Objective:To study the material basis of model mice with deficiency syndrome of qi,blood,yin and yang in viscera state of kidney. MethodsTaken the male ICR mice as objects,the model of syndrome of qi deficiency was replicated by dietary restriction method,the model of syndrome of blood deficiency was replicated by acetylphenylhydrazine,the model of syndrome of yin deficiency was replicated by thyroxine,the model of syndrome of yang deficiency was replicated by hydrocortisone. The body weight of mice was observed everyday,and the changes on different organs were compared. RNA was extracted in the kidney,adrenal and bone marrow tissue. The related gene expressions were detected by real time fluorescence quantitative PCR. The serum content of corticosterone was detected by ELISA.  Results:①Compared with the normal control group,the reduction on body weight of mice was most significant in the dietary restriction group(P<0.01),the body weight of mice in the acetylphenylhydrazine group and hydrocortisone group were also significantly decreased(P<0.05).The obvious atrophy in the spleen and thymus was observed in the dietary restriction group and hydrocortisone group(P<0.01),the significant swelling in the spleen and severe atrophy in the thymus were found in the acetylphenylhydrazine group(P<0.01). ②Compared with the normal control group,the serum level of corticosterone was significantly increased in the dietary restriction group and acetylphenylhydrazine group(P<0.05),while the serum level of corticosterone was significantly decreased in the hydrocortisone group(P<0.001). ③In the dietary restriction group,the mRNA expressions of Star,Cyp21a1 and Cyp11b1 in the adrenal gland were significantly increased(P<0.05),and the Epo mRNA expression in the kidney was significantly increased(P<0.05)and the Csf2 mRNA expression in the kidney significantly decreased(P<0.05),the mRNA expressions of Tpo and IL-4 in the bone marrow were significantly up-regulated(P<0.05). ④In the acetylphenylhydrazine group,the mRNA expressions of Cyp11a1,Cyp21a1 and Cyp11b1 in the adrenal gland were significantly increased(P<0.05),the mRNA expressions of Epo,Csf2 and Csf3 in the kidney were significantly increased(P<0.05),the mRNA expressions of Csf3,IL-4,IL-5 and IL-6 in the bone marrow were significantly increased,but the mRNA expressions of IL-1b and IL-7 in the bone marrow were significantly decreased(P<0.05). ⑤In the thyroxine group,the Cyp11b1 mRNA expression in the adrenal gland was significantly increased(P<0.05),the Epo mRNA expression was significantly increased and Csf2 mRNA expression was significantly decreased in the kidney(P<0.05),the mRNA expressions of Csf3,IL-2 and IL-5 in the bone marrow were significantly decreased(P<0.05). ⑥In the hydrocortisone group,the mRNA expressions of Star,Cyp21a1,Cyp11b1 and Cyp11b2 in the adrenal gland were significantly decreased(P<0.05),the Epo mRNA expression was significantly increased and mRNA expressions of Csf1 and Csf2 were significantly decreased in the kidney(P<0.05),the mRNA expressions of Csf3,IL-1b,IL-5 and IL-7 were significantly decreased and IL-4 mRNA expression was significantly increased in the bone marrow(P<0.05).  Conclusion:The related model of Chinese medical deficiency syndrome can be replicated by dietary restriction,administration of acetylphenylhydrazine,thyroxine and hydrocortisone. And there are corresponding changes on the material function in viscera state of kidney such as adrenal gland,kidney and bone marrow.