Anti-inflammatory and analgesic effects of Daphnis Cortex enteric-coated preparations on adjuvant arthritis rats

CHENG Wenhao; ZHANG Tianrui; LI Yinghui; MA Dan; SONG Jingyi; ZHANG Zhongyi; ZHANG Shuofeng

doi:10.16305/j.1007-1334.2019.01.025

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Anti-inflammatory and analgesic effects of Daphnis Cortex enteric-coated preparations on adjuvant arthritis rats

更新时间：2022-08-31

- Anti-inflammatory and analgesic effects of Daphnis Cortex enteric-coated preparations on adjuvant arthritis rats
- Shanghai Journal of Traditional Chinese Medicine Vol. 53, Issue 1, Pages: 101-105(2019)
- 作者机构：
  
  1. 北京中医药大学中药学院,北京,100029
  2. 山海关药业有限责任公司,河北,秦皇岛,066000
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2019.01.025
  CLC：
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CHENG Wenhao, ZHANG Tianrui, LI Yinghui, et al. Anti-inflammatory and analgesic effects of Daphnis Cortex enteric-coated preparations on adjuvant arthritis rats. [J]. Shanghai Journal of Traditional Chinese Medicine 53(1):101-105(2019)
DOI：

CHENG Wenhao, ZHANG Tianrui, LI Yinghui, et al. Anti-inflammatory and analgesic effects of Daphnis Cortex enteric-coated preparations on adjuvant arthritis rats. [J]. Shanghai Journal of Traditional Chinese Medicine 53(1):101-105(2019) DOI： 10.16305/j.1007-1334.2019.01.025.

摘要

目的：探讨祖师麻肠溶制剂对佐剂性关节炎大鼠的抗炎与镇痛作用。方法：右后肢足跖皮内注射弗氏完全佐剂制备佐剂性关节炎大鼠模型。将大鼠分为对照组、模型组、泼尼松组,及中药低、中、高剂量组,每组12只。泼尼松组灌胃以泼尼松3.2 mg/kg,中药低、中、高剂量组分别灌胃以祖师麻肠溶制剂0.396、0.792、1.584 g/kg,对照组、模型组灌胃以等体积的7.5%CMC-Na溶液。各组均连续干预12 d,于造模后第7 d、第12 d计算大鼠足跖肿胀率、50%机械缩足反射阈值(MWT)、热缩足反射阈值(TWT)；末次给药24 h后ELISA法测定大鼠血清白介素-6(IL-6)、前列腺素E,2,(PGE,2,)、肿瘤坏死因子-α(TNF-α)水平,HE染色观察大鼠膝关节和踝关节病理学改变。结果：①与对照组比较,模型组大鼠足跖肿胀率升高(P,<,0.01),50%MWT、TWT降低(P,<,0.01),血清IL-6、PGE,2,、TNF-α水平升高(P,<,0.01)。②=2\*GB3与模型组比较,泼尼松组和中药低、中、高剂量组大鼠足跖肿胀率降低(P,<,0.01),50%MWT、TWT升高(P,<,0.01),血清IL-6、PGE,2,、TNF-α水平降低(P,<,0.01)。③=3\*GB3泼尼松组与中药各剂量组比较、中药各剂量组组间比较,上述指标差异无统计学意义(P,>,0.05)。结论：祖师麻肠溶制剂具有明显的抗炎、镇痛作用,可明显改善佐剂性关节炎大鼠膝关节及踝关节病理损伤。

Abstract

Objective:To investigate the anti-inflammatory and analgesic effects of Daphnis Cortex enteric-coated preparation on adjuvant arthritis rats. MethodsThe rat model of adjuvant arthritis was established by intradermal injection of Freund’s complete adjuvant. The rats were divided into the control group,model group,prednisone group,and low,middle and high dose groups of Chinese medicine, 12 rats in each group. The prednisone group was given prednisone 3.2 mg/kg by gavage,and the low,middle and high dose groups of Chinese medicine were given the medicine 0.396 g/kg,0.792g/kg,1.584 g / kg,respectively by gavage. The control group and the model group were given the same volume of 7.5% CMC-Na solution. After continuous intervention for 12 days,the swelling rate of voix pedis,the 50% mechanical withdrawal threshold (MWT) and thermal withdrawal threshold (TWT) were calculated on the 7th and 12th day after modeling. The serum levels of interleukin-6 (IL-6),prostaglandin E,2, (PGE,2,) and tumor necrosis factor- α (TNF- α) were measured by ELISA 24 hours after the last administration. The pathological changes were observed by HE staining in the knee and ankle joints of the rats. Results:①Compared with the control group,the swelling rate of voix pedis in the model group was higher than that in the model group (P,<,0.01) and the value of 50%MWT,TWT was decreased (P,<,0.01) but the levels of IL-6,PGE,2,TNF- α in serum were decreased (P,<,0.01). ② Compared with the model group,the swelling rate of voix pedis was decreased (P,<,0.01),the 50% MWT and TWT were increased (P,<,0.01),and the levels of the serum IL-6,PGE,2,TNF- α were decreased in the prednisone group,and in the low,middle and high dose groups of Chinese medicine (P,<,0.01). ③ There was no significant difference among prednisone group and Chinese medicine groups in the above indicators (P,>,0.05). Conclusion:Daphne Cortex enteric-coated preparation has obvious anti-inflammatory and analgesic effects. It can significantly improve the pathological damage of knee joint and ankle joint in rats with adjuvant arthritis.

关键词

祖师麻佐剂性关节炎大鼠抗炎镇痛

Keywords

Daphnes Cortexadjuvant arthritisratanti-inflammatoryanalgesic

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