Effects of Changweiqing Decoction on DNA double-strand damage and homologous recombination repair factor ATM and γH2AX in nude mice with human colon cancer subcutaneous xenograft

ZHANG Ruijuan; YU Qianyun; LI Chaoheng; JIANG Wei; XU Jianhua

doi:10.16305/j.1007-1334.2018.02.022

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Effects of Changweiqing Decoction on DNA double-strand damage and homologous recombination repair factor ATM and γH2AX in nude mice with human colon cancer subcutaneous xenograft

更新时间：2022-08-31

- Effects of Changweiqing Decoction on DNA double-strand damage and homologous recombination repair factor ATM and γH2AX in nude mice with human colon cancer subcutaneous xenograft
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 2, Pages: 89-94(2018)
- 作者机构：
  
  1. 上海市普陀区人民医院中医科,上海,200060
  2. 上海市黄浦区五里桥街道社区卫生服务中心中医科,上海,200023
  3. 上海中医药大学附属普陀医院中医肿瘤科,上海,200062
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.02.022
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ZHANG Ruijuan, YU Qianyun, LI Chaoheng, et al. Effects of Changweiqing Decoction on DNA double-strand damage and homologous recombination repair factor ATM and γH2AX in nude mice with human colon cancer subcutaneous xenograft. [J]. Shanghai Journal of Traditional Chinese Medicine 52(2):89-94(2018)
DOI：

ZHANG Ruijuan, YU Qianyun, LI Chaoheng, et al. Effects of Changweiqing Decoction on DNA double-strand damage and homologous recombination repair factor ATM and γH2AX in nude mice with human colon cancer subcutaneous xenograft. [J]. Shanghai Journal of Traditional Chinese Medicine 52(2):89-94(2018) DOI： 10.16305/j.1007-1334.2018.02.022.

摘要

目的：研究肠胃清对裸鼠结肠癌皮下移植瘤DNA双链损伤及同源重组修复因子ATM、γH2AX的影响。方法：构建裸鼠人结肠癌细胞HCT116皮下移植瘤模型,分为空白组、L-OHP组、肠胃清组、肠胃清联合L-OHP组(联合组),分别采用NaCl溶液、L-OHP、肠胃清、肠胃清联合L-OHP干预。运用彗星扫尾实验检测DNA损伤程度,Western blot及免疫组化法检测同源重组修复因子ATM、γH2AX的表达。结果：与空白组比较,肠胃清组、L-OHP组和联合组均出现细胞拖尾现象,其中联合组细胞拖尾现象最明显(P<0.05)。与空白组比较,L-OHP组和联合组DNA同源重组修复因子ATM、γH2AX 蛋白表达明显上调(P<0.05)；与L-OHP组比较,联合组ATM、γH2AX蛋白表达明显下调(P<0.05 ,P<0.01)。结论：肠胃清联合L-OHP 组中DNA双链断裂损伤最严重,提示肠胃清可增强L-OHP的疗效,其作用机制是减少结肠癌细胞对化疗损伤的同源重组修复作用。

Abstract

Objective:To study the effects of Changweiqing Decoction on DNA double-strand damage and homologous recombination repair factor ATM and γH2AX in nude mice with subcutaneous xenografts of colon cancer . MethodsThe subcutaneous xenografts model was established in the nude mice by human colon cancer cell line(HCT116). The nude mice were divided into the blank group,oxaliplatin(L-OHP) group,Changweiqing group and Changweiqing plus L-OHP group(combination group). The drug treatment groups were treated with L-OHP,Changweiqing Decoction and Changweiqing Decoction plus L-OHP,respectively. The degree of DNA damage was detected by comet assay. The expressions of homologous recombination factor ATM and γH2AX were detected by Western blot and immunohistochemistry. Results:Compared with the blank group,the tailing phenomenon was observed in the Changweiqing group,L-OHP group and combination group,and the tailing phenomenon was more significant in the combination group(P<0.05). Compared with the blank group,the protein expressions of ATM and γH2AX were obviously up-regulated in the L-OHP group and combination group(P<0.05). Compared with the L-OHP group,the protein expressions of ATM and γH2AX were obviously down-regulated in the combination group(P<0.05,P<0.01). Conclusion:The most serious damage of DNA double-strand breaks can be observed in the Changweiqing plus L-OHP group,which suggests that Changweiqing Decoction can enhance the efficacy of L-OHP. Its mechanism is to reduce the homologous recombination repair effects of colon cancer cells on the chemotherapy damage.

关键词

结肠癌肠胃清奥沙利铂DNA双链损伤同源重组修复

Keywords

colon cancerChangweiqingoxaliplatinDNA double-strand damagehomologous recombination repair

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