Objective:To observe the autophagy induced by cisplatin in A549 lung cancer cells and the effects of Feiyanning decoction on the autophagy related proteins including autophagy related gene 5 (Atg5) and autophagy related gene 7 (Atg7). MethodsThe A549 lung cancer cells were cultured and treated with cisplatin and Feiyanning decoction. The optimized concentrations of cisplatin and Feiyanning were screened by cell counting kit-8 (CCK-8) assay. The proliferation ability of A549 cells in each group was measured by CCK-8 assay. The expressions of autophagy related proteins in each group were detected by Western blot.  Results:Compared with cisplatin alone,3 mg/L cisplatin combined with 20 mmol/L chloroquine(autophagy inhibitor)significantly increased the inhibition rate of A549 cell proliferation (P<0.05). Meanwhile,the protein expression of microtubule-associated protein 1 light chain 3(LC3Ⅱ)was increased after cisplatin treatment (P<0.01). The Atg5 protein expression in the Feiyanning group was lower than that in the model group and the cisplatin group (P<0.05),and the Atg7 protein expression in the Feiyanning group was significantly lower than that in the model group and the cisplatin group (P<0.01).  Conclusion:Cisplatin induces protective autophagy in A549 lung cancer cells,and Feiyangning may suppress the cell proliferation by inhibiting autophagy in lung cancer cells.