Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34<sup>+</sup> cell apoptosis in normal bone marrow based on P38MAPK pathway

ZHU Xiaoqin; ZHAO Lin; QIU Zhongchuan; CHEN Pei; HUANG Zhongdi; HU Xiaoying; QU Weiying; LU Yingjia; FENG Zhou

doi:10.16305/j.1007-1334.2018.04.026

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Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34⁺ cell apoptosis in normal bone marrow based on P38MAPK pathway

更新时间：2022-08-31

- Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34⁺ cell apoptosis in normal bone marrow based on P38MAPK pathway
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 4, Pages: 96-100(2018)
- 作者机构：
  
  1. 上海中医药大学附属曙光医院血液科,上海,200021
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.04.026
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ZHU Xiaoqin, ZHAO Lin, QIU Zhongchuan, et al. Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34⁺ cell apoptosis in normal bone marrow based on P38MAPK pathway. [J]. Shanghai Journal of Traditional Chinese Medicine 52(4):96-100(2018)
DOI：

ZHU Xiaoqin, ZHAO Lin, QIU Zhongchuan, et al. Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34⁺ cell apoptosis in normal bone marrow based on P38MAPK pathway. [J]. Shanghai Journal of Traditional Chinese Medicine 52(4):96-100(2018) DOI： 10.16305/j.1007-1334.2018.04.026.

摘要

目的：基于P38MAPK途径探讨复方参鹿颗粒含药血清对TNF-α诱导的正常骨髓CD34,+,细胞凋亡的影响。方法：Wistar大鼠随机分为空白组和复方参鹿颗粒低、中、高剂量组,分别制备空白血清和不同浓度的含药血清。收集并分离8例正常的骨髓单个核细胞(BMMC),分别加入空白血清和10％不同浓度含药血清,与20 ng/ml人重组TNF-α共同培养。流式细胞术检测CD34,+,细胞凋亡率,Western blot检测骨髓单个核细胞P38、p-P38、P53、p-P53、Bcl-xl、Bcl-2蛋白表达,荧光定量PCR检测P38、P53、Bcl-xl、Bcl-2 mRNA表达。结果：与空白组相比,复方参鹿颗粒中、高剂量组CD34,+,细胞凋亡率以及p-P38、p-P53蛋白表达显著下降,Bcl-xl蛋白和mRNA表达显著升高,差异有统计学意义(P＜0.05)。结论：复方参鹿颗粒含药血清可有效抑制TNF-α诱导的正常骨髓CD34,+,细胞凋亡,下调p-P38和p-P53蛋白表达,上调Bcl-xl表达。其机制可能与P38MAPK通路介导的凋亡途径有关。

Abstract

Objective:To discuss the effects of medicated serum of Compound Shenlu Granule on TNF- α induced CD34,+, cell apoptosis in normal bone marrow based on P38MAPK pathway. MethodsWistar rats were randomly divided into the blank group and Compound Shenlu Granule groups with low-,medium- and high-dose. The blank serum and medicated serum at different concentrations were prepared respectively. The normal bone marrow mononuclear cells (BMMCs) from 8 individuals were collected and isolated. The BMMCs were treated with blank serum or 10% medicated serum at different concentrations,which co-cultured with 20 ng/ml TNF- α. The apoptosis rate of CD34,+,cells was detected by flow cytometry. The protein expressions of P38,p-P38,P53,p-P53,Bcl-xl and Bcl-2 in BMMCs were detected by Western blot. The mRNA expressions of P38,P53,Bcl-xl and Bcl-2 were detected by fluorescence quantitative PCR. Results:Compared with the blank group,the apoptosis rate of CD34,+, cells and the protein expressions of p-P38 and p-P53 were significantly decreased in the Compound Shenlu Granule groups with medium- and high-dose,and the Bcl-xl protein and mRNA expressions were significantly up-regulated,with statistically significant differences (P,<,0.05). Conclusion:The medicated serum of Compound Shenlu Granule can effectively inhibit the TNF- α induced CD34,+, cell apoptosis in normal bone marrow,down-regulate the protein expressions of p-P38 and p-P53 and up-regulate the expression of Bcl-xl. Its mechanism may be related to the P38MAPK-mediated apoptosis pathway.

关键词

复方参鹿颗粒含药血清P38MAPKCD34+

Keywords

Compound Shenlu Granulemedicated serumP38MAPKCD34+

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Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34+ cell apoptosis in normal bone marrow based on P38MAPK pathway

DOI：10.16305/j.1007-1334.2018.04.026

摘要

Abstract

关键词

Keywords

references

Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34⁺ cell apoptosis in normal bone marrow based on P38MAPK pathway