Objective:To explore the effects of (-)-Gallocatechin on the glucose uptake when the adipocytes were treated with high concentration of glucose. MethodsThe effects of (-)-Gallocatechin on glucose uptake and differentiation of 3T3-L1 preadipocytes were detected using a 3T3-L1 preadipocytes differentiation model.  Results:The (-)-Gallocatechin increased insulin-stimulated glucose uptake in adipocytes under the condition of high-concentration glucose(30 mmol/L) and accelerated the differentiation of 3T3-L1 preadipocyte.  Conclusion:The (-)-Gallocatechin increases the glucose uptake in adipocytes and promotes the differentiation of 3T3-L1 preadipocytes,which suggests that (-)-Gallocatechin may effectively improve the glycemic control and ameliorate the insulin resistance.