Objective:To observe the changes of gastrointestinal motility in rats with functional dyspepsia (FD), investigate the difference in the effect of regulating gastrointestinal motility following different acupoint pressure sequences and explore the sequence effect of “first opening Middle Jiao” in zang-fu viscera acupoint pressure therapy. Methods60 SD rats were randomly assigned into the blank group, the model group, the Upper-Middle Jiao group, the Middle-Upper Jiao group and the Middle-Lower Jiao group, 12 rats in each group. Except the blank group, rats in the other groups were treated with mild tail-clamping stimulation plus intermittent diet for 21 days for modeling. Each group was given corresponding intervention for 14 consecutive days after successful modeling. The behavior, electrogastrogram, intragastric residual rate and small intestinal propulsion rate of rats were observed and the expression of c-kit was detected in the gastric antrum tissue in each group.  Results:The gastric electrical amplitude was lower in the model group than in the blank group (P<0.05); it was higher in the Upper-Middle Jiao and Middle-Lower Jiao groups than that in the model group (P<0.05); the gastric residual rate was higher and the small intestinal propulsion rate was lower in the model group than in the blank group (P<0.05); the gastric residual rate was lower and the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the model group (P<0.05); and in the manipulation intervention groups, the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the Upper-Middle Jiao and Middle-Upper Jiao groups (P<0.05). The mean optical density (OD) value of c-kit in the gastric antrum tissue was lower in the model group than in the blank group (P<0.01), with decrease in c-kit positive interstitial cells of Cajal (ICCs) and a lighter color. The mean OD value was higher in the Middle-Lower Jiao group than in the model group (P<0.05), with increase in c-kit positive ICCs and a darker color.  Conclusion:Zang-fu viscera acupoint pressure therapy can effectively treat gastrointestinal motility disorder in FD rats, upregulating the expresion of c-kit and “first opening Middle Jiao” in the Middle-Lower Jiao group shows the best effect.