Objective:ObjectiveTo investigate the effects of Qinggan Yishen Qufeng formula (QYQ) on the dual pathological conditions of myocardial injury and high blood pressure as well as the possible mechanism. MethodsSpontaneously hypertensive rats(SHRs)were randomly divided into QYQ-treated group(QYQ),Candesartan-treated group(Can)as well as the vehicle-treated groups(SHR). Eight male WKY rats of the same age were used as normal control group (WKY).All experimental animals were subject to drug intervention at 6 weeks of age for 14 weeks. The systolic and diastolic blood pressure levels were measured by the tail pressure method at every 2 weeks in each group to evaluate the effect of QYQ. At the age of 20 weeks, the cardiac tissue was taken and paraffin embedded section was performed. HE staining was used to calculate the cross-sectional area of the cardiac muscle cells to evaluate the preventive effect of QYQ on the hypertension-associated myocardial injury. The differential expression of miR-125a-5p and its target genes, such as IL-6R, STAT3 and IL-6 mRNA in cardiac tissue was further tested.  Results:Compared with the model group, QYQ significantly decreased the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of SHR rats, decreased the cross section area of cardiomyocytes (P<0.05), upregulated the expression of miR-125a-5p in cardiac tissue, down-regulated the expression of direct target gene, such as IL-6R,STAT3 mRNA, and down-regulated the expression of IL-6 mRNA in cardiac tissue (P<0.05).  Conclusion:QYQ not only has significant antihypertensive effect, but also has significant preventive effect on hypertension-associated myocardial injury. The regulatory effect of QYQ on miR-125a-5p/IL-6R/STAT3 pathway may be one of the potential molecular mechanisms of QYQ in the prevention and treatment of hypertension-associated myocardial injury.