Inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice

WU Ruiying; ZHANG Yong; LIANG Fang; CHEN Bin; WAN Guangsheng; LIANG Mengting; XU Jianhua

doi:10.16305/j.1007-1334.2018.06.020

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Inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice

更新时间：2022-08-31

- Inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 6, Pages: 62-66(2018)
- 作者机构：
  
  1. 上海中医药大学附属普陀医院肿瘤科,上海,200333
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.06.020
  CLC：
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WU Ruiying, ZHANG Yong, LIANG Fang, et al. Inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice. [J]. Shanghai Journal of Traditional Chinese Medicine 52(6):62-66(2018)
DOI：

WU Ruiying, ZHANG Yong, LIANG Fang, et al. Inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice. [J]. Shanghai Journal of Traditional Chinese Medicine 52(6):62-66(2018) DOI： 10.16305/j.1007-1334.2018.06.020.

摘要

目的：研究肠胃清对化疗诱导的小鼠结肠癌肺转移的抑制作用并探讨其机制。方法：通过一次性腹腔注射最大耐受剂量(MTD)奥沙利铂,3天后尾静脉注射结肠癌细胞CT26,建立化疗诱导的小鼠结肠癌肺转移模型,24只BALB/c小鼠随机分为模型组、化疗诱导肺转移组(L-OHP)、肠胃清加化疗诱导肺转移组(CWQ+L-OHP)。统计肺结节数目,苏木素-伊红染色(HE)检测肺组织内瘤灶,免疫组化检测肺转移瘤组织微血管密度(MVD)；蛋白免疫印迹(Western blot)、实时荧光定量PCR(qRT-PCR)检测肺转移灶血管内皮生长因子(VEGF-A)、基质金属蛋白酶2/9(MMP2/9)蛋白和mRNA的表达。结果：与L-OHP组比较,CWQ+L-OHP组肺转移结节数目减少(P<0.05)、肿瘤组织MVD值下降(P<0.01),肺组织VEGF-A、MMP2、MMP9蛋白和mRNA的表达均下调(P<0.01)。结论：肠胃清能抑制化疗诱导的小鼠结肠癌肺转移,与其下调肿瘤组织VEGF-A、MMP2、MMP9的表达,降低肿瘤组织MVD,减少肿瘤血管生成有关。

Abstract

Objective:To study the inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice and explore its mechanism. MethodsThe chemotherapy induced lung metastasis of colon cancer model was established by one-time intraperitoneal injection of oxaliplatin(L-OHP)at maximum tolerable dose and intravenous injection of colon cancer CT26 cells via caudal vein 3 days later. 24 BALB/c mice were randomly divided into the model group,chemotherapy induced lung metastasis(L-OHP)group,Changweiqing plus chemotherapy induced lung metastasis(CWQ+L-OHP)group. The nodules number of lung metastasis was counted,the tumor lesions in the lung were detected by HE staining,the microvessel density(MVD)of lung metastatic tumor tissue was detected by immunohistochemistry,and the protein and mRNA expressions of vascular endothelial growth factor(VEGF)-A,matrix metalloproteinase 2/9(MMP2/9)in the lung metastasis were detected by Western blot and qRT-PCR. Results:Compared with the L-OHP group,the nodules number of lung metastasis was reduced in the CWQ+L-OHP group(P<0.05),and the MVD value of tumor tissue was decreased(P<0.01),and the protein and mRNA expressions of VEGF-A,MMP2 and MMP9 in the lung tissue were all down-regulated(P<0.01). Conclusion:Changweiqing can inhibit chemotherapy induced lung metastasis of colon cancer in mice. Its mechanism may be related to down-regulating the expressions of VEGF-A,MMP2 and MMP9 in tumor tissue and reducing MVD and tumor angiogenesis.

关键词

肠胃清化疗结肠癌肺转移血管内皮生长因子基质金属蛋白酶

Keywords

Changweiqingchemotherapycolon cancerlung metastasisvascular endothelial growth factormatrix metalloproteinase

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