Objective:To study the diversity of gut microbiome in pancreatic cancer patients with damp-heat syndrome based on high-throughput sequencing technology. This study aims to exploring the role of gut microbiome in the pathogenesis of pancreatic cancer,and providing new ideas for clinical diagnosis and treatment. MethodsThirty-one cases of pancreatic cancer patients with damp-heat syndrome who were treated in our hospital from January 2018 to December 2018 were served as observation group. Another 8 cases of normal people who received a physical examination at the same time were served as control group. Stool samples were collected from two groups of people. DNAs were extracted and the database was constructed. The high-throughput sequencing was performed on the Illumina Hiseq PE150 platform,and then the biological information of the samples was obtained. The species annotation and functional annotation of the samples between the two groups were analyzed and compared.  Results:① After deredundancy, 328 787 mitochondrial open reading frames (ORFs) were obtained. The total number of genes in the two groups was 137 849, the unique gene of the observation group was 114 439, and the unique gene in the control group was 60 473.② There was a significant difference in the species abundance between the two groups at the level of the door and the level of the genus. On the level of the door, the abundance of the flora in the observation group was observed, such as the abundance of Proteobacteria, Acinetobacteria, Verrucomicrobia, Synergistertes and Fibrobacteres, which was higher than that of the control group. But the abundance of Bacteroidetes, Firmicutes, Spirochaetes, Candidatus and Chlamydiae was lower than that of the control group. At the genus level, the abundance of Akkermansia, Collinsella, Streptococcus, Bilophila was higher than that of the control group, while the abundance of Bifidobacterium, Prevotella, Bacteroides, Faecalibacterium, Clostridium, Roseburia was lower than that of the control group.③ The principal coordinate analysis (PCoA) based on distance matrix showed that there was significant difference in the composition and structure of flora between the two groups.④ The results of functional interpretation and analysis of KEGG database showed that the gene expression level in metabolism, environmental information processing and cell processing related pathways in the observation group was higher than that in the control group, and the distribution of functional abundance between the observation group and the control group could be distinguished obviously.  Conclusion:Compared with normal people,the intestinal flora of pancreatic cancer patients with damp-heat syndrome has obvious differences in diversity,flora structure and function,so monitoring the changes of intestinal flora can provide valuable information for clinical prevention and treatment of pancreatic cancer.