, n=9). In the range of 5.220-52.20mg/L, amygdalin had a good linear relationship with peak area (r=0.999 0), and the average recovery rate was 98.6%(RSD=0.81%, n=9). Baicalin showed a good linear relationship with peak area in the range of 20.050-200.500 mg/L (r=0.999 5), with an average sample recovery of 98.4%(RSD=0.94%, n=9). Conclusion:The method is accurate and repeatable,and can be used as the quality control of Erkeling syrup.

关键词

儿咳灵糖浆盐酸麻黄碱苦杏仁苷黄芩苷TLCHPLC

Keywords

Erkeling syrupephedrine hydrochlorideamygdalinbaicalinTLCHPLC

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