Study on cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish

ZHAO Hui; LIU Lian; FEI Fei; WANG Lei; ZHOU Duan; WANG Xu; WANG Youhua

doi:10.16305/j.1007-1334.2019.02.019

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Study on cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish

更新时间：2022-08-31

- Study on cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish
- Shanghai Journal of Traditional Chinese Medicine Vol. 53, Issue 2, Pages: 72-76(2019)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院心内科,上海,200032
  2. 复旦大学附属儿科医院心内科,上海,201102
  3. 复旦大学基础医学院生物化学与分子生物学系,上海,200032
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2019.02.019
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ZHAO Hui, LIU Lian, FEI Fei, et al. Study on cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish. [J]. Shanghai Journal of Traditional Chinese Medicine 53(2):72-76(2019)
DOI：

ZHAO Hui, LIU Lian, FEI Fei, et al. Study on cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish. [J]. Shanghai Journal of Traditional Chinese Medicine 53(2):72-76(2019) DOI： 10.16305/j.1007-1334.2019.02.019.

摘要

目的：观察扩心方对与扩张型心肌病相似表型的tnnt2a突变斑马鱼模型的心脏保护作用,并探讨其可能的作用机制。方法：以正常野生型斑马鱼作为对照组,tnnt2a突变斑马鱼作为模型组,tnnt2a突变斑马鱼给予5 μg/ml的扩心方进行干预作为扩心方组。研究扩心方对tnnt2a突变斑马鱼的作用,观察生存率、静脉窦-动脉球(SV-BA)距离、病理切片HE染色、ATP含量、凋亡相关基因bcl-2/bax的表达情况。结果：与模型组比较,扩心方对tnnt2a突变斑马鱼的心脏具有保护作用,可以提高模型组斑马鱼的生存率,增加ATP的含量,增加bcl-2/bax基因的表达(P<0.05,P<0.01)。结论：扩心方对于tnnt2a突变斑马鱼模型具有心脏保护作用,其机制可能与改善能量代谢和减少心肌细胞的凋亡有关。

Abstract

Objective:To observe the cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish model with similar phenotype to dilated cardiomyopathy and explore the possible mechanism of action. MethodsThe normal wild-type zebrafish were used in the control group, the tnnt2a mutant zebrafish were used in the model group and the tnnt2a mutant zebrafish that received Kuoxinfang 5 μg/ml were used in the Kuoxinfang group. The effect of Kuoxinfang on tnnt2a mutant zebrafish was studied and the survival rate, sinus venosus-bulbus arteriosus (SV-BA) distance, HE staining of pathological sections, ATP content and expression of the apoptosis-related gene bcl-2/bax were observed. Results:Compared to the model group, Kuoxinfang has protective effect on the heart of tnnt2a mutant zebrafish, as it improves the survival rate, increases the ATP content and upregulates expression of the bcl-2/bax gene in zebrafish of the model group (P<0.05, P<0.01). Conclusion:Kuoxinfang shows cardioprotective effect in the tnnt2a mutant zebrafish model, and the mechanism may be associated with improvement in energy metabolism and reduction in apoptosis of cardiomyocytes.

关键词

扩张型心肌病斑马鱼扩心方心脏保护

Keywords

dilated cardiomyopathyzebrafishKuoxinfangcardioprotection

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