Objective:To detect the expression levels of angiogenic tumor markers, hypoxia inducible factor-1α(HIF-1α) and vascular endothelial growth factor (VEGF) in tumor tissue of colorectal cancer patients with blood stasis syndrome or non-blood stasis syndrome, and analysis the relationship between blood stasis syndrome and tumor angiogenesis. MethodsOne hundred and sixteen patients (retrospective cases) were divided into the blood stasis syndrome group and non-blood stasis syndrome group. The positive expressions of angiogenic tumor markers, HIF-1α and VEGF in tumor tissue were measured by immunohistochemistry, and combined with clinical stages, the differences on the expressions of above indicators in colorectal cancer patients with blood stasis syndrome or non-blood stasis syndrome were analyzed.  Results:① The clinical stages of colorectal cancer patients with blood stasis syndrome or non-blood stasis syndrome were mainly stage Ⅱa and stage Ⅲb, and there was no statistical significance between the two groups (P>0.05). ② There was statistically significant difference on the positive expression of vascular CD34 in tumor tissue between colorectal cancer patients with blood stasis syndrome and those patients with non-blood stasis syndrome(P＜0.001), but there were no statistically significant differences on the positive expressions of CK-7, CK-20, CEA, CDX-2, p53, Ki-67, nerve S-100 and lymphatic D2-40 (P>0.05). ③ The positive expressions of HIF-1α and VEGF in tumor tissue of colorectal cancer patients with blood stasis syndrome were significantly higher than those of colorectal cancer patients with non-blood stasis syndrome (P<0.001).  Conclusion:Compared with colorectal cancer patients with non-blood stasis syndrome, the positive expressions of CD34, HIF-1α and VEGF in tumor are significantly increased in patients with blood stasis syndrome, which suggests that blood stasis syndrome of colorectal cancer is closely related to tumor angiogenesis.