Objective:To investigate the preventive and therapeutic effects of “Zhi San Zhen” acupuncture on liver injury induced by high intensity training in rats. MethodsSD rats aged 6-8 weeks were randomly divided into the normal group, model group and treatment group. The normal group was treated with routine feeding. The model group was treated with high intensity exercise at intensity of 60 minutes everyday, once a day, six times a week for 8 weeks. Based on the high intensity exercise, the treatment group was treated with “Zhi San Zhen” acupuncture once a day. The general survival state and body weight of rats in each group were observed. The protein content in the liver was detected by Coomassie blue staining, the liver glycogen content in the liver was detected by colorimetric assay, the superoxide dismutase(SOD)activity in the liver was determined by xanthine oxidase method, and the MDA content in the liver was detected by TBA method. The histopathological changes of liver were observed under light microscope, and the ultrastructural changes of liver were examined by transmission electron microscopy.  Results:Compared with the model group, the general survival state of rats was improved in the treatment group and the body weight was increased in varying degrees. The protein content, liver glycogen level and liver SOD activity were obviously increased in the treatment group (P<0.05, P<0.01), and the MDA level was significantly decreased (P<0.01). The results of pathology and electron microscopy showed that the damage of hepatocyte was improved in the treatment group.  Conclusion:“Zhi San Zhen” acupuncture can alleviate the liver injury caused by long-term high intensity training in rats and exert the protective effects on liver injury. The main mechanism may be related to the improvement on liver peroxidation injury in rats.