Regulation of Gutongling Decoction on MAPK signaling pathway in spinal cord of bone metastasis mice

WANG Lifang; JIN Guiyu; LUO Qinqin; LYU Ying; MA Yue; XU Zhenye

doi:10.16305/j.1007-1334.2018.03.018

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Regulation of Gutongling Decoction on MAPK signaling pathway in spinal cord of bone metastasis mice

更新时间：2022-08-31

- Regulation of Gutongling Decoction on MAPK signaling pathway in spinal cord of bone metastasis mice
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 3, Pages: 68-71(2018)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院肿瘤科,上海,200032
  2. 上海中医药大学附属岳阳中西医结合医院肿瘤科,上海,200437
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.03.018
  CLC：
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WANG Lifang, JIN Guiyu, LUO Qinqin, et al. Regulation of Gutongling Decoction on MAPK signaling pathway in spinal cord of bone metastasis mice. [J]. Shanghai Journal of Traditional Chinese Medicine 52(3):68-71(2018)
DOI：

WANG Lifang, JIN Guiyu, LUO Qinqin, et al. Regulation of Gutongling Decoction on MAPK signaling pathway in spinal cord of bone metastasis mice. [J]. Shanghai Journal of Traditional Chinese Medicine 52(3):68-71(2018) DOI： 10.16305/j.1007-1334.2018.03.018.

摘要

目的：从调控MAPK信号通路角度探讨骨痛灵方治疗骨转移疼痛的作用机制。方法：将40只小鼠随机分为假手术组、模型组、中药组、对照组,除假手术组外,其余各组建立小鼠骨转移模型。从造模第7天开始给药,中药组予骨痛灵方治疗,对照组以曲马多治疗,采用VonFrey纤维检测机械痛阈值评价其止痛疗效,观察肝肾心病理评价其安全性,采用Western blot法检测脊髓MAPK信号通路中p-JNK、p-p38、p-ERK蛋白的表达。结果：①造模第14天,与模型组比较,对照组和中药组机械痛阈值均有升高(P<0.05)。造模第21天,中药组机械痛阈值较模型组与对照组均增高(P<0.05)。②各组心肝肾病理均无明显改变。③与假手术组比较,模型组脊髓p-JNK、p-p38、p-ERK的表达均增加(P<0.05)。与模型组比较,对照组和中药组脊髓p-JNK、p-p38和p-ERK的表达均显著减少(P<0.05)。中药组和对照组脊髓p-JNK、p-p38和p-ERK的表达差异均无统计学意义(P>0.05)。结论：骨痛灵具有良好的治疗骨转移疼痛的疗效,且对心肝肾无明显毒副作用,其止痛作用机制与调控MAPK信号通路p-JNK、p-p38、p-ERK蛋白表达有关。

Abstract

Objective:To discuss the mechanism of Gutongling Decoction in the treatment of pain due to bone metastasis based on the regulation of MAPK signaling pathway. MethodsThe mouse model of bone metastasis was established,and 40 mice were randomly divided into the sham operation group, model group, Chinese medicine group and control group. Except the sham operation group, the mouse model of bone metastasis was established in the other groups. From 7th day of modeling, the Chinese medicine group was treated with Gutongling Decoction and the control group was treated with tramadol. The mechanical pain threshold was detected by VonFrey fiber to evaluate the analgesic efficacy. The pathology of liver, kidney and heart were observed to evaluate the drug safety. The protein expressions of p-JNK, p-p38 and p-ERK in MAPK signaling pathway in spinal cord were detected by Western blot. Results:①On the 14th day after modeling, compared with the model group, the mechanical pain threshold was increased in both control group and Chinese medicine group (P<0.05). On the 21st day after modeling, compared with the model group and control group, the mechanical pain threshold was increased in the Chinese medicine group (P<0.05). ②There were no significant changes on the pathology of liver, kidney and heart. ③Compared with the sham operation group, the expressions of p-JNK, p-p38 and p-ERK in spinal cord were increased in the model group (P<0.05). Compared with the model group, the expressions of p-JNK, p-p38 and p-ERK were significantly decreased in the control group and Chinese medicine group (P<0.05). There were no significant differences on the expressions of p-JNK, p-p38 and p-ERK between the Chinese medicine group and control group (P>0.05). Conclusion:Gutongling Decoction shows good efficacy in the treatment of pain due to bone metastasis, with no obvious adverse effects on heart, liver and kidney. Its analgesic mechanism is related to regulating the protein expressions of p-JNK, p-p38, p-ERK in MAPK signaling pathway.

关键词

骨转移疼痛骨痛灵方MARK信号通路

Keywords

bone metastasispainGutongling DecoctionMARK signaling pathway

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