Changes in tongue color, blood flow of tongue microcirculation and ICAM-1 in diabetic rats

CHEN Qingguang; XU Jiatuo; LU Hao; LI Junyan; XU Junfei; GONG Fan; XU Peiying; HAN Xu; LIU Yahua

doi:10.16305/j.1007-1334.2019.08.018

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Changes in tongue color, blood flow of tongue microcirculation and ICAM-1 in diabetic rats

更新时间：2022-08-31

- Changes in tongue color, blood flow of tongue microcirculation and ICAM-1 in diabetic rats
- Shanghai Journal of Traditional Chinese Medicine Vol. 53, Issue 8, Pages: 72-76(2019)
- 作者机构：
  
  1. 上海中医药大学附属曙光医院,上海,201203
  2. 上海中医药大学基础医学院,上海,201203
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2019.08.018
  CLC：
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CHEN Qingguang, XU Jiatuo, LU Hao, et al. Changes in tongue color, blood flow of tongue microcirculation and ICAM-1 in diabetic rats. [J]. Shanghai Journal of Traditional Chinese Medicine 53(8):72-76(2019)
DOI：

CHEN Qingguang, XU Jiatuo, LU Hao, et al. Changes in tongue color, blood flow of tongue microcirculation and ICAM-1 in diabetic rats. [J]. Shanghai Journal of Traditional Chinese Medicine 53(8):72-76(2019) DOI： 10.16305/j.1007-1334.2019.08.018.

摘要

目的：探讨2型糖尿病大鼠的舌色变化、舌微循环血流及黏附因子ICAM-1的改变。方法：Wistar大鼠除正常组10只外,其余大鼠制作2型糖尿病大鼠模型,造模成功大鼠随机分为模型组、抑制剂组,每组10只。抑制剂组腹腔注射 NF-κB信号通路抑制剂PDTC 8周。采用“TDA-1小型舌象仪”与“舌象数字分析诊断系统(TDAS)”,采集并分析糖尿病大鼠的舌象图像,通过激光多普勒血流仪观察糖尿病大鼠舌局部微循环血流动力学的改变,采用ELISA法观察血清、舌组织、肝组织黏附因子ICAM-1的浓度。结果：舌象客观指标比较:与正常组比较,模型组与抑制剂组舌质颜色定量指标zhiClrI、taiClrI明显降低,zhiClrS、taiClrS明显升高(P<0.05)；与模型组比较,抑制剂组舌质颜色定量指标zhiClrS降低(P<0.05)。舌局部血流动力学比较:与正常组相比,模型组与抑制剂组舌局部微循环血流平均灌注面积均明显减小(P<0.05)。黏附因子比较:与正常组相比,模型组血清黏附因子ICAM-1浓度升高(P<0.05),舌组织与肝组织黏附因子ICAM-1浓度明显升高(P<0.01)；与模型组比较,抑制剂组舌组织与肝组织黏附因子ICAM-1的浓度明显降低(P<0.01)。结论：2型糖尿病大鼠舌色的变化可能与舌局部微循环障碍、黏附因子ICAM-1升高有关。

Abstract

Objective:To explore the changes in tongue color, blood flow of tongue microcirculation and ICAM-1 in rats with type 2 diabetes mellitus. MethodsExcept for the 10 rats in the normal group, the other Wistar rats were used to establish the model of type 2 diabetes mellitus. Rats for successful modelling were randomly assigned into model group and inhibitor group, 10 rats in each group. In the inhibitor group, PDTC, the inhibitor of the NF-κB signaling pathway was intraperitoneally injected for 8 weeks. The tongue images of diabetic rats were collected and analyzed using “small TDA-1 tongue imager” and “tongue digital analysis system (TDAS)”. The changes of hemodynamics in tongue microcirculation of diabetic rats were observed by laser Doppler flowmetry. The concentrations of ICAM-1 in serum, tongue tissue and liver tissue were observed by ELISA. Results:In comparison of objective indicators of tongue images, it was shown that compared with the normal group, zhiClrI and taiClrI (the quantitative indicators of tongue color) were significantly reduced, while zhiClrS and taiClrS (the quantitative indicators of tongue color) were significantly increased in the model group and the inhibitor group (P<0.05). Compared with the model group, zhiClrS (the quantitative indicator of tongue color) was reduced in the inhibitor group (P<0.05). In comparison of local hemodynamics of the tongue, it was shown that compared with the normal group, mean perfusion area of blood flow in local tongue microcirculation decreased significantly in the normal group and the inhibitor group (P<0.05). In comparison of adhesion factor, it was shown that compared with the normal group, the concentration of serum adhesion factor ICAM-1 increased (P<0.05) and the concentration of ICAM-1 in tongue tissue and liver tissue was significantly increased (P<0.01) in the model group. Compared with the model group, the concentration of ICAM-1 in tongue tissue and liver tissue was significantly reduced in the inhibitor group (P<0.01). Conclusion:The changes of tongue color in rats with type 2 diabetes mellitus may be related to disorder of local microcirculation of the tongue and increase of the adhesion factor ICAM-1.

关键词

2型糖尿病舌象客观化微循环障碍黏附因子ICAM-1大鼠

Keywords

type 2 diabetes mellitusobjectification of tongue imagedisorder of microcirculationICAM-1rats

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