Influence of alkaline hydrolysis conditions on contents of 4 rare ginsenosides

ZHANG Weiyun; LIU Fagui; ZHENG Yinan

doi:10.16305/j.1007-1334.2018.12.025

您当前的位置：

首页 >

文章列表页 >

Influence of alkaline hydrolysis conditions on contents of 4 rare ginsenosides

更新时间：2022-08-31

- Influence of alkaline hydrolysis conditions on contents of 4 rare ginsenosides
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 12, Pages: 101-104(2018)
- 作者机构：
  
  1. 福建省中药精加工与健康产品开发重点研究室,厦门医学院药学系,福建,厦门,361023
  2. 吉林农业大学中药材学院,吉林,长春,130118
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.12.025
  CLC：
扫描看全文
ZHANG Weiyun, LIU Fagui, ZHENG Yinan. Influence of alkaline hydrolysis conditions on contents of 4 rare ginsenosides. [J]. Shanghai Journal of Traditional Chinese Medicine 52(12):101-104(2018)
DOI：

ZHANG Weiyun, LIU Fagui, ZHENG Yinan. Influence of alkaline hydrolysis conditions on contents of 4 rare ginsenosides. [J]. Shanghai Journal of Traditional Chinese Medicine 52(12):101-104(2018) DOI： 10.16305/j.1007-1334.2018.12.025.

摘要

目的：探讨不同碱水解条件对4种稀有人参皂苷20(S)-Rh,1,、20(S)-Rh,2,、20(S)-PPt、20(S)-PPd含量的影响。方法：应用高效液相色谱-质谱联用方法(HPLC-MS)初步推断人参茎叶总皂苷碱水解生成的人参皂苷结构,用高效液相色谱法(HPLC)测定不同碱水解反应时间、反应温度、氢氧化钠(NaOH)加入量对人参皂苷20(S)-Rh,1,、20(S)-Rh,2,、20(S)-PPt、20(S)-PPd含量的影响。结果：碱水解40 min、反应温度分别为200 ℃和220 ℃、加入NaOH 2.0 g,有利于获得人参皂苷20(S)-Rh,1,和20(S)-Rh,2,；碱水解6 h、反应温度设置为240 ℃、加入NaOH 2.0 g,有利于获得人参皂苷20(S)-PPt和20(S)-PPd。结论：人参皂苷20(S)-Rh,1,、20(S)-Rh,2,和人参皂苷20(S)-PPt、20(S)-PPd的含量受碱水解反应温度、NaOH加入量的影响趋势基本相同,但受碱水解反应时间的影响趋势不同。

Abstract

Objective:To investigate the influence of different alkaline hydrolysis conditions on the contents of 4 rare ginsenosides including 20(S)-Rh,1, 20(S)-Rh,2, 20(S)-PPt, and 20(S)-PPd. MethodsHigh performance liquid chromatography-mass spectrometry (HPLC-MS) was applied to preliminarily infer the ginsenoside structure generated by alkaline hydrolysis of total saponin of ginseng stem and leaf. HPLC was adopted to determine the influence of different alkaline hydrolysis conditions, including reaction time, reaction temperature, amount of sodium hydroxide (NaOH), on the contents of ginsenosides 20(S)-Rh,1, 20(S)-Rh,2, 20(S)-PPt and 20(S)-PPd. Results:To obtain the high yield of ginsenoside 20(S)-Rh,1, and 20(S)-Rh,2, the condition was suggested as alkaline hydrolysis for 40 minutes, reaction temperature at 200 and 220 ℃ respectively and adding 2.0 g NaOH. To obtain the high yield of ginsenoside 20(S)-PPt and 20(S)-PPd, the condition was suggested as alkaline hydrolysis for 6 hours, reaction temperature at 240 ℃ and adding 2.0 g NaOH. Conclusion:The influence trend of reaction temperature in alkaline hydrolysis and amount of NaOH on the contents of ginsenoside 20(S)-Rh,1, 20(S)-Rh,2, 20(S)-PPt and 20(S)-PPd was similar, while the influence trend of reaction time was different.

关键词

稀有人参皂苷碱水解人参皂苷20(S)-Rh12人参皂苷20(S)-PPt人参皂苷20(S)-PPd

Keywords

rare ginsenosidealkaline hydrolysisginsenoside 20(S)-Rh12ginsenoside 20(S)-PPtginsenoside 20(S)-PPd

references

Views

327

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Advances on effects of ginsenoside Rg₂ and ginsenoside Rh₁ on nervous system and related mechanisms

Effects of Zhuyu Quzhuo Decoction on lipoprotein associated phospholipase A₂ level in patients of stable angina pectoris of coronary heart disease combined with hyperlipidemia

Clinical observation on effect of extracorporeal shock wave acupoint therapy on shoulder pain and levels of serum 5-HT and PGE₂ in patients with shoulder periarthritis

Experimental study of effects of Qinma Formula on airway remodeling in rats with chronic obstructive pulmonary disease

The influence of “Penyan Fang” on the TGF-β₁ and TβRⅡ expression of the sequelae of pelvic inflammatory disease rats’ ovary and uterus

Related Author

No data

Related Institution

College of Chinese Medicinal Materials, Jilin Agricultural University

Retired Cardres Health Center, Combined Service Force Department of Nanjing Military Area Command

Changhai Hospital Affiliated to the Second Military Medical University

Department of Geriatrics, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

School of Basic Medical Science, Shanghai University of Traditional Chinese Medicine

⁰