Objective:To investigate the protective effects of Qingchang Suppository (QCS) medicated serum on lipopolysaccharide (LPS)-induced Caco-2 cell injury model. MethodsCaco-2 cells in logarithmic growth phase were collected and divided into five groups: normal control group, LPS group, low-dose QCS group (2.5% QCS medicated serum), medium-dose QCS group (5% QCS medicated serum) and high-dose QCS group (10% QCS medicated serum). MTT assay was used to detect cell viability; ELISA was used to determine the content of IL-8, IL-17 and PGE,2, and the levels of MDA, GSH and SOD in the supernatant; scratch assay was employed to test cell migration ability in vitro; Western blot was used to detect the expression of RhoA and Rac1 proteins.  Results:QCS antagonized the decrease in Caco-2 cell viability induced by LPS. Compared with the LPS group, QCS significantly reduced the secretion of IL-8, IL-17 and PGE,2, induced by LPS (P,<,0.05), lowered the release of MDA induced by LPS and increased the secretion of GSH and SOD (P,<,0.01); the scratch width in the QCS group decreased significantly and the cell migration ability increased at 48 h (P,<,0.001), which speeded up the repair of scratches in a concentration-dependent manner; Western blot results showed that QCS could effectively reverse LPS-induced inhibition of RhoA and Rac1 protein expression (P,<,0.05).  Conclusion:Qingchang Suppository medicated serum protects Caco-2 cells from LPS-induced injury and increases cell survival rate by inhibiting inflammatory factors and antioxidant stress and promoting migration of colonic epithelial cells.