Study on quality control standards for Shaoshang Zhentong ointment 

WANG Aili; ZHU Jianyong; ZHANG Chunyan; JIA Qing; CHEN Yu; XU Shun

doi:10.16305/j.1007-1334.2019.11.018

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Study on quality control standards for Shaoshang Zhentong ointment 

更新时间：2022-08-31

- Study on quality control standards for Shaoshang Zhentong ointment 
- Shanghai Journal of Traditional Chinese Medicine Vol. 53, Issue 11, Pages: 86-91(2019)
- 作者机构：
  
  1. 上海中医药大学附属第七人民医院烧伤整形科,上海,200137
  2. 上海中医药大学附属第七人民医院中心实验室,上海,200137
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2019.11.018
  CLC：
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WANG Aili, ZHU Jianyong, ZHANG Chunyan, et al. Study on quality control standards for Shaoshang Zhentong ointment . [J]. Shanghai Journal of Traditional Chinese Medicine 53(11):86-91(2019)
DOI：

WANG Aili, ZHU Jianyong, ZHANG Chunyan, et al. Study on quality control standards for Shaoshang Zhentong ointment . [J]. Shanghai Journal of Traditional Chinese Medicine 53(11):86-91(2019) DOI： 10.16305/j.1007-1334.2019.11.018.

摘要

目的：建立烧伤镇痛油膏(主要为其中的蒲黄、黄芩、丹参、白芷)的质量控制方法。方法：采用薄层色谱法(TLC)对该复方油膏中蒲黄、黄芩、丹参、白芷进行定性鉴别,同时采用高效液相色谱法(HPLC)测定黄芩苷、黄芩素、木犀草素的含量。结果：TLC 鉴别分离度良好,专属性强,阴性样品无干扰；黄芩苷对照品在0.52~13.0 μg(r=0.999 9)范围内线性关系良好,加样回收率均值为100.3%,RSD为2.6%；木犀草素对照品在0.13~3.3 μg(r=0.999 9)范围内线性关系良好,加样回收率均值为103.8%,RSD为2.9%；黄芩素对照品在0.05~1.2 μg(r=0.999 9)范围内线性关系良好,加样回收率均值为103.6%,RSD为2.8%。结论：该方法操作简便易行,专属性强,准确可靠,可用于烧伤镇痛油膏的质量控制。

Abstract

Objective:To establish the quality control standards for Shaoshang Zhentong ointment (Puhuang, Liujinu, Huangqin, Danshen and Baizhi). MethodsPuhuang, Huangqin, Danshen and Baizhi in this compound ointment were identified qualitatively by thin layer chromatography (TLC). The content of baicalin, luteolin and baicalein were determined by high-performance liquid chromatography (HPLC). Results:In identification by TLC, there was good isolation and specificity, without interference from the negative samples. Baicalin, luteolin and baicalein had a good linearity in the range of 0.52~13.0 μg (r=0.999 9), 0.13~3.3 μg (r=0.999 9) and 0.05~1.2 μg (r=0.999 9), respectively. The mean average recovery of baicalin, luteolin and baicalein was 100.3% (RSD=2.6%), 103.8% (RSD=2.9%), and 103.6% (RSD=2.8%), respectively. Conclusion:This method is easy to operate, specific, accurate and reliable, and it can be used for quality control of Shaoshang Zhentong ointment.

关键词

烧伤镇痛油膏薄层色谱高效液相色谱黄芩苷黄芩素木犀草素

Keywords

Shaoshang Zhentong ointmentTLCHPLCbaicalinluteolinbaicalein

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