6-Shogao induces apoptosis of BGC-823 cells and its mechanism

ZHAO Xingyu; HOU Yisen; LIU Yafan; HOU Jiancheng; ZHANG Xiaoyu; ZHANG Wei

doi:10.16305/j.1007-1334.2018.02.021

您当前的位置：

首页 >

文章列表页 >

6-Shogao induces apoptosis of BGC-823 cells and its mechanism

更新时间：2022-08-31

- 6-Shogao induces apoptosis of BGC-823 cells and its mechanism
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 2, Pages: 84-88(2018)
- 作者机构：
  
  1. 吉林医药学院生物化学教研室,吉林,吉林,132013
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2018.02.021
  CLC：
扫描看全文
ZHAO Xingyu, HOU Yisen, LIU Yafan, et al. 6-Shogao induces apoptosis of BGC-823 cells and its mechanism. [J]. Shanghai Journal of Traditional Chinese Medicine 52(2):84-88(2018)
DOI：

ZHAO Xingyu, HOU Yisen, LIU Yafan, et al. 6-Shogao induces apoptosis of BGC-823 cells and its mechanism. [J]. Shanghai Journal of Traditional Chinese Medicine 52(2):84-88(2018) DOI： 10.16305/j.1007-1334.2018.02.021.

摘要

目的：研究不同浓度6-姜烯酚对胃癌BGC-823细胞凋亡的影响,并探讨其作用机制。方法：不同浓度的6-姜烯酚(0、10、20、50 μmol/L)分别作用于人胃癌BGC-823细胞24 h后,倒置显微镜观察细胞形态变化,MTT法检测各组细胞存活情况,流式细胞仪检测细胞早期凋亡率,Western blot检测凋亡相关蛋白P53、剪切型聚腺苷二磷酸核糖聚合酶-1(cleaved-poly ADP-ribose polymerase-1,cleaved-PARP-1)、非活性天冬氨酸特异性半胱氨酸蛋白酶-3(pro-cysteinyl aspartate specific proteinase-3,pro-Caspase-3)、剪切型Caspase-3(cleaved-Caspase-3)表达的变化。结果：6-姜烯酚对BGC-823细胞生长有明显的抑制作用。与对照组相比,6-姜烯酚各浓度组细胞的存活抑制率和早期凋亡率显著性升高(P<0.05,P<0.01),且具有一定的浓度依赖性。Western blot检测显示,6-姜烯酚可促进BGC-823细胞P53、cleaved-Caspase-3、cleaved-PARP-1蛋白的表达增加,而降低pro-Caspase-3蛋白的表达(P<0.05,P<0.01)。结论：6-姜烯酚可诱导胃癌BGC-823细胞凋亡,其机制可能与Caspase-3介导的细胞凋亡途径有关。

Abstract

Objective:To study the effects of 6-Shogao at different concentrations on the apoptosis of human gastric cancer BGC-823 cells,and discuss its mechanism. MethodsThe BGC-823 cells were treated with 6-Shogao at concentrations of 0,10,20 and 50 μmol/L for 24 hours,respectively. The morphological changes of cells were observed by inverted microscope. The viability of BGC-823 cells in each group was detected by MTT assay. The early apoptosis rate was detected by flow cytometry. The changes on the expression of apoptosis related proteins including P53,cleaved-poly ADP-ribose polymerase-1(cleaved-PARP-1),pro-cysteinyl aspartate specific proteinase-3(pro-Caspase-3)and cleaved-Caspase-3 were detected by Western blot. Results:6-Shogao showed the significant inhibitory effect on the growth of BGC-823 cells. Compared with the control group,the inhibition rate and early apoptosis rate of cells were increased significantly in the 6-Shogao-treated groups with different concentrations(P<0.05,P<0.01),in a concentration-dependent manner. The results of Western blot showed that 6-Shogao could increase the protein expressions of P53,cleaved-Caspase-3 and cleaved-PARP-1 and decrease the pro-Caspase-3 protein expression in BGC-823 cells(P<0.05,P<0.01). Conclusion:6-Shogao can induce BGC-823 cells apoptosis. Its mechanism may be associated with Caspase-3 mediated apoptosis pathway.

关键词

6-姜烯酚BGC-823细胞细胞增殖细胞凋亡caspase-3

Keywords

6-ShogaoBGC-823 cellscell proliferationcell apoptosiscaspase-3

references

Views

196

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on proliferation and migration effects of Yiqifusheng prescription on human gastric cancer cell line BGC-823

Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer

Research progress on relationship between skeletal muscle atrophy and apoptosis in COPD based on theory of “spleen dominating muscles” and intervention effect of traditional Chinese medicine

Study on anti⁃apoptotic mechanism of Tuina combined with spinal cord electrical stimulation in anterior horn motor neurons of spinal cord in rats with sciatic nerve injury

Research progress on effect of oxidative stress in thyroid cancer and intervention of Chinese medicine monomer in vitro

Related Author

No data

Related Institution

Fu Yan-Li Chinese Medicine Clinic,Uruba City,Xianjiang Uygur Autonomous Region

Department of Oncology,The Fourth Affiliated Hospital of Xinjiang Medical University

Tumor Institute of Traditional Chinese Medicine, Shanghai Academy of Traditional Chinese Medicine

Tumor Institute of Traditional Chinese Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Pneumology Department， Yueyang Hospital of Integrated Traditional Chinese and Western Medicine， Shanghai University of Traditional Chinese Medicine

⁰