Effects of icariin on phenotype and metabolism in IL-1β-induced inflammatory chondrocytes

LI Anqi; LIU Yijie; FENG Wei; HE Dongyi; ZHANG Zengqiao; HU Xiaoshen; YANG Songbin; ZHANG Cai

doi:10.16305/j.1007-1334.2018.02.024

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Effects of icariin on phenotype and metabolism in IL-1β-induced inflammatory chondrocytes

更新时间：2022-08-31

- Effects of icariin on phenotype and metabolism in IL-1β-induced inflammatory chondrocytes
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 2, Pages: 99-103(2018)
- 作者机构：
  
  1. 上海中医药大学康复医学院,上海,201203
  2. 上海市光华中西医结合医院,上海,200052
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.02.024
  CLC：
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LI Anqi, LIU Yijie, FENG Wei, et al. Effects of icariin on phenotype and metabolism in IL-1β-induced inflammatory chondrocytes. [J]. Shanghai Journal of Traditional Chinese Medicine 52(2):99-103(2018)
DOI：

LI Anqi, LIU Yijie, FENG Wei, et al. Effects of icariin on phenotype and metabolism in IL-1β-induced inflammatory chondrocytes. [J]. Shanghai Journal of Traditional Chinese Medicine 52(2):99-103(2018) DOI： 10.16305/j.1007-1334.2018.02.024.

摘要

目的：探讨淫羊藿苷(Icariin,ICA)对IL-1β诱导的炎症软骨细胞表型的影响。方法：分离培养大鼠关节软骨细胞,利用白细胞介素-1β(interleukin-1β,IL-1β)诱导原代软骨细胞,模拟骨关节炎软骨细胞炎症内坏境。实验分为空白组、模型组(含IL-1β 10 mg/L)、ICA低浓度组(含10 mg/L IL-1β和1 μmol/L ICA)和ICA 高浓度组(含10 mg/L IL-1β和5 μmol/L ICA)。利用Western blot的方法检测II=2\*ROMAN型胶原蛋白(collagen-II=2\*ROMAN,Col-II)和蛋白聚糖(aggrecan)蛋白表达情况,利用RT-PCR法检测collagen-II=2\*ROMAN、aggrecan、MMP-13、ADAMTS-5 mRNA表达情况。结果：培养3天时,与模型组比较,ICA对炎症软骨细胞collagen-II mRNA和蛋白表达有明显的促进作用(P<0.05),ICA高浓度组对collagen-II mRNA和蛋白表达的促进作用较低浓度组更强(P<0.05)；与模型组比较,ICA对炎症软骨细胞aggrecan mRNA和蛋白表达有明显的促进作用(P<0.05),不同浓度的ICA促进作用差异不明显。与模型组比较,ICA可抑制MMP-13、ADAMTS-5 mRNA表达(P<0.05),ICA高浓度组抑制MMP-13 mRNA表达作用更显著(P<0.05),不同浓度ICA对ADAMTS-5 mRNA表达差异不明显。结论：ICA可以通过抑制MMP-13、ADAMTS-5的表达,促进炎症软骨细胞collagen-II=2\*ROMAN和aggrecan的表达,从而维持软骨细胞表型和促进基质代谢。

Abstract

Objective:To investigate the effects of icariin (ICA) on the phenotype of interleukin-1β (IL-1β)-induced inflammatory chondrocytes. MethodsThe articular chondrocytes of rats were isolated and cultured. The primary chondrocytes were induced by IL-1β,and the inflammatory environment of chondrocytes in osteoarthritis was simulated. The cells were divided into the blank group,model group(treated with IL-1β at concentration of 10 mg/L),ICA group with low-concentration(treated with IL-1β at concentration of 10 mg/L and ICA at concentration of 1 μmol/L)and ICA group with high-concentration(treated with IL-1β at concentration of 10 mg/L and ICA at concentration of 5 μmol/L).The protein expressions of collagen-II and aggrecan were detected by Western blot. The mRNA expressions of collagen-II,aggrecan,matrix metallopeptidase-13(MMP-13)and a disintegrin and metalloprotease with thrombospondin motifs-5(ADAMTS-5)were detected by RT-PCR. Results:After the cells were cultured for three day,compared with the model group,ICA could obviously promote the mRNA and protein expressions of collagen-II in the inflammatory chondrocytes(P<0.05)；and this effect in the ICA group with high-concentration was more significant than that in the ICA group with low-concentration(P<0.05). Compared with the model group,ICA could obviously promote the mRNA and protein expressions of aggrecan in the inflammatory chondrocytes(P<0.05)；and this effect shows no obviously difference between the low- and high-concentration. Compared with the model group,ICA could inhibit the mRNA expressions of MMP-13 and ADAMTS-5(P<0.05),and the inhibiting effect on MMP-13 mRNA expression was more significant in the ICA group with high-concentration(P<0.05)；there was no obvious difference on the ADAMTS-5 mRNA expression between the low- and high-concentration. Conclusion:ICA can inhibit the expressions of MMP-13 and ADAMTS-5 and promote the expressions of collagen-II and aggrecan in the inflammatory chondrocytes,and thus maintain the chondrocytes phenotype and promote the matrix metabolism.

关键词

淫羊藿苷骨关节炎软骨细胞

Keywords

icariinOsteoarthritischondrocytes

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