Effect of honeysuckle on expression of Caspase-3 and NF-κB in mice with viral myocarditis

LOU Xusheng; HU Jinghong; WANG Fen; LI Xiuzhen; LU Weixing

doi:10.16305/j.1007-1334.2019.09.019

您当前的位置：

首页 >

文章列表页 >

Effect of honeysuckle on expression of Caspase-3 and NF-κB in mice with viral myocarditis

更新时间：2022-08-31

- Effect of honeysuckle on expression of Caspase-3 and NF-κB in mice with viral myocarditis
- Shanghai Journal of Traditional Chinese Medicine Vol. 53, Issue 9, Pages: 71-74(2019)
- 作者机构：
  
  1. 北京中医药大学第三附属医院心血管内科,北京,100029
  2. 北京中医药大学中医学院科研实验中心,北京,100029
  3. 北京中医药大学第三附属医院内分泌科,北京,100029
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2019.09.019
  CLC：
扫描看全文
LOU Xusheng, HU Jinghong, WANG Fen, et al. Effect of honeysuckle on expression of Caspase-3 and NF-κB in mice with viral myocarditis. [J]. Shanghai Journal of Traditional Chinese Medicine 53(9):71-74(2019)
DOI：

LOU Xusheng, HU Jinghong, WANG Fen, et al. Effect of honeysuckle on expression of Caspase-3 and NF-κB in mice with viral myocarditis. [J]. Shanghai Journal of Traditional Chinese Medicine 53(9):71-74(2019) DOI： 10.16305/j.1007-1334.2019.09.019.

摘要

目的：探讨中药金银花对病毒性心肌炎小鼠Caspase-3、NF-κB表达的影响。方法：将80只雄性BALB/c小鼠随机分为4组,每组20只,分别为正常对照组、模型组、金银花低剂量组和金银花高剂量组。模型组和金银花高、低剂量组小鼠均腹腔注射柯萨奇病毒B3(CVB3),建立病毒性心肌炎模型。金银花高、低剂量组于注射病毒后30 min开始灌服金银花药液,每日1次,共14 d；模型组以0.9%NaCl溶液灌胃；正常对照组用不含病毒的等量Eagle液腹腔注射,等量的0.9%NaCl溶液灌胃。各组分别在14 d随机抽取15只小鼠处死,HE染色观察心肌病理改变,免疫组化检测心肌组织中Caspase-3、NF-κB的表达。结果：模型组的Caspase-3、NF-κB表达水平均明显高于正常对照组,金银花高、低剂量组的Caspase-3、NF-κB表达较模型组均明显减轻；金银花高剂量组的Caspase-3、NF-κB表达水平较金银花低剂量组减轻。结论：金银花可明显抑制病毒性心肌炎小鼠Caspase-3、NF-κB的表达,为临床应用金银花治疗病毒性心肌炎提供了实验依据。

Abstract

Objective:To investigate the effect of honeysuckle on the expression of Caspase-3 and NF-κB in mice with viral myocarditis. MethodsA total of 80 male BALB/c mice were divided into 4 groups randomly with 20 mice in each group:normal control group,model group,honeysuckle low-dose group and honeysuckle high-dose group. Coxsackievirus B3 (CVB3) was injected intraperitoneally into mice in model group and both dose groups to establish viral myocarditis model. The low and high dose groups were given honeysuckle liquid 30 minutes after virus injection, once a day for 14 days; the model group was given saline by gastric lavage; the control group was given intraperitoneal injection of Equivalent Eagle liquid without virus and normal saline by gastric lavage. Totally 15 mice were randomly selected and executed from each group on the 14th day. The pathological changes of myocardium were observed by HE staining, and the expression of Caspase-3, NF-κB in myocardial tissue was detected by immunohistochemistry. Results:The expression of Caspase-3 and NF-κB in the model group was significantly higher than that in the control group. The expression of Caspase-3 and NF-κB in the two dose groups was significantly lower than that in the model group. The expression of Caspase-3 and NF-κB in the honeysuckle high-dose group was significantly lower than that in the honeysuckle low-dose group. Conclusion:Honeysuckle can significantly inhibit the expression of Caspase-3 and NF-κB in mice with viral myocarditis, which provides the experimental basis for clinical application of honeysuckle in the treatment of viral myocarditis.

关键词

病毒性心肌炎金银花caspase-3NF-κB小鼠

Keywords

myocarditishoneysucklecaspase-3NF-κBmice

references

Views

328

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Common molecular mechanism of aristolochic acid I inducing hepatorenal toxicity

Effects of Qinfei Oral Liquid on airway inflammation and gut microbiota in asthmatic mice

Optimization of active components compatibility of Jiangzhi Granule to improve NAFLD based on weighed modification method

Effect of Bushen Huoxue Formula on expression of VEGF/PI3K/Akt pathway in decidua of mice with recurrent abortion

Experimental study on improving sexual dysfunction of male mice by Aigui Gel Ointment

Related Author

No data

Related Institution

Second Department of Hepatology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Institute of Liver Diseases， Shanghai University of Traditional Chinese Medicine

Shanghai Key Laboratory of Traditional Chinese Clinical Medicine

MOE Key Laboratory of Liver and Kidney Diseases， Shanghai University of Traditional Chinese Medicine

Department of Emergency Medicine， Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

⁰