Effect of Ganmai Dazao Decoction on BDNF and SERT gene expression in prefrontal lobe and amygdala and on serum level of 5-HT in rats with depression

XU Mingyue; WANG Yu; ZHAO Yan; NI Hongmei; LEI Yuanying; LI Juan

doi:10.16305/j.1007-1334.2019.03.021

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Effect of Ganmai Dazao Decoction on BDNF and SERT gene expression in prefrontal lobe and amygdala and on serum level of 5-HT in rats with depression

更新时间：2022-08-31

- Effect of Ganmai Dazao Decoction on BDNF and SERT gene expression in prefrontal lobe and amygdala and on serum level of 5-HT in rats with depression
- Shanghai Journal of Traditional Chinese Medicine Vol. 53, Issue 3, Pages: 89-92(2019)
- 作者机构：
  
  1. 上海中医药大学基础医学院,上海,201203
  2. 江苏省无锡工人太湖疗养院,江苏无锡,214000
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2019.03.021
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XU Mingyue, WANG Yu, ZHAO Yan, et al. Effect of Ganmai Dazao Decoction on BDNF and SERT gene expression in prefrontal lobe and amygdala and on serum level of 5-HT in rats with depression. [J]. Shanghai Journal of Traditional Chinese Medicine 53(3):89-92(2019)
DOI：

XU Mingyue, WANG Yu, ZHAO Yan, et al. Effect of Ganmai Dazao Decoction on BDNF and SERT gene expression in prefrontal lobe and amygdala and on serum level of 5-HT in rats with depression. [J]. Shanghai Journal of Traditional Chinese Medicine 53(3):89-92(2019) DOI： 10.16305/j.1007-1334.2019.03.021.

摘要

目的：研究甘麦大枣汤对抑郁症模型大鼠前额叶和杏仁核中脑源性神经营养因子(BDNF)、5-羟色胺转运体(SERT)基因表达及血清5-羟色胺(5-HT)水平的影响。方法：将40只Wistar雄性大鼠随机分为正常组、模型组、甘麦大枣汤组和盐酸氟西汀组,每组10只。除正常组外,其余各组以慢性不可预见性温和刺激配合孤养的方法制备抑郁症模型。造模后,甘麦大枣汤组灌胃给予甘麦大枣汤浓缩药液(12.5 ml/kg),盐酸氟西汀组灌胃给予盐酸氟西汀药液(10 ml/kg),正常组和模型组灌胃给予蒸馏水2 ml。各组均干预28天,气相色谱-质谱联用检测血清5-HT水平,实时荧光定量PCR检测大鼠前额叶及杏仁核中BDNF mRNA、SERT mRNA的相对表达量。结果：①与正常组比较,模型组大鼠血清5-HT水平降低(P<0.05),前额叶及杏仁核中BDNF mRNA、SERT mRNA相对表达量减少(P<0.05)。②与模型组比较,甘麦大枣汤组、盐酸氟西汀组大鼠血清5-HT水平升高(P<0.05)；甘麦大枣汤组大鼠前额叶中BDNF mRNA相对表达量增加(P<0.05),盐酸氟西汀组大鼠前额叶中BDNF mRNA和SERT mRNA相对表达量增加(P<0.05)；甘麦大枣汤组、盐酸氟西汀组大鼠杏仁核中BDNF mRNA、SERT mRNA相对表达量增加(P<0.05)。③与盐酸氟西汀组比较,甘麦大枣汤组大鼠血清5-HT水平,前额叶及杏仁核中BDNF mRNA、SERT mRNA的相对表达量差异无统计学意义(P>0.05)。结论：甘麦大枣汤可能通过升高血清5-HT水平、增加前额叶和杏仁核中BDNF mRNA及杏仁核中SERT mRNA的表达,对抑郁症发挥干预作用。

Abstract

Objective:To study the effects of Ganmai Dazao Decoction on the expression of brain-derived neurotrophic factor (BDNF) and serotonin transporter (SERT) genes in prefrontal lobe and amygdala and on the serum level of 5-hydroxytryptamine (5-HT) in depression model rats. Methods40 male Wistar rats were randomly assigned into normal group, model group, Ganmai Dazao Decoction group and fluoxetine hydrochloride group, 10 rats in each group. Except the normal group, the depression model was established by chronic unpredictable mild stress (CUMS) combined with orphaned method in the other groups. After modeling, Ganmai Dazao Decoction concentrate solution (12.5 ml/kg) was given to the Ganmai Dazao Decoction group, fluoxetine hydrochloride solution (10 ml/kg) was administered to the fluoxetine hydrochloride group and 2 ml of distilled water was given to the normal and model groups. All groups were treated for 28 days. The serum level of 5-HT in rats was detected by GC-MS; and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe and amygdala was determined by real-time quantitative PCR. Results:①The serum level of 5-HT and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe and amygdala decreased in the model group compared to the normal group (P<0.05). ②Compared to the model group, the serum level of 5-HT increased in the Ganmai Dazao Decoction and fluoxetine hydrochloride groups (P<0.05); the relative expression of BDNF mRNA in prefrontal lobe increased in the Ganmai Dazao Decoction group (P<0.05) and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe increased in the fluoxetine hydrochloride group (P<0.05); and the relative expression of BDNF mRNA and SERT mRNA in amygdala increased in the Ganmai Dazao Decoction and fluoxetine hydrochloride groups (P<0.05). ③There were no statistically significant differences in the serum level of 5-HT and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe and amygdala between the Ganmai Dazao Decoction group and the fluoxetine hydrochloride group (P>0.05). Conclusion:Ganmai Dazao Decoction may treat depression by increasing the serum level of 5-HT and the expression of BDNF mRNA in prefrontal lobe and amygdala and SERT mRNA in amygdala.

关键词

抑郁症大鼠甘麦大枣汤5-羟色胺脑源性神经营养因子5-羟色胺转运体基因表达

Keywords

depressionratsGanmai Dazao Decoction5-HTBDNFSERTgene expression

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