Objective:To observe the effects of Tongjiang Mixture on rat model of simple gastroesophageal reflux and the influence on brain-gut axis. MethodsThe rat model of simple gastroesophageal reflux was established by surgery in healthy male SD rats. 30 successfully modeling rats were randomly divided into the model group and Tongjiang Mixture groups with low- and high-dose, 10 rats in each group. 10 healthy male SD rats were taken as the control group. The control group was only treated with suture after opening abdominal cavity. The Tongjiang Mixture groups with low- and high-dose were treated with Tongjiang Mixture at dose of 10 ml/kg and 20 ml/kg by intragastric administration, and the control group and model group were treated with 0.9% NaCl solution at dose of 20 ml/kg, with a course of 4 continuous weeks. The electrogastrogram (EGG) was observed. The pH value of mucous membrane of lower esophagus and gastric juice and the level of pepsin in gastric juice were detected. The levels of gastrointestinal active substances in rat plasma and gastric antrum tissue were measured by ELISA. The levels of neurotransmitter in rat plasma were detected by HPLC. The esophagus was isolated for esophagitis evaluation.  Results:①Compared with the control group, the frequency and amplitude of EGG, the pH value of mucous membrane of lower esophagus, the gastrin level in plasma and the levels of gastrointestinal active substances (gastrin, motilin and substance P) in gastric antrum tissue were decreased in the model group (P<0.05), the levels of pepsin in gastric juice and neurotransmitter (norepinephrine, 5-hydroxytryptamine and dopamine) in plasma and the esophagitis score were increased (P<0.05). ②Compared with the model group, the frequency and amplitude of EGG in the Tongjiang Mixture groups with low- and high-dose were increased (P<0.05), and the pH value of mucous membrane of lower esophagus was increased (P<0.05). ③Compared with the model group, the gastrin levels in rat plasma and gastric antrum tissue were increased in the Tongjiang Mixture groups with low- and high-dose (P<0.05), and the plasma levels of norepinephrine and dopamine were decreased in the Tongjiang Mixture group with high-dose (P<0.05). ④Compared with the model group, the esophagitis score of rats in the Tongjiang Mixture groups with low- and high-dose was reduced (P<0.05).  Conclusion:Tongjiang Mixture can obviously improve the gastrointestinal motility and inhibit the regurgitation injury in rat model of simple gastroesophageal reflux, and regulate the brain-gut axis related peripheral neurotransmitters and gastrointestinal active substances.