Optimization of processing technology of charring chrysanthemum by orthogonal design

GE Xiuyun; DAI Guangxiu

doi:10.16305/j.1007-1334.2019.12.020

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Optimization of processing technology of charring chrysanthemum by orthogonal design

更新时间：2022-08-31

- Optimization of processing technology of charring chrysanthemum by orthogonal design
- Shanghai Journal of Traditional Chinese Medicine Vol. 53, Issue 12, Pages: 78-82(2019)
- 作者机构：
  
  1. 山东省中医药研究院中药炮制研究室,山东,济南,250014
  2. 山东省临沂市中医医院药剂科,山东,临沂,276002
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2019.12.020
  CLC：
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GE Xiuyun, DAI Guangxiu. Optimization of processing technology of charring chrysanthemum by orthogonal design. [J]. Shanghai Journal of Traditional Chinese Medicine 53(12):78-82(2019)
DOI：

GE Xiuyun, DAI Guangxiu. Optimization of processing technology of charring chrysanthemum by orthogonal design. [J]. Shanghai Journal of Traditional Chinese Medicine 53(12):78-82(2019) DOI： 10.16305/j.1007-1334.2019.12.020.

摘要

目的：优选菊花炒炭的最佳工艺。方法：通过L,9,(3,4,)正交试验设计法制备菊花炭样品,采用高效液相色谱法测定绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸的含量,采用Diamonsil C,18,色谱柱(250 mm×4.6 mm,5 μm),以乙腈为流动相A和0.1%磷酸水溶液为流动相B进行梯度洗脱；流速为1.0 mL/min,检测波长为348 nm,柱温为30 ℃。并进行外观性状鉴别、水分和浸出物含量测定。以外观性状、浸出物含量和绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸的含量综合评分,优选菊花炭的最佳炮制工艺和具体工艺参数。结果：菊花的最佳炒炭工艺为:取净制菊花1 000 g,置300 ℃热锅内,翻炒6 min。结论：通过此法优选得到的菊花炒炭的工艺稳定、技术参数明确、可行性高。

Abstract

Objective:To optimize the processing technology of charring chrysanthemum. MethodsCharred Chrysanthemum samples were prepared by means of L,9, (3,4,) orthogonal test design, and the contents of chlorogenic acid, luteolin, 3,5-O-di-coffee acyl quinic acid were determined by high performance liquid chromatography(HPLC). The gradient elution was carried out with acetonitrile as mobile phase A and 0.1% aqueous phosphoric acid as mobile phase B. The flow rate was 1.0 mL/ min, the detection wavelength was 348 nm, and the column temperature was 30 ℃. And the appearance character identification, the water content and the extract content determination were carried out. The optimum processing technology and the specific process parameters of charring chrysanthemum were selected based on the appearance character, the extract content and the content of the chlorogenic acid, the luteolin, the 3,5-O-di-coffee and the quinine acid. ResultThe optimum charring process of the chrysanthemum was as follows:1 000 g of chrysanthemum put in a hot pot with a temperature of 300 ℃, fried for 6 minutes. Conclusion:The optimized technology is stable,feasible and the technical paramater is definite.

关键词

菊花炭正交试验高效液相色谱法绿原酸木犀草苷35-O-二咖啡酰基奎宁酸

Keywords

charried Chrysanthemumorthogonal experimentHPLCchlorogenic acidluteolin35-O-di-coffee acyl quinic acid

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