Study onquality control of Dongbo Tonglin Granule

ZHU Jianyong; ZHANG Chunyan; LI Xiaoqin; ZHANG Lijun; ZHANG Hong

doi:10.16305/j.1007-1334.2018.08.024

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Study onquality control of Dongbo Tonglin Granule

更新时间：2022-08-31

- Study onquality control of Dongbo Tonglin Granule
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 8, Pages: 87-92(2018)
- 作者机构：
  
  1. 上海中医药大学附属第七人民医院中心实验室,上海,200137
  2. 成都中医药大学药学院中药学教研室,四川,成都,611137
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.08.024
  CLC：
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ZHU Jianyong, ZHANG Chunyan, LI Xiaoqin, et al. Study onquality control of Dongbo Tonglin Granule. [J]. Shanghai Journal of Traditional Chinese Medicine 52(8):87-92(2018)
DOI：

ZHU Jianyong, ZHANG Chunyan, LI Xiaoqin, et al. Study onquality control of Dongbo Tonglin Granule. [J]. Shanghai Journal of Traditional Chinese Medicine 52(8):87-92(2018) DOI： 10.16305/j.1007-1334.2018.08.024.

摘要

目的：建立冬柏通淋颗粒(黄柏、忍冬藤、萹蓄)的质量控制方法。方法：采用薄层色谱法对该复方颗粒中黄柏、忍冬藤、萹蓄进行定性鉴别,同时采用高效液相色谱法测定小檗碱和绿原酸两种主成分的含量。结果：TLC 鉴别分离度良好,专属性强,阴性样品无干扰；小檗碱对照品在0.108~2.16 μg (r=0.999 5) 范围内线性关系良好,加样回收率均值为101.61%,RSD为2.53%；绿原酸对照品在0.114~2.28 μg (r=0.999 6) 范围内线性关系良好,加样回收率均值为99.91%,RSD为2.32%。结论：该方法操作简便易行,专属性强,准确可靠,可用于冬柏通淋颗粒的质量控制。

Abstract

Objective:To establish the quality control method for Dongbo Tonglin Granule, which includes Huangbo (Phellodendri Chinensis Cortex), Rendongteng (Lonicerae Japonicae Caulis) and Bianxu (Polygoni Avicularis Herba). MethodsThe identifications of Huangbo, Rendongteng and Bianxu in this compound prescription were conducted by thin layer chromatography (TLC). The contents of berberine and chlorogenic acid were determined by high performance liquid chromatography (HPLC). Results:The identification and separation of TLC was good and highly specific, and no interference was observed in the negative sample. The berberine showed a good linearity in the range of 0.108~2.16 μg (r=0.999 5). The average recovery was 101.61%(RSD=2.53%). The chlorogenic acid showed a good linearity in the range of 0.114~2.28 μg (r=0.999 6). The average recovery was 99.91%(RSD=2.32%). Conclusion:This method is simple, accurate and reliable with strong specificity, and can be used for the quality control of Dongbo Tonglin Granule.

关键词

冬柏通淋颗粒薄层色谱高效液相色谱小檗碱绿原酸

Keywords

Dongbo Tonglin Granulethin layer chromatographyhigh performance liquid chromatographyberberinechlorogenic acid

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