Inhibition effects of water extract of Weiqi Decoction on growth and tube formation in human umbilical vein endothelial cells

YI Jinyu; YIN Jing; XU Bo; CHEN Ye; LI Hongli; QIU Shuiping; WU Xiaojun; GONG Yuping; LIN Jiang; WANG Zhengtao; SHI Hailian; FEI Xiaoyan

doi:10.16305/j.1007-1334.2018.04.021

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Inhibition effects of water extract of Weiqi Decoction on growth and tube formation in human umbilical vein endothelial cells

更新时间：2022-08-31

- Inhibition effects of water extract of Weiqi Decoction on growth and tube formation in human umbilical vein endothelial cells
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 4, Pages: 73-76(2018)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院脾胃病科,上海,200032
  2. 上海中医药大学附属龙华医院消化内镜科,上海,200032
  3. 上海中医药大学中药所,上海,201203
  4. 上海中医药大学复方中药重点实验室,上海,201203
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.04.021
  CLC：
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YI Jinyu, YIN Jing, XU Bo, et al. Inhibition effects of water extract of Weiqi Decoction on growth and tube formation in human umbilical vein endothelial cells. [J]. Shanghai Journal of Traditional Chinese Medicine 52(4):73-76(2018)
DOI：

YI Jinyu, YIN Jing, XU Bo, et al. Inhibition effects of water extract of Weiqi Decoction on growth and tube formation in human umbilical vein endothelial cells. [J]. Shanghai Journal of Traditional Chinese Medicine 52(4):73-76(2018) DOI： 10.16305/j.1007-1334.2018.04.021.

摘要

目的：探讨胃祺饮水提液对人脐静脉内皮细胞(HUVECs)生长和管腔形成能力的影响及其分子机制。方法：取人脐静脉内皮细胞(HUVECs)分为6组,分别采用0、0.125、0.250、0.500、1 mg·ml-1的胃祺饮水提物和30 μmol/L的5-FU进行培养。培养24 h,采用CCK-8检测不同浓度的胃祺饮方对HUVECs细胞增殖的影响；Matrigel实验检测HUVECs管腔形成能力；Western blot检测MAPK通路相关蛋白(ERK、p38)及其磷酸化水平的蛋白表达。结果：胃祺饮水提液能够抑制HUVECs细胞的存活率,并能够剂量依赖性抑制HUVECs细胞管腔形成能力(P<0.01),且胃祺饮水提液能够显著下调HUVECs细胞MAPK通路相关蛋白(ERK、p38)及其磷酸化水平(P<0.01)。结论：胃祺饮水提液可抑制HUVECs细胞的存活率和管腔形成能力,其机制可能与下调MAPK通路相关蛋白(ERK、p38)的磷酸化水平有关。

Abstract

Objective:To investigate the effects and molecular mechanisms of water extract of Weiqi Decoction on the growth and tube formation of human umbilical vein endothelial cells (HUVECs). MethodsThe HUVECs were divided into 6 groups and treated with water extract of Weiqi Decoction at concentrations of 0,0.125,0.25,0.5 and1 mg·ml-1 or 5-FU at concentration of 30 μmol/L,respectively. After 24 hours culture,the effects of Weiqi Decoction at different concentrations on the cell proliferation of HUVECs were detected by CCK-8 assay. The tube formation of HUVECs was detected by Matrigel assay. The protein expressions and phosphorylation levels of MAPK pathway-related proteins (ERK and p38) were detected by Western blot. Results:The water extract of Weiqi Decoction could inhibit the survival rate of HUVECs. In addition,the water extract of Weiqi Decoction could inhibit the tube formation of HUVECs in a dose-dependent manner (P<0.01),and significantly down-regulate the protein expressions and phosphorylation levels of MAPK pathway-related proteins (ERK and p38) in HUVECs (P<0.01). Conclusion:The water extract of Weiqi Decoction can inhibit the survival rate and tube formation of HUVECs. The mechanism may be associated with the down-regulation on the expressions and phosphorylation levels of MAPK pathway related proteins (ERK and p38).

关键词

胃祺饮人脐静脉内皮细胞血管新生细胞外信号调节激酶

Keywords

Weiqi Decoctionhuman umbilical vein endothelial cellsangiogenesisextracellular signal-regulated kinase

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